Abstract
Rhizoctonia cerealis is the causal pathogen of the devastating disease, sharp eyespot, of the important crop wheat (Triticum aestivum L.). In phytopathogenic fungi, several M36 metalloproteases have been implicated in virulence, but pathogenesis roles of M35 family metalloproteases are largely unknown. Here, we identified four M35 family metalloproteases from R. cerealis genome, designated RcMEP2–RcMEP5, measured their transcriptional profiles, and investigated RcMEP2 function. RcMEP2-RcMEP5 are predicted as secreted metalloproteases since each protein sequence contains a signal peptide and an M35 domain that includes two characteristic motifs HEXXE and GTXDXXYG. Transcription levels of RcMEP2-RcMEP5 markedly elevated during the fungus infection to wheat, among which RcMEP2 expressed with the highest level. Functional dissection indicated that RcMEP2 and its M35 domain could trigger H2O2 rapidly-excessive accumulation, induce cell death, and inhibit expression of host chitinases. This consequently enhanced the susceptibility of wheat to R. cerealis and the predicated signal peptide of RcMEP2 functions required for secretion and cell death-induction. These results demonstrate that RcMEP2 is a virulence factor and that its M35 domain and signal peptide are necessary for the virulence role of RcMEP2. This study facilitates a better understanding of the pathogenesis mechanism of metalloproteases in phytopathogens including R. cerealis.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Four M35 metalloproteases were identified in R. cerealis.
- Taxon
- Wheat
Triticum aestivumt0 - Interactor
- R. cerealis
Ceratobasidium cerealee0pathogen - Gene
- RcMEP2–RcMEP5e0
- Outcome
- M35 family metalloproteasese0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified four M35 family metalloproteases from R. cerealis genome, designated RcMEP2–RcMEP5”
t0“Wheat”
Claim · model-written summary
RcMEP2-RcMEP5 were predicted secreted metalloproteases.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- Rhizoctonia cerealisCeratobasidium cerealet0pathogene0
- Gene
- RcMEP2-RcMEP5e0
- Outcome
- secreted metalloproteasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RcMEP2-RcMEP5 are predicted as secreted metalloproteases since each protein sequence contains a signal peptide and an M35 domain that includes two characteristic motifs HEXXE and GTXDXXYG”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”
Claim · model-written summary
RcMEP2-RcMEP5 transcription rose during wheat infection.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Rhizoctonia cerealisCeratobasidium cerealet0pathogene0
- Gene
- RcMEP2-RcMEP5e0
- Outcome
- Transcription levelse0
- Direction
- markedly elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcription levels of RcMEP2-RcMEP5 markedly elevated during the fungus infection to wheat”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”
Claim · model-written summary
RcMEP2 had the highest expression.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- Rhizoctonia cerealisCeratobasidium cerealet0pathogene0
- Gene
- RcMEP2e0
- Outcome
- RcMEP2 expressede0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“among which RcMEP2 expressed with the highest level”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”
Claim · model-written summary
RcMEP2 and its M35 domain caused excess H2O2 accumulation.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- Rhizoctonia cerealisCeratobasidium cerealet0pathogene0
- Gene
- RcMEP2e0
- Outcome
- H2O2 rapidly-excessive accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RcMEP2 and its M35 domain could trigger H2O2 rapidly-excessive accumulation”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”
Claim · model-written summary
RcMEP2 and its M35 domain induced cell death.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- Rhizoctonia cerealisCeratobasidium cerealet0pathogene0
- Gene
- RcMEP2e0
- Outcome
- cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RcMEP2 and its M35 domain could trigger H2O2 rapidly-excessive accumulation, induce cell death”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”
Claim · model-written summary
RcMEP2 and its M35 domain inhibited host chitinase expression.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- Rhizoctonia cerealisCeratobasidium cerealet0pathogene0
- Gene
- RcMEP2e0
- Outcome
- expression of host chitinasese0
- Direction
- inhibite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RcMEP2 and its M35 domain could trigger H2O2 rapidly-excessive accumulation, induce cell death, and inhibit expression of host chitinases”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”
Claim · model-written summary
RcMEP2 enhanced wheat susceptibility to R. cerealis.
- Taxon
- wheatTriticum aestivume0
- Interactor
- R. cerealisCeratobasidium cerealee0pathogen
- Gene
- RcMEP2t0
- Outcome
- susceptibility of wheate0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This consequently enhanced the susceptibility of wheat to R. cerealis”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”
Claim · model-written summary
RcMEP2 signal peptide was required for secretion and cell death induction.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- Rhizoctonia cerealisCeratobasidium cerealet0pathogene0
- Gene
- RcMEP2e0
- Outcome
- secretion and cell death-inductione0
- Qualifier
- signal peptidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the predicated signal peptide of RcMEP2 functions required for secretion and cell death-induction”
t0“Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat”