Abstract
Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmoA gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland. High-throughput sequencing of pmoA based on DNA and cDNA was used to analyze the methanotrophic community. Sequencing of cDNA pmoA amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter. In T. angustifolia, these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland.
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Claims 7
Claim · model-written summary
Methylomonas was the most active methanotroph group in the roots of Phragmites australis.
- Taxon
- Phragmites australis
Phragmites australise0 - Organ / stage
- root samplese0
- Interactor
- Methylomonas
Methylomonase1symbiont - Outcome
- most active groupe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland”
e1“A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter”
Claim · model-written summary
Methylocystis was the dominant methanotroph group in the roots of Schoenoplectus triqueter.
- Taxon
- Schoenoplectus triqueter
Schoenoplectus triquetere0 - Organ / stage
- root samplese0
- Interactor
- MethylocystisMethylocystise1symbiont
- Outcome
- dominant groupe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland”
e1“A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter”
Claim · model-written summary
Type I and type II methanotrophs existed in similar proportions in the roots of Typha angustifolia.
- Taxon
- Typha angustifolia
.jpg?width=60)
Typha angustifoliae0 - Interactor
- two types of methanotrophe2symbiont
- Outcome
- proportionse2
- Qualifier
- similare2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland”
e2“In T. angustifolia, these two types of methanotroph existed in similar proportions”
Claim · model-written summary
At the DNA level, Methylomonas was predominant in the roots of all three emergent macrophytes.
- Taxon
- three emergent macrophytese0
- Organ / stage
- rootse3
- Interactor
- MethylomonasMethylomonase3symbiont
- Outcome
- predominante3
- Qualifier
- at the DNA levele3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland”
e3“However, at the DNA level, Methylomonas was predominant in the roots of all three plants”
Claim · model-written summary
Vegetation type may affect the root-associated methanotrophic community at both DNA and cDNA levels.
- Taxon
- three emergent macrophytese0
- Treatment
- vegetation typee4
- Outcome
- root-associated methanotrophic communitye4
- Qualifier
- at both DNA and cDNA levelse4
Not reportedDirection
Verbatim
e0“from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland”
e4“vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels”
Claim · model-written summary
The structure of active methanotrophs in cDNA was not always consistent with the DNA-based structure.
Verbatim
e0“from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland”
e6“Sequencing of cDNA pmoA amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA”
Claim · model-written summary
Methylomonas and Methylocystis likely contribute to aerobic methane oxidation in the eutrophic wetland roots.
- Taxon
- three emergent macrophytese0
- Interactor
- MethylomonasMethylomonase5symbiont
- Second interactor
- MethylocystisMethylocystise5symbiont
- Outcome
- aerobic methane oxidatione5
- Qualifier
- in a eutrophic wetlande5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland”
e5“These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland”