Abstract
BACKGROUND: The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens. RESULTS: In this study, seven endophytic bacteria were isolated from sugarcane leaves and screened for its antifungal activity against 10 fungal isolates belonging to the genera Alternaria, Cochliobolus, Curvularia, Fusarium, Neodeightonia, Phomopsis and Saccharicola isolated from diseased leaves of sugarcane. Among the seven bacterial isolates, SCB-1 showed potent antagonistic activity against the tested fungi. Based on the phenotypic data, Fatty Acid Methyl Esters (FAME) and 16S rRNA gene sequence analysis, the isolate SCB-1 was identified as Bacillus subtilis. The bacterial isolate was screened negative for chitinase production; however, chloroform and methanol extracts of the bacterial culture caused significant inhibition in the growth of the fungal isolates on semisolid media. Volatile component assay showed highest inhibitory activity against Saccharicola bicolor (SC1.4). A PCR based study detected the presence of the genes involved in biosynthesis of surfactin, bacillaene, difficidin, macrolactins and fengycin. Mass spectrometric analysis of the bacterial extract detected the presence of antifungal lipopeptide surfactin, but other metabolites were not detected. The biocontrol activity of the bacterial isolate was established when bacterial pretreated mung bean seeds were able to resist Fusarium infection, however, the untreated seeds failed to germinate. CONCLUSION: The antifungal potential of isolate Bacillus subtilis SCB-1 was established against taxonomically diverse fungal pathogens including the genera Saccharicola, Cochliobolus, Alternaria and Fusarium. The potent antifungal compound surfactin as well as volatiles produced by the bacterial isolate could be responsible for its bio-control activity against fungal infections.
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Claims 10
Claim · model-written summary
Isolate SCB-1 showed potent antagonistic activity against the tested fungi.
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e2“Among the seven bacterial isolates, SCB-1 showed potent antagonistic activity against the tested fungi”
Claim · model-written summary
Chloroform and methanol extracts significantly inhibited growth of the fungal isolates on semisolid media.
- Taxon
- sugarcanee1
- Treatment
- chloroform and methanol extractse3
- Interactor
- fungal isolatese3pathogen
- Outcome
- growth of the fungal isolatese3
- Direction
- inhibitione3
Not reportedComparator
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e3“chloroform and methanol extracts of the bacterial culture caused significant inhibition in the growth of the fungal isolates on semisolid media”
Claim · model-written summary
Volatile components of the isolate showed the highest inhibitory activity against Saccharicola bicolor.
- Taxon
- sugarcanee1
- Treatment
- Volatile componente4
- Interactor
- Saccharicola bicolorStagonospora bicolore4pathogen
- Outcome
- highest inhibitory activitye4
- Direction
- higheste4
Not reportedComparator
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e4“Volatile component assay showed highest inhibitory activity against Saccharicola bicolor (SC1.4)”
Claim · model-written summary
Mass spectrometry detected the antifungal lipopeptide surfactin in the bacterial extract.
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e5“Mass spectrometric analysis of the bacterial extract detected the presence of antifungal lipopeptide surfactin, but other metabolites were not detected”
Claim · model-written summary
Other metabolites were not detected in the bacterial extract by mass spectrometry.
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e5“Mass spectrometric analysis of the bacterial extract detected the presence of antifungal lipopeptide surfactin, but other metabolites were not detected”
Claim · model-written summary
PCR detected the presence of genes for biosynthesis of surfactin, bacillaene, difficidin, macrolactins and fengycin.
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e6“A PCR based study detected the presence of the genes involved in biosynthesis of surfactin, bacillaene, difficidin, macrolactins and fengycin”
Claim · model-written summary
The isolate was screened negative for chitinase production.
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e7“The bacterial isolate was screened negative for chitinase production”
Claim · model-written summary
Bacterial pretreatment let mung bean seeds resist Fusarium infection, whereas untreated seeds failed.
- Taxon
- mung bean
.jpg?width=60)
Vigna radiatae0 - Treatment
- bacterial pretreatede0
- Second interactor
- bacteriale0biocontrol_agent
- Outcome
- resist Fusarium infectione0
- Comparator
- untreated seedse0
FusariumNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The biocontrol activity of the bacterial isolate was established when bacterial pretreated mung bean seeds were able to resist Fusarium infection, however, the untreated seeds failed to germinate”
Claim · model-written summary
Untreated mung bean seeds failed to germinate.
- Taxon
- mung beanVigna radiatae0
- Outcome
- failed to germinatee0
- Qualifier
- untreated seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The biocontrol activity of the bacterial isolate was established when bacterial pretreated mung bean seeds were able to resist Fusarium infection, however, the untreated seeds failed to germinate”
Claim · model-written summary
Surfactin and volatiles may be responsible for the biocontrol activity of the isolate.
Verbatim
e1“seven endophytic bacteria were isolated from sugarcane leaves”
e0“The potent antifungal compound surfactin as well as volatiles produced by the bacterial isolate could be responsible for its bio-control activity against fungal infections”
The same questions in other papers
botanical · growth · Saccharum officinarum (sugarcane)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.