Abstract
The aim of this study was to set a protocol for the in vitro culture of Curcuma longa L. Sprouted budexplants of C. longa L. were explanted on Murashige and Skoog (MS) medium supplemented with differentconcentrations of 6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1 during six subcultures. The maximum number ofmultiplied shoots (8.60 and 8.70 shoots explant-1), was obtained in the medium containing 7 mg l−1 BA at 4th and5thsubculture with (90 and 100% multiplication frequency) respectively. The optimum BA concentrations for maximumnumber of multiplication varied across subcultures. The 7 mg l-1 BA concentration at the late subculture 4th, 5th and 6thproduce highest values of (number of shoots, longest shoot, number of leaves and fresh weight).The maximum numberof roots (6.38 roots explant-1) was induced from 3 mg l−1IBA. The tallest roots (40.6 and 36.5 cm) were obtained on MSmedium supplemented with 1 mg l−1 NAA and 3 mg l−1 IBA. Also, the heaviest fresh weight was obtained frommedium containing 2 and 3 mg l−1 IBA and all concentrations of NAA. In vitro plantlets immediately acclimatized togreenhouse conditions, showing 100% survival rates in a peat moss and vermiculite (1:3) medium.
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Claims 13
Claim · model-written summary
At the fourth subculture, 7 mg l−1 BA gave 8.60 shoots per explant.
- Taxon
- Curcuma longa L.
_W_IMG_2440.jpg?width=60)
Curcuma longae2 - Treatment
- 7 mg l−1 BAe0
- Outcome
- number ofmultiplied shootse0
- Direction
- maximume0
- Value
- 8.60e0
- Qualifier
- 4the0
- Comparator
- 0, 1, 3, 5, and 7 mg l-1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_W_IMG_2440.jpg?width=500)
Verbatim
e0“The maximum number ofmultiplied shoots (8.60 and 8.70 shoots explant-1), was obtained in the medium containing 7 mg l−1 BA at 4th and5thsubculture”
e1“6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1”
e2“Curcuma longa L.”
Claim · model-written summary
At the fifth subculture, 7 mg l−1 BA gave 8.70 shoots per explant.
- Taxon
- Curcuma longa L.Curcuma longae2
- Treatment
- 7 mg l−1 BAe0
- Outcome
- number ofmultiplied shootse0
- Direction
- maximume0
- Value
- 8.70 shoots explant-1e0
- Qualifier
- 5thsubculturee0
- Comparator
- 0, 1, 3, 5, and 7 mg l-1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum number ofmultiplied shoots (8.60 and 8.70 shoots explant-1), was obtained in the medium containing 7 mg l−1 BA at 4th and5thsubculture”
e1“6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1”
e2“Curcuma longa L.”
Claim · model-written summary
At fourth subculture, 7 mg l−1 BA yielded 90% multiplication frequency.
- Taxon
- Curcuma longa L.Curcuma longae2
- Treatment
- 7 mg l−1 BAe0
- Outcome
- multiplication frequencye0
- Value
- 90e0
- Qualifier
- 4the0
- Comparator
- 0, 1, 3, 5, and 7 mg l-1e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7 mg l−1 BA at 4th and5thsubculture with (90 and 100% multiplication frequency) respectively”
e1“6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1”
e2“Curcuma longa L.”
Claim · model-written summary
At fifth subculture, 7 mg l−1 BA yielded 100% multiplication frequency.
- Taxon
- Curcuma longa L.Curcuma longae2
- Treatment
- 7 mg l−1 BAe0
- Outcome
- multiplication frequencye0
- Value
- 100%e0
- Qualifier
- 5thsubculturee0
- Comparator
- 0, 1, 3, 5, and 7 mg l-1e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7 mg l−1 BA at 4th and5thsubculture with (90 and 100% multiplication frequency) respectively”
e1“6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1”
e2“Curcuma longa L.”
Claim · model-written summary
The optimum BA concentration for multiplication number varied among subcultures.
- Taxon
- Curcuma longa L.Curcuma longae1
- Outcome
- optimum BA concentrations for maximumnumber of multiplicatione0
- Direction
- variede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum BA concentrations for maximumnumber of multiplication varied across subcultures”
e1“Curcuma longa L.”
Claim · model-written summary
Late subcultures with 7 mg l-1 BA produced the highest shoot number, longest shoots, leaf number and fresh weight.
- Taxon
- Curcuma longa L.Curcuma longae2
- Treatment
- 7 mg l-1 BAe0
- Outcome
- number of shoots, longest shoot, number of leaves and fresh weighte0
- Direction
- higheste0
- Qualifier
- late subculture 4th, 5th and 6the0
- Comparator
- 0, 1, 3, 5, and 7 mg l-1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 7 mg l-1 BA concentration at the late subculture 4th, 5th and 6thproduce highest values of (number of shoots, longest shoot, number of leaves and fresh weight)”
e1“6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1”
e2“Curcuma longa L.”
Claim · model-written summary
3 mg l−1 IBA gave the maximum root number of 6.38 per explant.
- Taxon
- Curcuma longa L.Curcuma longae1
- Treatment
- 3 mg l−1IBAe0
- Outcome
- numberof rootse0
- Direction
- maximume0
- Value
- 6.38 roots explant-1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum numberof roots (6.38 roots explant-1) was induced from 3 mg l−1IBA”
e1“Curcuma longa L.”
Claim · model-written summary
1 mg l−1 NAA produced roots 40.6 cm tall.
- Taxon
- Curcuma longa L.Curcuma longae1
- Treatment
- 1 mg l−1 NAAe0
- Outcome
- tallest rootse0
- Direction
- talleste0
- Value
- 40.6e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tallest roots (40.6 and 36.5 cm) were obtained on MSmedium supplemented with 1 mg l−1 NAA and 3 mg l−1 IBA”
e1“Curcuma longa L.”
Claim · model-written summary
3 mg l−1 IBA produced roots 36.5 cm tall.
- Taxon
- Curcuma longa L.Curcuma longae1
- Treatment
- 3 mg l−1 IBAe0
- Outcome
- tallest rootse0
- Direction
- talleste0
- Value
- 36.5 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tallest roots (40.6 and 36.5 cm) were obtained on MSmedium supplemented with 1 mg l−1 NAA and 3 mg l−1 IBA”
e1“Curcuma longa L.”
Claim · model-written summary
2 mg l−1 IBA produced the heaviest fresh weight.
- Taxon
- Curcuma longa L.Curcuma longae1
- Treatment
- 2 and 3 mg l−1 IBAe0
- Outcome
- fresh weighte0
- Direction
- heavieste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heaviest fresh weight was obtained frommedium containing 2 and 3 mg l−1 IBA and all concentrations of NAA”
e1“Curcuma longa L.”
Claim · model-written summary
3 mg l−1 IBA produced the heaviest fresh weight.
- Taxon
- Curcuma longa L.Curcuma longae1
- Treatment
- 3 mg l−1 IBAe0
- Outcome
- fresh weighte0
- Direction
- heavieste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heaviest fresh weight was obtained frommedium containing 2 and 3 mg l−1 IBA and all concentrations of NAA”
e1“Curcuma longa L.”
Claim · model-written summary
All tested NAA concentrations produced the heaviest fresh weight.
- Taxon
- Curcuma longa L.Curcuma longae1
- Treatment
- all concentrations of NAAe0
- Outcome
- fresh weighte0
- Direction
- heavieste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heaviest fresh weight was obtained frommedium containing 2 and 3 mg l−1 IBA and all concentrations of NAA”
e1“Curcuma longa L.”
Claim · model-written summary
In vitro plantlets showed 100% survival after immediate greenhouse acclimatization in peat moss and vermiculite medium.
- Taxon
- Curcuma longa L.Curcuma longae1
- Organ / stage
- In vitro plantletse0
- Treatment
- peat moss and vermiculite (1:3) mediume0
- Outcome
- survival ratese0
- Value
- 100%e0
- Setting
- greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro plantlets immediately acclimatized togreenhouse conditions, showing 100% survival rates in a peat moss and vermiculite (1:3) medium”
e1“Curcuma longa L.”
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