Abstract
Studies on effect of different mutagens were carried out with three concentrations of EMS (0.2, 0.4 and 0.6%) and DMS (0.02, 0.04 and 0.06%) along with growth regulators viz., indol acetic acid and gibberellic acid (3x10−3 M) on blackgram cultivar Dungla. A dose dependent reduction in germination, seedling height and seedling dry weight was observed with increasing concentrations of mutagens. The mutagenic effect was neutralized by the GA and IAA treatment and increased the germination, seedling height and seedling dry weight, respectively. Induced pollen sterility was also significant and dose dependent at all the concentrations of mutagens and improvement in the pollen fertility was observed with post treatment of IAA and GA. A wide spectrum of chlorophyll and macro mutations was observed in the progenies of M2 generation. The frequency and spectrum of chlorophyll and macro mutants increased with increasing concentrations of the mutagens. DMS induced a broader spectrum of chlorophyll and macro mutations in M2 generation as compared to EMS. In the present investigation, DMS was more effective and EMS was efficient mutagen. Though effectiveness and efficiency computed by different methods varied in quantum, their relative proportion were comparable. A single method of studying mutagenic effectiveness and efficiency should be appropriate.
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Claims 11
Claim · model-written summary
Higher mutagen concentrations reduced germination, seedling height and dry weight.
- Taxon
- blackgram
Vigna mungot0 - Treatment
- increasing concentrations of mutagense0
- Outcome
- germination, seedling height and seedling dry weighte0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A dose dependent reduction in germination, seedling height and seedling dry weight was observed with increasing concentrations of mutagens.”
t0“blackgram”
Claim · model-written summary
GA and IAA post treatment increased germination, seedling height and dry weight.
- Taxon
- blackgramVigna mungot0
- Treatment
- GA and IAA treatmente0
- Outcome
- germination, seedling height and seedling dry weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutagenic effect was neutralized by the GA and IAA treatment and increased the germination, seedling height and seedling dry weight, respectively.”
t0“blackgram”
Claim · model-written summary
Mutagen induced pollen sterility increased with dose and was significant.
- Taxon
- blackgramVigna mungot0
- Treatment
- concentrations of mutagense0
- Outcome
- pollen sterilitye0
- Direction
- dose dependente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Induced pollen sterility was also significant and dose dependent at all the concentrations of mutagens”
t0“blackgram”
Claim · model-written summary
IAA and GA post treatment improved pollen fertility.
- Taxon
- blackgramVigna mungot0
- Treatment
- post treatment of IAA and GAe0
- Outcome
- pollen fertilitye0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“improvement in the pollen fertility was observed with post treatment of IAA and GA.”
t0“blackgram”
Claim · model-written summary
M2 progenies showed a wide spectrum of chlorophyll and macro mutations.
- Taxon
- blackgramVigna mungot0
- Organ / stage
- M2 generatione0
- Outcome
- wide spectrum of chlorophyll and macro mutationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A wide spectrum of chlorophyll and macro mutations was observed in the progenies of M2 generation.”
t0“blackgram”
Claim · model-written summary
Chlorophyll and macro mutant frequency and spectrum increased with mutagen concentration.
- Taxon
- blackgramVigna mungot0
- Treatment
- increasing concentrations of the mutagense0
- Outcome
- frequency and spectrum of chlorophyll and macro mutantse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The frequency and spectrum of chlorophyll and macro mutants increased with increasing concentrations of the mutagens.”
t0“blackgram”
Claim · model-written summary
DMS induced a broader mutation spectrum than EMS.
- Taxon
- blackgramVigna mungot0
- Treatment
- DMSe0
- Outcome
- spectrum of chlorophyll and macro mutationse0
- Direction
- broadere0
- Comparator
- EMSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DMS induced a broader spectrum of chlorophyll and macro mutations in M2 generation as compared to EMS.”
t0“blackgram”
Claim · model-written summary
DMS was more effective as a mutagen.
- Taxon
- blackgramVigna mungot0
- Treatment
- DMSe0
- Outcome
- more effectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DMS was more effective and EMS was efficient mutagen.”
t0“blackgram”
Claim · model-written summary
EMS was an efficient mutagen.
- Taxon
- blackgramVigna mungot0
- Treatment
- EMSe0
- Outcome
- efficient mutagene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DMS was more effective and EMS was efficient mutagen.”
t0“blackgram”
Claim · model-written summary
Different effectiveness and efficiency methods varied in magnitude but had comparable relative proportions.
Verbatim
e0“Though effectiveness and efficiency computed by different methods varied in quantum, their relative proportion were comparable.”
Claim · model-written summary
The authors recommended a single method to study mutagenic effectiveness and efficiency.
- Taxon
- blackgramVigna mungot0
- Outcome
- A single method of studying mutagenic effectiveness and efficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A single method of studying mutagenic effectiveness and efficiency should be appropriate.”
t0“blackgram”
The same questions in other papers
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.