Abstract
The recalcitrant nature of black gram is the major constraint of in vitro regeneration and agrobacterium- mediated genetic transformation, to overcome this, a productive shoot regeneration protocol has been achieved in black gram cultivar T9 using 7-day old cotyledonary node explants excised from in vitro- raised black gram seedlings using meta-topolin. An aromatic cytokinin,meta-topolin along with BA (1.5+0.5 mg/L) in 0.50 strength MS medium with 1.5% (w/v) sucrose exhibited a maximum number of multiple shoots (32.0±0.37 shoots/explant) at the end of 6 weeks of culture. The shoots were elongated in (6.40±0.50 cm/shoot) in MS medium supplemented with GA 3 (2.0 mg/L). A maximum number of roots (9.60±0.50/shoot) and root length (11.20±0.73 cm/shoot) were obtained in combination with β-cyclodextrin(a cyclic oligosaccharide;1.5 mg/L) and IBA (1.5 mg/L). The rooted plantlets were hardened and acclimatized with least mortality rate of 2% in pot mixture consisting red soil:sand:farm yard manure (FYM) (2:1:1) and grown in green house with 85% relative humidity. Ploidy levels were analyzed using flow cytometry which confessed the chromosomal stability in invitro raised plants similar to parent plants. This protocol may be useful for producing transgenic black gram with desirable agro-traits in Indian cultivars.
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Claims 6
Claim · model-written summary
Meta-topolin with BA yielded a maximum of 32.0±0.37 shoots per explant after six weeks.
- Taxon
- black gram
Vigna mungot0 - Material
- T9e1
- Treatment
- meta-topolin along with BA (1.5+0.5 mg/L)e0
- Outcome
- number of multiple shootse0
- Direction
- maximume0
- Value
- 32.0±0.37 shoots/explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“meta-topolin along with BA (1.5+0.5 mg/L) in 0.50 strength MS medium with 1.5% (w/v) sucrose exhibited a maximum number of multiple shoots (32.0±0.37 shoots/explant) at the end of 6 weeks of culture”
t0“black gram”
e1“black gram cultivar T9”
Claim · model-written summary
GA 3 medium elongated shoots to 6.40±0.50 cm per shoot.
- Taxon
- black gramVigna mungot0
- Treatment
- MS medium supplemented with GA 3 (2.0 mg/L)e0
- Outcome
- shootse0
- Direction
- elongatede0
- Value
- 6.40±0.50 cm/shoote0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shoots were elongated in (6.40±0.50 cm/shoot) in MS medium supplemented with GA 3 (2.0 mg/L)”
t0“black gram”
Claim · model-written summary
β-cyclodextrin and IBA yielded a maximum of 9.60±0.50 roots per shoot.
- Taxon
- black gramVigna mungot0
- Treatment
- β-cyclodextrin(a cyclic oligosaccharide;1.5 mg/L) and IBA (1.5 mg/L)e0
- Outcome
- number of rootse0
- Direction
- maximume0
- Value
- 9.60±0.50/shoote0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A maximum number of roots (9.60±0.50/shoot) and root length (11.20±0.73 cm/shoot) were obtained in combination with β-cyclodextrin(a cyclic oligosaccharide;1.5 mg/L) and IBA (1.5 mg/L)”
t0“black gram”
Claim · model-written summary
β-cyclodextrin and IBA yielded root length of 11.20±0.73 cm per shoot.
- Taxon
- black gramVigna mungot0
- Treatment
- β-cyclodextrin(a cyclic oligosaccharide;1.5 mg/L) and IBA (1.5 mg/L)e0
- Outcome
- root lengthe0
- Value
- 11.20±0.73 cm/shoote0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root length (11.20±0.73 cm/shoot) were obtained in combination with β-cyclodextrin(a cyclic oligosaccharide;1.5 mg/L) and IBA (1.5 mg/L)”
t0“black gram”
Claim · model-written summary
Acclimatized plantlets had 2% mortality in the stated pot mixture and greenhouse.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rooted plantlets were hardened and acclimatized with least mortality rate of 2% in pot mixture consisting red soil:sand:farm yard manure (FYM) (2:1:1) and grown in green house with 85% relative humidity”
t0“black gram”
Claim · model-written summary
Flow cytometry found chromosomal stability similar to parent plants.
- Taxon
- black gramVigna mungot0
- Outcome
- chromosomal stabilitye0
- Comparator
- parent plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ploidy levels were analyzed using flow cytometry which confessed the chromosomal stability in invitro raised plants similar to parent plants”
t0“black gram”
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