Abstract
It has been reported that squash leaf curl China virus (SLCCNV) infects some Cucurbitaceae crops except for melon (Cucumis melo L.). A new disease of melon exhibiting severe leaf curl and dwarfing was observed in Hainan Province of China. In this study, the pathogen was identified as SLCCNV through biological and molecular characterization. The isolate (SLCCNV-HN) possess a bipartite genome, DNA-A (HM566112.1) with the highest nucleotide identity (99%) to SLCCNV-Hn (MF062251.1) pumpkin and SLCCNV-Hn61 (AM260205.1) squash isolates from China, whereas DNA-B (HM566113.1) with the highest nucleotide identity (99%) to SLCCNV-Hn (MF062252.1). Phylogenetic analyses based on the full-length SLCCNV-HN DNA-A and -B sequences indicated that SLCCNV-HN melon isolate is clustered with SLCCNV-Hn pumpkin, SLCCNV-Hn61 and SLCCNV-SY squash isolates from southern China, forming an independent cluster. Infectious clone of SLCCNV-HN was constructed and the melon plants were inoculated and the infection rate is 100%, the systemic symptoms in melon showed identical to those of melon plants infected in fields. Additionally, melon plants transmission of this virus by Bemisia tabaci with a transmission rate of 95% (19/20) showed leaf curl and dwarf symptoms 15 days post transmission, thereby fulfilling Koch's postulates. Analysis of genomic organization and phylogenetic trees indicated that SLCCNV-HN melon isolate belongs to the Begomovirus genus. To the best of our knowledge, this is the first characterization of melon-infecting SLCCNV through its genome, infectious clone and transmission.
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Claims 11
Claim · model-written summary
A new melon disease with leaf curl and dwarfing occurred in Hainan.
- Taxon
- melon
.jpg?width=60)
Cucumis meloe0 - Outcome
- severe leaf curl and dwarfinge0
- Setting
- Hainan Province of Chinae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A new disease of melon exhibiting severe leaf curl and dwarfing was observed in Hainan Province of China”
Claim · model-written summary
The melon pathogen was identified as SLCCNV.
- Taxon
- melonCucumis melot0
- Interactor
- SLCCNVe0pathogen
- Outcome
- pathogen was identified as SLCCNVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pathogen was identified as SLCCNV through biological and molecular characterization”
t0“melon”
Claim · model-written summary
SLCCNV-HN has a bipartite genome.
- Taxon
- melonCucumis melot0
- Interactor
- SLCCNV-HNe0pathogen
- Outcome
- bipartite genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The isolate (SLCCNV-HN) possess a bipartite genome”
t0“melon”
Claim · model-written summary
SLCCNV-HN DNA-A had 99% highest identity to pumpkin and squash isolates.
- Taxon
- melonCucumis melot0
- Interactor
- SLCCNV-Hne0pathogen
- Gene
- DNA-Ae0
- Outcome
- nucleotide identitye0
- Direction
- higheste0
- Value
- 99%e0
- Comparator
- SLCCNV-Hn (MF062251.1) pumpkin and SLCCNV-Hn61 (AM260205.1) squash isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA-A (HM566112.1) with the highest nucleotide identity (99%) to SLCCNV-Hn (MF062251.1) pumpkin and SLCCNV-Hn61 (AM260205.1) squash isolates from China”
t0“melon”
Claim · model-written summary
SLCCNV-HN DNA-B had 99% highest identity to SLCCNV-Hn.
- Taxon
- melonCucumis melot0
- Interactor
- SLCCNV-Hne0pathogen
- Gene
- DNA-Be0
- Outcome
- nucleotide identitye0
- Direction
- higheste0
- Value
- 99%e0
- Comparator
- SLCCNV-Hn (MF062252.1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA-B (HM566113.1) with the highest nucleotide identity (99%) to SLCCNV-Hn (MF062252.1)”
t0“melon”
Claim · model-written summary
SLCCNV-HN grouped with southern China pumpkin and squash isolates in an independent cluster.
- Taxon
- melonCucumis meloe0
- Interactor
- SLCCNV-HNe0pathogen
- Outcome
- independent clustere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SLCCNV-HN melon isolate is clustered with SLCCNV-Hn pumpkin, SLCCNV-Hn61 and SLCCNV-SY squash isolates from southern China, forming an independent cluster”
Claim · model-written summary
Infectious clone inoculation infected all melon plants.
- Taxon
- melonCucumis meloe0
- Treatment
- Infectious clone of SLCCNV-HNe0
- Interactor
- SLCCNV-HNe0pathogen
- Outcome
- infection ratee0
- Value
- 100%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infectious clone of SLCCNV-HN was constructed and the melon plants were inoculated and the infection rate is 100%”
Claim · model-written summary
Clone inoculated melon symptoms matched field symptoms.
- Taxon
- melonCucumis meloe0
- Outcome
- systemic symptomse0
- Direction
- identicale0
- Comparator
- melon plants infected in fieldse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the systemic symptoms in melon showed identical to those of melon plants infected in fields”
Claim · model-written summary
Bemisia tabaci transmitted the virus to melon at 95%.
- Taxon
- melonCucumis meloe0
- Interactor
- Bemisia tabaci
Bemisia tabacie0pest - Outcome
- transmission ratee0
- Value
- 95% (19/20)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melon plants transmission of this virus by Bemisia tabaci with a transmission rate of 95% (19/20)”
Claim · model-written summary
Transmitted virus produced leaf curl and dwarf symptoms after 15 days.
- Taxon
- melonCucumis melot0
- Outcome
- leaf curl and dwarf symptomse0
- Value
- 15 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“showed leaf curl and dwarf symptoms 15 days post transmission”
t0“melon”
Claim · model-written summary
Genomic and phylogenetic analysis placed SLCCNV-HN in Begomovirus.
- Taxon
- melonCucumis meloe0
- Interactor
- SLCCNV-HNe0pathogen
- Outcome
- belongs to the Begomovirus genuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SLCCNV-HN melon isolate belongs to the Begomovirus genus”
The same questions in other papers
infection · disease · Cucumis melo (muskmelon)Results agree
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.