Abstract
WEE1 regulates the cell cycle by inactivating cyclin dependent protein kinases (CDKs) via phosphorylation. In yeast and animal cells, CDC25 phosphatase dephosphorylates the CDK releasing cells into mitosis, but in plants, its role is less clear. Expression of fission yeast CDC25 (Spcdc25) in tobacco results in small cell size, premature flowering and increased shoot morphogenetic capacity in culture. When Arath;WEE1 is over-expressed in Arabidopsis, root apical meristem cell size increases, and morphogenetic capacity of cultured hypocotyls is reduced. However expression of Arath;WEE1 in tobacco plants resulted in precocious flowering and increased shoot morphogenesis of stem explants, and in BY2 cultures cell size was reduced. This phenotype is similar to expression of Spcdc25 and is consistent with a dominant negative effect on WEE1 action. Consistent with this putative mechanism, WEE1 protein levels fell and CDKB levels rose prematurely, coinciding with early mitosis. The phenotype is not due to sense-mediated silencing of WEE1, as overall levels of WEE1 transcript were not reduced in BY2 lines expressing Arath;WEE1. However the pattern of native WEE1 transcript accumulation through the cell cycle was altered by Arath;WEE1 expression, suggesting feedback inhibition of native WEE1 transcription.
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Claims 8
Claim · model-written summary
Arath;WEE1 expression in tobacco caused precocious flowering.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- expression of Arath;WEE1e0
- Gene
- Arath;WEE1e0
- Outcome
- floweringe0
- Direction
- precociouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of Arath;WEE1 in tobacco plants resulted in precocious flowering and increased shoot morphogenesis of stem explants, and in BY2 cultures cell size was reduced.”
Claim · model-written summary
Arath;WEE1 expression in tobacco caused increased shoot morphogenesis.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- expression of Arath;WEE1e0
- Gene
- Arath;WEE1e0
- Outcome
- shoot morphogenesise0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of Arath;WEE1 in tobacco plants resulted in precocious flowering and increased shoot morphogenesis of stem explants, and in BY2 cultures cell size was reduced.”
Claim · model-written summary
Arath;WEE1 expression in tobacco caused reduced cell size.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- expression of Arath;WEE1e0
- Gene
- Arath;WEE1e0
- Outcome
- cell sizee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of Arath;WEE1 in tobacco plants resulted in precocious flowering and increased shoot morphogenesis of stem explants, and in BY2 cultures cell size was reduced.”
Claim · model-written summary
WEE1 protein levels fell prematurely.
Not reportedTaxon
Verbatim
e0“WEE1 protein levels fell and CDKB levels rose prematurely, coinciding with early mitosis.”
Claim · model-written summary
CDKB levels rose prematurely.
Not reportedTaxon
Verbatim
e0“WEE1 protein levels fell and CDKB levels rose prematurely, coinciding with early mitosis.”
Claim · model-written summary
WEE1 transcript levels were not reduced in BY2 lines expressing Arath;WEE1.
- Material
- BY2 linese0
- Treatment
- expressing Arath;WEE1e0
- Gene
- WEE1e0
- Outcome
- levels of WEE1 transcripte0
- Direction
- not reducede0
Not reportedTaxonComparator
Verbatim
e0“overall levels of WEE1 transcript were not reduced in BY2 lines expressing Arath;WEE1.”
Claim · model-written summary
Arath;WEE1 expression altered native WEE1 transcript accumulation through the cell cycle.
- Treatment
- Arath;WEE1 expressione0
- Gene
- native WEE1e0
- Outcome
- pattern of native WEE1 transcript accumulatione0
- Direction
- alterede0
Not reportedTaxonComparator
Verbatim
e0“the pattern of native WEE1 transcript accumulation through the cell cycle was altered by Arath;WEE1 expression, suggesting feedback inhibition of native WEE1 transcription.”
Claim · model-written summary
The authors infer feedback inhibition of native WEE1 transcription.
- Treatment
- Arath;WEE1 expressione0
- Gene
- native WEE1e0
- Outcome
- feedback inhibition of native WEE1 transcriptione0
Not reportedTaxon
Verbatim
e0“the pattern of native WEE1 transcript accumulation through the cell cycle was altered by Arath;WEE1 expression, suggesting feedback inhibition of native WEE1 transcription.”