Abstract
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important and devastating diseases of wheat; therefore, it is necessary to rapidly and accurately quantify fungicide effectiveness to monitor Pst sensitivity and manage the disease. In this study, a rapid method of quantifying the fungicide effectiveness with detached leaves was developed. The results showed that 0.5% water agar containing 75 μg/ml of 6-benzylaminopurine and filter paper worked the best for maintaining wheat leaves. The disease incidences of different concentrations of spore suspension were compared. When the spore concentrations were 5 and 10 mg/ml, the disease incidences had no significant differences at 12 and 15 days after inoculation (P < 0.05). Fungicide treatment tests revealed that there were no significant differences in the efficacies of triadimefon on rust suppression between detached leaves in the culture dishes and direct spray on seedlings. We also developed a Photoshop software method that can replace the current classification method and accurately measure the proportion of sporulation area on infected leaves. The sensitivity baseline of Pst to triadimefon was estimated as 0.1453 ± 0.0081 μg/ml, and all the values of EC 50 were tested for normal distribution using the Shapiro–Wilk test (W = 0.204). The baseline can be used to test the sensitivity of different Pst isolates to triadimefon.
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Claims 5
Claim · model-written summary
A detached-leaf medium of 0.5% water agar with 75 μg/ml 6-benzylaminopurine and filter paper worked best for maintaining wheat leaves.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- 0.5% water agar containing 75 μg/ml of 6-benzylaminopurinee1
- Outcome
- maintaining wheat leavese1
- Direction
- worked the beste1
- Value
- 75 μg/mle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Inhibiting Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici)”
e1“0.5% water agar containing 75 μg/ml of 6-benzylaminopurine and filter paper worked the best for maintaining wheat leaves”
Claim · model-written summary
Spore suspension concentrations of 5 and 10 mg/ml gave no significant difference in wheat stripe rust incidence at 12 and 15 days after inoculation.
- Taxon
- wheatTriticum aestivume0
- Treatment
- spore concentrationse2
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Outcome
- disease incidencese2
- Direction
- no_changeinferrede2
- Value
- 5 and 10 mg/mle2
- Qualifier
- at 12 and 15 days after inoculatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important and devastating diseases of wheat”
e2“When the spore concentrations were 5 and 10 mg/ml, the disease incidences had no significant differences at 12 and 15 days after inoculation”
Claim · model-written summary
Fungicide efficacy of triadimefon on rust suppression did not differ between detached leaves and direct spray on seedlings.
- Taxon
- wheatTriticum aestivume0
- Treatment
- triadimefone3
- Outcome
- efficacies of triadimefon on rust suppressione3
- Direction
- no_changeinferrede3
- Comparator
- direct spray on seedlingse3
- Setting
- detached leaves in the culture dishese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important and devastating diseases of wheat”
e3“there were no significant differences in the efficacies of triadimefon on rust suppression between detached leaves in the culture dishes and direct spray on seedlings”
Claim · model-written summary
A Photoshop method can replace the current classification method and accurately measure the proportion of sporulation area on infected wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Outcome
- proportion of sporulation area on infected leavese4
- Comparator
- current classification methode4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important and devastating diseases of wheat”
e4“a Photoshop software method that can replace the current classification method and accurately measure the proportion of sporulation area on infected leaves”
Claim · model-written summary
The sensitivity baseline of Pst to triadimefon was 0.1453 ± 0.0081 μg/ml.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pste5pathogen
- Outcome
- sensitivity baseline of Pst to triadimefone5
- Value
- 0.1453 ± 0.0081 μg/mle5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important and devastating diseases of wheat”
e5“The sensitivity baseline of Pst to triadimefon was estimated as 0.1453 ± 0.0081 μg/ml”
The same questions in other papers
triadimefon · disease · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 6 reporting no difference from an alternative
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.