Abstract
Viral proteins often interact with multiple host proteins during virus accumulation and spread. Identities and functions of all interacting host proteins are not known. Through a yeast two-hybrid screen an Arabidopsis thaliana Qa-SNARE protein [syntaxin of plants 23 (AtSYP23)], associated with pre-vacuolar compartment and vacuolar membrane fusion activities, interacted with Tobacco mosaic virus (TMV) 126 kDa protein, associated with virus accumulation and spread. In planta, AtSYP23 and AtSYP22 each fused with mCherry, co-localized with 126 kDa protein-GFP. Additionally, A. thaliana and Nicotiana benthamiana SYP2 proteins and 126 kDa protein interacted during bimolecular fluorescence complementation analysis. Decreased TMV accumulation in Arabidopsis plants lacking SYP23 and in N. benthamiana plants subjected to virus-induced gene silencing (VIGS) of SYP2 orthologs was observed. Diminished TMV accumulation during VIGS correlated with less intercellular virus spread. The inability to eliminate virus accumulation suggests that SYP2 proteins function redundantly for TMV accumulation, as for plant development.
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Claims 8
Claim · model-written summary
AtSYP23 interacted with the TMV 126 kDa protein in a yeast two-hybrid screen.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Interactor
- Tobacco mosaic virus (TMV)
Tobacco mosaic viruse1pathogen - Gene
- AtSYP23e1
- Outcome
- interacted withe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“an Arabidopsis thaliana Qa-SNARE protein [syntaxin of plants 23 (AtSYP23)], associated with pre-vacuolar compartment and vacuolar membrane fusion activities, interacted with Tobacco mosaic virus (TMV) 126 kDa protein”
Claim · model-written summary
AtSYP23 and AtSYP22 co-localize with the TMV 126 kDa protein-GFP in planta.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- AtSYP23 and AtSYP22e2
- Outcome
- co-localized with 126 kDa protein-GFPe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“an Arabidopsis thaliana Qa-SNARE protein [syntaxin of plants 23 (AtSYP23)], associated with pre-vacuolar compartment and vacuolar membrane fusion activities, interacted with Tobacco mosaic virus (TMV) 126 kDa protein”
e2“In planta, AtSYP23 and AtSYP22 each fused with mCherry, co-localized with 126 kDa protein-GFP”
Claim · model-written summary
Arabidopsis SYP2 proteins interacted with the 126 kDa protein in bimolecular fluorescence complementation.
- Taxon
- A. thalianaArabidopsis thalianae3
- Gene
- SYP2e3
- Outcome
- interactede3
- Qualifier
- bimolecular fluorescence complementation analysise3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“A. thaliana and Nicotiana benthamiana SYP2 proteins and 126 kDa protein interacted during bimolecular fluorescence complementation analysis”
Claim · model-written summary
Nicotiana benthamiana SYP2 proteins interacted with the 126 kDa protein in bimolecular fluorescence complementation.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae3 - Gene
- SYP2e3
- Outcome
- interactede3
- Qualifier
- bimolecular fluorescence complementation analysise3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“A. thaliana and Nicotiana benthamiana SYP2 proteins and 126 kDa protein interacted during bimolecular fluorescence complementation analysis”
Claim · model-written summary
TMV accumulation decreased in Arabidopsis plants lacking SYP23.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- lacking SYP23e4
- Interactor
- TMVTobacco mosaic viruse4pathogen
- Outcome
- TMV accumulatione4
- Direction
- Decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Decreased TMV accumulation in Arabidopsis plants lacking SYP23 and in N. benthamiana plants subjected to virus-induced gene silencing (VIGS) of SYP2 orthologs”
Claim · model-written summary
TMV accumulation decreased in N. benthamiana plants after VIGS of SYP2 orthologs.
- Taxon
- N. benthamianaNicotiana benthamianae4
- Treatment
- virus-induced gene silencing (VIGS) of SYP2 orthologse4
- Interactor
- TMVTobacco mosaic viruse4pathogen
- Outcome
- TMV accumulatione4
- Direction
- Decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Decreased TMV accumulation in Arabidopsis plants lacking SYP23 and in N. benthamiana plants subjected to virus-induced gene silencing (VIGS) of SYP2 orthologs”
Claim · model-written summary
Reduced TMV accumulation during VIGS correlated with less intercellular virus spread.
- Taxon
- N. benthamianaNicotiana benthamianae4
- Treatment
- VIGSe5
- Outcome
- intercellular virus spreade5
- Direction
- lesse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Decreased TMV accumulation in Arabidopsis plants lacking SYP23 and in N. benthamiana plants subjected to virus-induced gene silencing (VIGS) of SYP2 orthologs”
e5“Diminished TMV accumulation during VIGS correlated with less intercellular virus spread”
Claim · model-written summary
SYP2 proteins are suggested to function redundantly for TMV accumulation and plant development.
Verbatim
e6“The inability to eliminate virus accumulation suggests that SYP2 proteins function redundantly for TMV accumulation, as for plant development”
The same questions in other papers
loss of function · Tobacco mosaic virus · Nicotiana benthamianaPositive and negative results
7 papers in the same direction as this paper · 13 in the opposite direction
- negative
- positive
- negative
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.