Abstract
Rice is a main food crop, but sensitive to salinity. The high affinity K + transporters (HKTs) have been proven to be important to salt tolerance in plants. In the current study, the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice. Sequence analysis of OsHKT1;1 gene was conducted in the coding and promoter regions of all investigated rice cultivars. There were nine SNPs in the coding sequences and ten SNPs in the promoter sequences. Amongst nine SNPs found in the coding sequences, four were nonsynonymous (C89T, C280T, T536C, A773G) leading to four substituted amino acids P30L, L94F, F179S, and N258S. In silico analysis revealed no potential effects of the substituted amino acids to protein structure, but caused changes in post-translational modifications. In the promoter sequences, eight out of ten SNPs caused to five additions and six deletions of the cis-regulatory elements, in which some of them were shown to be involved in stress responses. OsHKT1;1 gene expression was analyzed in the roots and leaves in response to different salt concentrations. While the expression of OsHKT1;1 was induced in the leaves at the beginning of salt treatment (day 1 and day 2), it was decreased or unchanged in the roots.
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Claims 9
Claim · model-written summary
Nine SNPs were found in the OsHKT1;1 coding sequences.
- Taxon
- rice
Oryza sativae0 - Gene
- OsHKT1;1e0
- Outcome
- SNPs in the coding sequencese1
- Value
- ninee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e1“There were nine SNPs in the coding sequences and ten SNPs in the promoter sequences”
Claim · model-written summary
Ten SNPs were found in the OsHKT1;1 promoter sequences.
- Taxon
- riceOryza sativae0
- Gene
- OsHKT1;1e0
- Outcome
- SNPs in the promoter sequencese1
- Value
- tene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e1“There were nine SNPs in the coding sequences and ten SNPs in the promoter sequences”
Claim · model-written summary
Four of the nine coding-sequence SNPs were nonsynonymous.
- Taxon
- riceOryza sativae0
- Gene
- OsHKT1;1e0
- Outcome
- nonsynonymouse2
- Value
- foure2
- Qualifier
- Amongst nine SNPs found in the coding sequencese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e2“Amongst nine SNPs found in the coding sequences, four were nonsynonymous (C89T, C280T, T536C, A773G) leading to four substituted amino acids P30L, L94F, F179S, and N258S”
Claim · model-written summary
The nonsynonymous SNPs changed four amino acids: P30L, L94F, F179S and N258S.
- Taxon
- riceOryza sativae0
- Gene
- OsHKT1;1e0
- Outcome
- substituted amino acidse2
- Value
- foure2
- Qualifier
- P30L, L94F, F179S, and N258Se2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e2“Amongst nine SNPs found in the coding sequences, four were nonsynonymous (C89T, C280T, T536C, A773G) leading to four substituted amino acids P30L, L94F, F179S, and N258S”
Claim · model-written summary
In silico, the substituted amino acids had no potential effect on protein structure.
- Taxon
- riceOryza sativae0
- Gene
- OsHKT1;1e0
- Outcome
- protein structuree3
- Qualifier
- no potential effectse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e3“In silico analysis revealed no potential effects of the substituted amino acids to protein structure, but caused changes in post-translational modifications”
Claim · model-written summary
In silico, the substituted amino acids were predicted to change post-translational modifications.
- Taxon
- riceOryza sativae0
- Gene
- OsHKT1;1e0
- Outcome
- post-translational modificationse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e3“In silico analysis revealed no potential effects of the substituted amino acids to protein structure, but caused changes in post-translational modifications”
Claim · model-written summary
Eight of ten promoter SNPs caused five additions and six deletions of cis-regulatory elements.
- Taxon
- riceOryza sativae0
- Gene
- OsHKT1;1e0
- Outcome
- cis-regulatory elementse4
- Value
- five additions and six deletionse4
- Qualifier
- eight out of ten SNPse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e4“In the promoter sequences, eight out of ten SNPs caused to five additions and six deletions of the cis-regulatory elements, in which some of them were shown to be involved in stress responses”
Claim · model-written summary
OsHKT1;1 expression was induced in rice leaves at the beginning of salt treatment (day 1 and day 2).
- Taxon
- riceOryza sativae0
- Organ / stage
- leavese5
- Treatment
- salt treatmente5
- Gene
- OsHKT1;1e5
- Outcome
- expressione5
- Direction
- inducede5
- Qualifier
- (day 1 and day 2)e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e5“While the expression of OsHKT1;1 was induced in the leaves at the beginning of salt treatment (day 1 and day 2), it was decreased or unchanged in the roots”
Claim · model-written summary
OsHKT1;1 expression in rice roots was decreased or unchanged during salt treatment.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse5
- Treatment
- salt treatmente5
- Gene
- OsHKT1;1e5
- Outcome
- expressione5
- Direction
- decreased or unchangede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the natural variations in gene sequence of OsHKT1;1 were investigated for uncovering the potential allelic variants in salt tolerance in rice”
e5“While the expression of OsHKT1;1 was induced in the leaves at the beginning of salt treatment (day 1 and day 2), it was decreased or unchanged in the roots”