Abstract
The exchange of small RNAs (sRNAs) between hosts and pathogens can lead to gene silencing in the recipient organism, a mechanism termed cross-kingdom RNAi (ck-RNAi). While fungal sRNAs promoting virulence are established, the significance of ck-RNAi in distinct plant pathogens is not clear. Here, we describe that sRNAs of the pathogen Hyaloperonospora arabidopsidis, which represents the kingdom of oomycetes and is phylogenetically distant from fungi, employ the host plant’s Argonaute (AGO)/RNA-induced silencing complex for virulence. To demonstrate H. arabidopsidis sRNA (HpasRNA) functionality in ck-RNAi, we designed a novel CRISPR endoribonuclease Csy4/GUS reporter that enabled in situ visualization of HpasRNA-induced target suppression in Arabidopsis. The significant role of HpasRNAs together with AtAGO1 in virulence was revealed in plant atago1 mutants and by transgenic Arabidopsis expressing a short-tandem-target-mimic to block HpasRNAs, that both exhibited enhanced resistance. HpasRNA-targeted plant genes contributed to host immunity, as Arabidopsis gene knockout mutants displayed quantitatively enhanced susceptibility.
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Claims 6
Claim · model-written summary
Hyaloperonospora arabidopsidis sRNAs use the host AGO silencing complex for virulence.
- Taxon
- Hyaloperonospora arabidopsidis
Hyaloperonospora arabidopsidise0 - Interactor
- host plante0host
- Gene
- Argonaute (AGO)e0
- Outcome
- virulencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sRNAs of the pathogen Hyaloperonospora arabidopsidis, which represents the kingdom of oomycetes and is phylogenetically distant from fungi, employ the host plant’s Argonaute (AGO)/RNA-induced silencing complex for virulence.”
Claim · model-written summary
A Csy4/GUS reporter enabled visualization of HpasRNA induced target suppression in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- HpasRNAe0
- Outcome
- in situ visualization of HpasRNA-induced target suppressione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel CRISPR endoribonuclease Csy4/GUS reporter that enabled in situ visualization of HpasRNA-induced target suppression in Arabidopsis.”
Claim · model-written summary
Plant atago1 mutants showed enhanced resistance.
Verbatim
e0“plant atago1 mutants and by transgenic Arabidopsis expressing a short-tandem-target-mimic to block HpasRNAs, that both exhibited enhanced resistance.”
Claim · model-written summary
Transgenic Arabidopsis expressing a HpasRNA blocking target mimic showed enhanced resistance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- expressing a short-tandem-target-mimic to block HpasRNAse0
- Interactor
- HpasRNAse0pathogen
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant atago1 mutants and by transgenic Arabidopsis expressing a short-tandem-target-mimic to block HpasRNAs, that both exhibited enhanced resistance.”
Claim · model-written summary
HpasRNA targeted plant genes contributed to host immunity.
Verbatim
e0“HpasRNA-targeted plant genes contributed to host immunity, as Arabidopsis gene knockout mutants displayed quantitatively enhanced susceptibility.”
Claim · model-written summary
Arabidopsis gene knockout mutants showed quantitatively enhanced susceptibility.
- Taxon
- ArabidopsisArabidopsise0
- Material
- gene knockout mutantse0
- Outcome
- susceptibilitye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HpasRNA-targeted plant genes contributed to host immunity, as Arabidopsis gene knockout mutants displayed quantitatively enhanced susceptibility.”