Abstract
The enzyme ∆1-pyrroline-5-carboxylate synthetase (P5CS), encoded by the p5cs gene, is the rate-limiting step for proline biosynthesis, which acts as an osmoprotectant. The launch of transgenic commercial varieties depends on physiological and phenotypic characterization of events. The objective of this study was to evaluate five p5cs gene events (591, 14, 326, 164, 312), through physiological analyses and production rates during droughts. Plants of events and irrigated (IC) and stressed controls (SC) in flowering stage were submitted to 16 days of complete water restriction. The following elements were evaluated: relative water content (RWC); malondialdehyde concentration (MDA%); membrane stability index (MSI%); proline content; and production rates. As a result, the effect of water stress was only perceived after 12 days of water restriction with a decrease in the RWC values. Transgenic plants showed higher proline levels on the 16th day, compared to the SC (33 to 62%). The levels of MDA were lower on the 12th and 16th days for all the plants evaluated, but the MSI were similar to the IC for the events 591, 326, and 164, demonstrating the osmoprotectant effect. However, higher levels of proline did not result in higher production rates in the transgenic plants.
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Claims 5
Claim · model-written summary
Water stress decreased relative water content only after 12 days of water restriction.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- water stresse1
- Outcome
- RWC valuese1
- Direction
- decreasee1
- Qualifier
- only perceived after 12 days of water restrictione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat events transformed with the p5cs gene”
e1“As a result, the effect of water stress was only perceived after 12 days of water restriction with a decrease in the RWC values”
Claim · model-written summary
Transgenic p5cs wheat plants had higher proline levels than stressed controls on day 16 of water restriction, by 33 to 62%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Transgenic plantse3
- Treatment
- complete water restrictione2
- Outcome
- proline levelse3
- Direction
- highere3
- Value
- 33 to 62%e3
- Comparator
- SCe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat events transformed with the p5cs gene”
e2“Plants of events and irrigated (IC) and stressed controls (SC) in flowering stage were submitted to 16 days of complete water restriction”
e3“Transgenic plants showed higher proline levels on the 16th day, compared to the SC (33 to 62%)”
Claim · model-written summary
Malondialdehyde levels were lower on days 12 and 16 of water restriction in all wheat plants evaluated.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- complete water restrictione2
- Outcome
- levels of MDAe4
- Direction
- lowere4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat events transformed with the p5cs gene”
e2“Plants of events and irrigated (IC) and stressed controls (SC) in flowering stage were submitted to 16 days of complete water restriction”
e4“The levels of MDA were lower on the 12th and 16th days for all the plants evaluated, but the MSI were similar to the IC for the events 591, 326, and 164”
Claim · model-written summary
Membrane stability index of wheat events 591, 326 and 164 was similar to irrigated controls under water restriction.
- Taxon
- wheatTriticum aestivumt0
- Material
- events 591, 326, and 164e4
- Outcome
- MSIe4
- Direction
- no_changeinferrede4
- Comparator
- ICe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat events transformed with the p5cs gene”
e4“The levels of MDA were lower on the 12th and 16th days for all the plants evaluated, but the MSI were similar to the IC for the events 591, 326, and 164”
Claim · model-written summary
Higher proline levels did not result in higher production rates in transgenic wheat plants.
- Taxon
- wheatTriticum aestivumt0
- Material
- transgenic plantse5
- Compound
- prolinee5
- Outcome
- production ratese5
- Direction
- no_changeinferrede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat events transformed with the p5cs gene”
e5“higher levels of proline did not result in higher production rates in the transgenic plants”