Abstract
The antagonistic bacterial strain Lysobacter capsici AZ78 was recently selected for its relevant efficacy in controlling Plasmopara viticola under greenhouse conditions. Since little is known about how Lysobacter spp. may respond to desiccation, rain wash-off, and UV light, the aim of this work was to design formulations, which can effectively protect L. capsici AZ78 cells by these environmental stressors when applied in the vineyards. Firstly, fermentation conditions were optimized to achieve a harvest of 1010 L. capsici AZ78 cells/ml after 24 h and the shelf-life at 4°C indicated only a decrease of an order of magnitude after one year. Polyethyleneglycol, corn steep liquor and lignosulfonate were subsequently selected for the protection of L. capsici cells against desiccation, wash-off and UV light and were combined to implement a liquid formulation. The efficacy of L. capsici AZ78 formulated cells against P. viticola on grapevine under field conditions was higher than the unformulated ones. Finally, the fate in the environment of L. capsici AZ78 cells was assessed through a strain specific qPCR protocol. Results showed that the negative effect played by solar radiation, rainfall, unfavourable temperature and relative humidity on the unformulated AZ78 cells was mitigated by the formulation. The procedure followed in this work allowed to produce a first prototype formulation of L. capsici AZ78, that made this biocontrol agent more efficient in persisting on grapevine leaves and, consequently, controlling P. viticola under field conditions.
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Claims 5
Claim · model-written summary
Optimized fermentation gave a harvest of 1010 L. capsici AZ78 cells/ml after 24 h
Verbatim
e3“fermentation conditions were optimized to achieve a harvest of 1010 L. capsici AZ78 cells/ml after 24 h”
Claim · model-written summary
Shelf-life of L. capsici AZ78 at 4°C decreased by only an order of magnitude after one year
Verbatim
e4“the shelf-life at 4°C indicated only a decrease of an order of magnitude after one year”
Claim · model-written summary
Formulated L. capsici AZ78 cells were more efficient than unformulated cells against P. viticola on grapevine under field conditions
- Treatment
- L. capsici AZ78 formulated cellse1
- Interactor
- P. viticola
Plasmopara viticolae1pathogen - Second interactor
- L. capsici AZ78Lysobacter capsicie1biocontrol_agent
- Outcome
- efficacye1
- Direction
- highere1
- Comparator
- unformulated onese1
- Setting
- field conditionse1
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The efficacy of L. capsici AZ78 formulated cells against P. viticola on grapevine under field conditions was higher than the unformulated ones”
Claim · model-written summary
Formulation mitigated the negative effect of weather on L. capsici AZ78 cells on grapevine
- Treatment
- the formulatione2
- Outcome
- the negative effect played by solar radiation, rainfall, unfavourable temperature and relative humiditye2
- Direction
- mitigatede2
- Comparator
- unformulated AZ78 cellse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Results showed that the negative effect played by solar radiation, rainfall, unfavourable temperature and relative humidity on the unformulated AZ78 cells was mitigated by the formulation”
e5“more efficient in persisting on grapevine leaves and, consequently, controlling P. viticola under field conditions”
Claim · model-written summary
Formulation made L. capsici AZ78 more efficient in persisting on grapevine leaves
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Results showed that the negative effect played by solar radiation, rainfall, unfavourable temperature and relative humidity on the unformulated AZ78 cells was mitigated by the formulation”
e5“more efficient in persisting on grapevine leaves and, consequently, controlling P. viticola under field conditions”
The same questions in other papers
Lysobacter capsici · Plasmopara viticola · VitisResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.