Abstract
Watermelon mosaic virus (WMV), a member of the genus Potyvirus, causes serious economic losses in cucurbit crops. For molecular biological studies of viruses, it is necessary to construct an infectious clone that can facilitate gene functional analysis. In this study, we constructed an infectious cDNA clone of WMV genomic RNA by Gibson assembly and evaluated its virulence and symptoms on a variety of host plants. A WMV isolate (WMV-IS-me2) collected from melon in Iksan, Jeonbuk Province, Korea in 2015 caused mosaic symptoms on leaves. Four overlapping PCR fragments of the full-length genome of this isolate and the pJL89 binary vector with overhanging ends of 40 bp were amplified by reverse transcription PCR and joined in a single isothermal reaction. The complete nucleotide sequence of the infectious cDNA clone of WMV was determined and the recombinant vector was transformed into Agrobacterium tumefaciens GV3101. Following agro-infiltration, an infectious clone (pWMV-M, KACC95145P) systemically induced mosaic symptoms on watermelon (Citrullus lanatus), zucchini (Cucurbita pepo), melon (Cucumis melo), and Nicotiana benthamiana plants. This infectious clone may be useful for screening cucurbit varieties resistant to WMV, as well as for studying viral gene function.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
The WMV isolate from melon caused mosaic symptoms on leaves.
- Taxon
- melon
.jpg?width=60)
Cucumis meloe0 - Interactor
- WMVe0pathogen
- Outcome
- mosaic symptoms on leavese0
- Setting
- Iksan, Jeonbuk Province, Koreae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A WMV isolate (WMV-IS-me2) collected from melon in Iksan, Jeonbuk Province, Korea in 2015 caused mosaic symptoms on leaves”
Claim · model-written summary
The infectious clone systemically induced mosaic symptoms on watermelon.
- Taxon
- watermelon
Citrullus lanatuse0 - Treatment
- infectious clone (pWMV-M, KACC95145P)e0
- Interactor
- infectious clonee0pathogen
- Outcome
- mosaic symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an infectious clone (pWMV-M, KACC95145P) systemically induced mosaic symptoms on watermelon (Citrullus lanatus), zucchini (Cucurbita pepo), melon (Cucumis melo), and Nicotiana benthamiana plants”
Claim · model-written summary
The infectious clone systemically induced mosaic symptoms on zucchini.
- Taxon
- zucchini
Cucurbita pepo subsp. pepoe0 - Treatment
- infectious clone (pWMV-M, KACC95145P)e0
- Interactor
- infectious clonee0pathogen
- Outcome
- mosaic symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an infectious clone (pWMV-M, KACC95145P) systemically induced mosaic symptoms on watermelon (Citrullus lanatus), zucchini (Cucurbita pepo), melon (Cucumis melo), and Nicotiana benthamiana plants”
Claim · model-written summary
The infectious clone systemically induced mosaic symptoms on melon.
- Taxon
- melonCucumis meloe0
- Treatment
- infectious clone (pWMV-M, KACC95145P)e0
- Interactor
- infectious clonee0pathogen
- Outcome
- mosaic symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an infectious clone (pWMV-M, KACC95145P) systemically induced mosaic symptoms on watermelon (Citrullus lanatus), zucchini (Cucurbita pepo), melon (Cucumis melo), and Nicotiana benthamiana plants”
Claim · model-written summary
The infectious clone systemically induced mosaic symptoms on Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- infectious clone (pWMV-M, KACC95145P)e0
- Interactor
- infectious clonee0pathogen
- Outcome
- mosaic symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an infectious clone (pWMV-M, KACC95145P) systemically induced mosaic symptoms on watermelon (Citrullus lanatus), zucchini (Cucurbita pepo), melon (Cucumis melo), and Nicotiana benthamiana plants”
Claim · model-written summary
The complete nucleotide sequence of the WMV infectious clone was determined.
- Taxon
- melonCucumis melot0
- Interactor
- WMVe0pathogen
- Outcome
- complete nucleotide sequencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The complete nucleotide sequence of the infectious cDNA clone of WMV was determined”
t0“Isolated from Melon”
Claim · model-written summary
The authors suggest using the infectious clone to screen cucurbit varieties for WMV resistance.
Verbatim
e0“This infectious clone may be useful for screening cucurbit varieties resistant to WMV”
The same questions in other papers
infection · disease · Cucurbita pepoPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
infection · disease · Citrullus lanatus (watermelon)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
5 papers more in this group
See all 9 papers →infection · disease · Nicotiana benthamianaPositive and negative results
12 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
15 papers more in this group
See all 19 papers →infection · disease · Cucumis melo (muskmelon)Results agree
5 papers in the same direction as this paper
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.