Abstract
An efficient protocol was developed for mass scale in vitro corm production of Gladiolus (cv. ‘White Friendship’) using liquid culture and coconut coir explored as a matrix. The culture was initiated from the basal portion of the innermost leaf of the sprout. The cut surface of responding explant was swelled in MS solid basal media supplemented with 2 mg L-1 NAA. Ten to fifteen shoot buds were observed when the responding explant was transferred in MS media with 2 mg L-1 BAP and 0.2 mg L-1 NAA. The high frequency of in vitro corms were initiated in liquid MS media supplemented with 0.5 mg L-1 NAA and 6% sucrose in coir matrix. The corms with highest mean fresh weight (4570 mg) and diameter (25 mm) developed on periodically replaced media (every three weeks) than unchanged media. Using RAPD profiling, the genetic fidelity of in vitro raised corms were tested.
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Claims 4
Claim · model-written summary
MS medium with BAP and NAA produced ten to fifteen shoot buds.
GladiolusNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ten to fifteen shoot buds were observed when the responding explant was transferred in MS media with 2 mg L-1 BAP and 0.2 mg L-1 NAA”
t0“Gladiolus”
Claim · model-written summary
Liquid MS medium with NAA and sucrose in coir matrix initiated in vitro corms at high frequency.
- Treatment
- liquid MS media supplemented with 0.5 mg L-1 NAA and 6% sucrose in coir matrixe0
- Outcome
- frequency of in vitro cormse0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high frequency of in vitro corms were initiated in liquid MS media supplemented with 0.5 mg L-1 NAA and 6% sucrose in coir matrix”
t0“Gladiolus”
Claim · model-written summary
Periodic media replacement produced corms with the highest mean fresh weight of 4570 mg compared with unchanged medium.
- Treatment
- periodically replaced media (every three weeks)e0
- Outcome
- mean fresh weighte0
- Direction
- higheste0
- Value
- 4570 mge0
- Comparator
- unchanged mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The corms with highest mean fresh weight (4570 mg) and diameter (25 mm) developed on periodically replaced media (every three weeks) than unchanged media”
t0“Gladiolus”
Claim · model-written summary
Periodic media replacement produced corms with a diameter of 25 mm compared with unchanged medium.
- Treatment
- periodically replaced media (every three weeks)e0
- Outcome
- diametere0
- Direction
- higheste0
- Value
- 25 mme0
- Comparator
- unchanged mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The corms with highest mean fresh weight (4570 mg) and diameter (25 mm) developed on periodically replaced media (every three weeks) than unchanged media”
t0“Gladiolus”
The same questions in other papers
in vitro · growth · GladiolusResults agree
13 papers in the same direction as this paper
- positive
- positive
- positive
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.