Abstract
Cucumber is dioecious by nature, having both male and female flowers, and is a model system for unisexual flower development. Knowledge related to male flowering is limited, but it is reported to be regulated by transcription factors and hormone signals. Here, we report functional characterization of the cucumber (Cucumis sativus) GL2-LIKE gene, which encodes a homeodomain leucine zipper (HD-ZIP) IV transcription factor that plays an important role in regulating male flower development. Spatial-temporal expression analyses revealed high-level expression of CsGL2-LIKE in the male flower buds and anthers. CsGL2-LIKE is closely related to AtGL2, which is known to play a key role in trichome development. However, ectopic expression of CsGL2-LIKE in Arabidopsis gl2-8 mutant was unable to rescue the gl2-8 phenotype. Interestingly, the silencing of CsGL2-LIKE delayed male flowering by inhibiting the expression of the florigen gene FT and reduced pollen vigor and seed viability. Protein-protein interaction assays showed that CsGL2-LIKE interacts with the jasmonate ZIM domain protein CsJAZ1 to form a HD-ZIP IV-CsJAZ1 complex. Collectively, our study indicates that CsGL2-LIKE regulates male flowering in cucumber, and reveals a novel function of a HD-ZIP IV transcription factor in regulating male flower development of cucumber.
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Claims 8
Claim · model-written summary
CsGL2-LIKE was highly expressed in male flower buds and anthers.
- Taxon
- cucumber
Cucumis sativust0 - Organ / stage
- male flower buds and antherse0
- Gene
- CsGL2-LIKEe0
- Outcome
- high-level expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cucumber”
e0“high-level expression of CsGL2-LIKE in the male flower buds and anthers”
Claim · model-written summary
CsGL2-LIKE is closely related to AtGL2.
- Taxon
- cucumberCucumis sativust0
- Gene
- CsGL2-LIKEe0
- Outcome
- closely related to AtGL2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cucumber”
e0“CsGL2-LIKE is closely related to AtGL2”
Claim · model-written summary
Ectopic CsGL2-LIKE expression did not rescue the Arabidopsis gl2-8 phenotype.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- gl2-8 mutante0
- Treatment
- ectopic expression of CsGL2-LIKEe0
- Gene
- CsGL2-LIKEe0
- Outcome
- gl2-8 phenotypee0
- Direction
- unable to rescuee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of CsGL2-LIKE in Arabidopsis gl2-8 mutant was unable to rescue the gl2-8 phenotype”
Claim · model-written summary
CsGL2-LIKE silencing delayed male flowering.
- Taxon
- cucumberCucumis sativust0
- Treatment
- silencing of CsGL2-LIKEe0
- Gene
- CsGL2-LIKEe0
- Outcome
- male floweringe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cucumber”
e0“silencing of CsGL2-LIKE delayed male flowering by inhibiting the expression of the florigen gene FT”
Claim · model-written summary
CsGL2-LIKE silencing inhibited FT expression.
- Taxon
- cucumberCucumis sativust0
- Treatment
- silencing of CsGL2-LIKEe0
- Gene
- FTe0
- Outcome
- expression of the florigen gene FTe0
- Direction
- inhibitinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cucumber”
e0“silencing of CsGL2-LIKE delayed male flowering by inhibiting the expression of the florigen gene FT”
Claim · model-written summary
CsGL2-LIKE silencing reduced pollen vigor.
- Taxon
- cucumberCucumis sativust0
- Treatment
- silencing of CsGL2-LIKEe0
- Gene
- CsGL2-LIKEe0
- Outcome
- pollen vigore0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cucumber”
e0“silencing of CsGL2-LIKE delayed male flowering by inhibiting the expression of the florigen gene FT and reduced pollen vigor and seed viability”
Claim · model-written summary
CsGL2-LIKE silencing reduced seed viability.
- Taxon
- cucumberCucumis sativust0
- Treatment
- silencing of CsGL2-LIKEe0
- Gene
- CsGL2-LIKEe0
- Outcome
- seed viabilitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cucumber”
e0“silencing of CsGL2-LIKE delayed male flowering by inhibiting the expression of the florigen gene FT and reduced pollen vigor and seed viability”
Claim · model-written summary
CsGL2-LIKE interacts with CsJAZ1 to form a complex.
- Taxon
- cucumberCucumis sativust0
- Gene
- CsGL2-LIKEe0
- Outcome
- interacts with the jasmonate ZIM domain protein CsJAZ1 to form a HD-ZIP IV-CsJAZ1 complexe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cucumber”
e0“CsGL2-LIKE interacts with the jasmonate ZIM domain protein CsJAZ1 to form a HD-ZIP IV-CsJAZ1 complex”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.