Abstract
The experiment aimed to induction of insensitive peanut somatic embryo (SE) to culture filtrate medium from various races of S. rolfsii and its plantlet regeneration. To achieve these objective, the experiments have been done in several steps: somatic embryo development, preparation of culture filtrate (CF) medium from various races of S. rolfsii, induction of insensitive peanut somatic embryo and peanut plantlet regeneration of insensitive somatic embryo. Result of experiment showed that selected SEs on selective medium containing culture filtrate of S. rolfsii one race or two races or three races or four races were specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsii. All SEs selected in culture filtrate medium of four races S. rolfsii (race 1, 6, 8, and 9) were more insensitive on culture filtrate medium of S. rolfsii races compared with SEs that only selected in culture filtrate medium of one race. Selected insensitive SEs had ability to form normal seedling (50.5-65.5%) and abnormal germinated SEs (23.5 - 30.5%). Normal germinated SEs developed to form plantlets. These plantlets were developed to produced peanut plant R1 generation and peanut seeds. Further evaluation using subsequent generation of peanut (R1 and R2) plants need to be conducted to testing peanut line resistance to stem rot disease (S. rolfsii) in the field.
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Claims 7
Claim · model-written summary
Embryos selected with one to four S. rolfsii races were insensitive specifically to the race combinations used in selection.
- Taxon
- peanut
Arachis hypogaeat0 - Interactor
- S. rolfsii
Agroathelia rolfsiie0pathogen - Outcome
- specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsiie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“selected SEs on selective medium containing culture filtrate of S. rolfsii one race or two races or three races or four races were specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsii”
t0“peanut”
Claim · model-written summary
Embryos selected with four S. rolfsii races were more insensitive than those selected with one race.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- culture filtrate medium of four races S. rolfsiie0
- Interactor
- S. rolfsiiAgroathelia rolfsiie0pathogen
- Outcome
- insensitive on culture filtrate medium of S. rolfsii racese0
- Direction
- moree0
- Comparator
- SEs that only selected in culture filtrate medium of one racee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All SEs selected in culture filtrate medium of four races S. rolfsii (race 1, 6, 8, and 9) were more insensitive on culture filtrate medium of S. rolfsii races compared with SEs that only selected in culture filtrate medium of one race”
t0“peanut”
Claim · model-written summary
Selected insensitive embryos formed normal seedlings at 50.5-65.5%.
- Taxon
- peanutArachis hypogaeat0
- Outcome
- form normal seedlinge0
- Value
- 50.5-65.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selected insensitive SEs had ability to form normal seedling (50.5-65.5%)”
t0“peanut”
Claim · model-written summary
Selected insensitive embryos also germinated abnormally at 23.5 - 30.5%.
- Taxon
- peanutArachis hypogaeat0
- Outcome
- abnormal germinated SEse0
- Value
- 23.5 - 30.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“abnormal germinated SEs (23.5 - 30.5%)”
t0“peanut”
Claim · model-written summary
Normal germinated embryos developed into plantlets.
- Taxon
- peanutArachis hypogaeat0
- Outcome
- developed to form plantletse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Normal germinated SEs developed to form plantlets”
t0“peanut”
Claim · model-written summary
Regenerated plantlets produced R1 peanut plants and seeds.
- Taxon
- peanutArachis hypogaeae0
- Outcome
- peanut plant R1 generation and peanut seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plantlets were developed to produced peanut plant R1 generation and peanut seeds”
Claim · model-written summary
The authors recommend evaluating R1 and R2 plants for field stem rot resistance.
- Taxon
- peanutArachis hypogaeae0
- Interactor
- S. rolfsiiAgroathelia rolfsiie0pathogen
- Outcome
- testing peanut line resistance to stem rot diseasee0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further evaluation using subsequent generation of peanut (R1 and R2) plants need to be conducted to testing peanut line resistance to stem rot disease (S. rolfsii) in the field”
The same questions in other papers
pathogen product · organism · Arachis hypogaea (peanut)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.