Abstract
Cucumber mosaic virus (CMV), with extremely broad host range including both monocots and dicots around the world, belongs to most important viral crop threats. Either natural or genetically constructed sources of resistance are being intensively investigated; for this purpose, exhaustive knowledge of molecular virus-host interaction during compatible and incompatible infection is required. New technologies and computer-based "omics" on various levels contribute markedly to this topic. In this work, two cucumber cultivars with different response to CMV challenge were tested, i.e., sensitive cv. Vanda and resistant cv. Heliana. The transcriptomes were prepared from both cultivars at 18 days after CMV or mock inoculation. Subsequently, four independent comparative analyses of obtained data were performed, viz. mock- and CMV-inoculated samples within each cultivar, samples from mock-inoculated cultivars to each other and samples from virus-inoculated cultivars to each other. A detailed picture of CMV-influenced genes, as well as constitutive differences in cultivar-specific gene expression was obtained. The compatible CMV infection of cv. Vanda caused downregulation of genes involved in photosynthesis, and induction of genes connected with protein production and modification, as well as components of signaling pathways. CMV challenge caused practically no change in the transcription profile of the cv. Heliana. The main differences between constitutive transcription activity of the two cultivars relied in the expression of genes responsible for methylation, phosphorylation, cell wall organization and carbohydrate metabolism (prevailing in cv. Heliana), or chromosome condensation and glucan biosynthesis (prevailing in cv. Vanda). Involvement of several genes in the resistant cucumber phenotype was predicted; this can be after biological confirmation potentially applied in breeding programs for virus-resistant crops.
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Claims 6
Claim · model-written summary
Compatible CMV infection downregulated photosynthesis genes in cucumber cv. Vanda relative to mock inoculation.
- Taxon
- cucumber
Cucumis sativuse0 - Material
- cv. Vandae3
- Treatment
- compatible CMV infectione3
- Interactor
- Cucumber mosaic virus
cucumber mosaic cucumoviruse1pathogen - Outcome
- genes involved in photosynthesise3
- Direction
- downregulatione3
- Comparator
- mock inoculatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two cucumber cultivars with different response to CMV challenge were tested”
e1“Cucumber mosaic virus (CMV), with extremely broad host range”
e2“The transcriptomes were prepared from both cultivars at 18 days after CMV or mock inoculation”
e3“The compatible CMV infection of cv. Vanda caused downregulation of genes involved in photosynthesis, and induction of genes connected with protein production and modification, as well as components of signaling pathways”
Claim · model-written summary
Compatible CMV infection induced genes for protein production and modification and components of signaling pathways in cucumber cv. Vanda.
- Taxon
- cucumberCucumis sativuse0
- Material
- cv. Vandae3
- Treatment
- compatible CMV infectione3
- Interactor
- Cucumber mosaic viruscucumber mosaic cucumoviruse1pathogen
- Outcome
- genes connected with protein production and modification, as well as components of signaling pathwayse3
- Direction
- inductione3
- Comparator
- mock inoculatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two cucumber cultivars with different response to CMV challenge were tested”
e1“Cucumber mosaic virus (CMV), with extremely broad host range”
e2“The transcriptomes were prepared from both cultivars at 18 days after CMV or mock inoculation”
e3“The compatible CMV infection of cv. Vanda caused downregulation of genes involved in photosynthesis, and induction of genes connected with protein production and modification, as well as components of signaling pathways”
Claim · model-written summary
CMV challenge caused practically no change in the transcription profile of cucumber cv. Heliana.
- Taxon
- cucumberCucumis sativuse0
- Material
- cv. Helianae4
- Treatment
- CMV challengee4
- Interactor
- Cucumber mosaic viruscucumber mosaic cucumoviruse1pathogen
- Outcome
- transcription profilee4
- Direction
- no changee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two cucumber cultivars with different response to CMV challenge were tested”
e1“Cucumber mosaic virus (CMV), with extremely broad host range”
e4“CMV challenge caused practically no change in the transcription profile of the cv. Heliana”
Claim · model-written summary
Genes for methylation, phosphorylation, cell wall organization and carbohydrate metabolism were expressed more in cv. Heliana than cv. Vanda.
- Taxon
- cucumberCucumis sativuse0
- Material
- cv. Helianae5
- Outcome
- expression of genes responsible for methylation, phosphorylation, cell wall organization and carbohydrate metabolisme5
- Direction
- prevailinge5
- Comparator
- cv. Vandae5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two cucumber cultivars with different response to CMV challenge were tested”
e5“The main differences between constitutive transcription activity of the two cultivars relied in the expression of genes responsible for methylation, phosphorylation, cell wall organization and carbohydrate metabolism (prevailing in cv. Heliana), or chromosome condensation and glucan biosynthesis (prevailing in cv. Vanda)”
Claim · model-written summary
Chromosome condensation and glucan biosynthesis genes were expressed more in cv. Vanda than cv. Heliana.
- Taxon
- cucumberCucumis sativuse0
- Material
- cv. Vandae5
- Outcome
- chromosome condensation and glucan biosynthesise5
- Direction
- prevailinge5
- Comparator
- cv. Helianae5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two cucumber cultivars with different response to CMV challenge were tested”
e5“The main differences between constitutive transcription activity of the two cultivars relied in the expression of genes responsible for methylation, phosphorylation, cell wall organization and carbohydrate metabolism (prevailing in cv. Heliana), or chromosome condensation and glucan biosynthesis (prevailing in cv. Vanda)”
Claim · model-written summary
Several genes are predicted to be involved in the resistant cucumber phenotype.
- Taxon
- cucumberCucumis sativuse0
- Outcome
- resistant cucumber phenotypee6
- Qualifier
- Involvement of several genese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two cucumber cultivars with different response to CMV challenge were tested”
e6“Involvement of several genes in the resistant cucumber phenotype was predicted”
The same questions in other papers
infection · cucumber mosaic cucumovirus · Cucumis sativus (cucumber)Results differ
5 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.