Abstract
Two low-phytate soybean (Glycine max (L.) Merr.) mutant lines- V99-5089 (mips mutation on chromosome 11) and CX-1834 (mrp-l and mrp-n mutations on chromosomes 19 and 3, respectively) have proven to be valuable resources for breeding of low-phytate, high-sucrose, and low-raffinosaccharide soybeans, traits that are highly desirable from a nutritional and environmental standpoint. A recombinant inbred population derived from the cross CX1834 x V99-5089 provides an opportunity to study the effect of different combinations of these three mutations on soybean phytate and oligosaccharides levels. Of the 173 recombinant inbred lines tested, 163 lines were homozygous for various combinations of MIPS and two MRP loci alleles. These individuals were grouped into eight genotypic classes based on the combination of SNP alleles at the three mutant loci. The two genotypic classes that were homozygous mrp-l/mrp-n and either homozygous wild-type or mutant at the mips locus (MIPS/mrp-l/mrp-n or mips/mrp-l/mrp-n) displayed relatively similar ~55% reductions in seed phytate, 6.94 mg g -1 and 6.70 mg g-1 respectively, as compared with 15.2 mg g-1 in the wild-type MIPS/MRP-L/MRP-N seed. Therefore, in the presence of the double mutant mrp-l/mrp-n, the mips mutation did not cause a substantially greater decrease in seed phytate level. However, the nutritionally-desirable high-sucrose/low-stachyose/low-raffinose seed phenotype originally observed in soybeans homozygous for the mips allele was reversed in the presence of mrp-l/mrp-n mutations: homozygous mips/mrp-l/mrp-n seed displayed low-sucrose (7.70%), high-stachyose (4.18%), and the highest observed raffinose (0.94%) contents per gram of dry seed. Perhaps the block in phytic acid transport from its cytoplasmic synthesis site to its storage site, conditioned by mrp-l/mrp-n, alters myo-inositol flux in mips seeds in a way that restores to wild-type levels the mips conditioned reductions in raffinosaccharides. Overall this study determined the combinatorial effects of three low phytic acid causing mutations on regulation of seed phytate and oligosaccharides in soybean.
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Claims 7
Claim · model-written summary
Homozygous mrp-l/mrp-n seed with wild-type MIPS showed about 55% lower seed phytate (6.94 mg g-1) than wild-type MIPS/MRP-L/MRP-N seed.
- Taxon
- soybean
Glycine maxt0 - Material
- MIPS/mrp-l/mrp-ne0
- Outcome
- seed phytatee0
- Direction
- reductionse0
- Value
- ~55%e0
- Qualifier
- 6.94 mg g -1e0
- Comparator
- wild-type MIPS/MRP-L/MRP-N seede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean”
e0“homozygous wild-type or mutant at the mips locus (MIPS/mrp-l/mrp-n or mips/mrp-l/mrp-n) displayed relatively similar ~55% reductions in seed phytate, 6.94 mg g -1 and 6.70 mg g-1 respectively, as compared with 15.2 mg g-1 in the wild-type MIPS/MRP-L/MRP-N seed”
Claim · model-written summary
Homozygous mips/mrp-l/mrp-n seed showed about 55% lower seed phytate (6.70 mg g-1) than wild-type MIPS/MRP-L/MRP-N seed.
- Taxon
- soybeanGlycine maxt0
- Material
- mips/mrp-l/mrp-ne0
- Outcome
- seed phytatee0
- Direction
- reductionse0
- Value
- ~55%e0
- Qualifier
- 6.70 mg g-1e0
- Comparator
- wild-type MIPS/MRP-L/MRP-N seede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean”
e0“homozygous wild-type or mutant at the mips locus (MIPS/mrp-l/mrp-n or mips/mrp-l/mrp-n) displayed relatively similar ~55% reductions in seed phytate, 6.94 mg g -1 and 6.70 mg g-1 respectively, as compared with 15.2 mg g-1 in the wild-type MIPS/MRP-L/MRP-N seed”
Claim · model-written summary
In the presence of the double mutant mrp-l/mrp-n, the mips mutation did not cause a substantially greater decrease in seed phytate level.
- Taxon
- soybeanGlycine maxt0
- Treatment
- mips mutatione1
- Outcome
- seed phytate levele1
- Direction
- substantially greater decreasee1
- Qualifier
- in the presence of the double mutant mrp-l/mrp-ne1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean”
e1“in the presence of the double mutant mrp-l/mrp-n, the mips mutation did not cause a substantially greater decrease in seed phytate level”
Claim · model-written summary
The high-sucrose/low-stachyose/low-raffinose seed phenotype of mips homozygous soybean was reversed in the presence of mrp-l/mrp-n mutations.
- Taxon
- soybeanGlycine maxt0
- Treatment
- mrp-l/mrp-n mutationse2
- Outcome
- high-sucrose/low-stachyose/low-raffinose seed phenotypee2
- Direction
- reversede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean”
e2“the nutritionally-desirable high-sucrose/low-stachyose/low-raffinose seed phenotype originally observed in soybeans homozygous for the mips allele was reversed in the presence of mrp-l/mrp-n mutations”
Claim · model-written summary
Seed of homozygous mips/mrp-l/mrp-n soybean had low sucrose (7.70%).
- Taxon
- soybeanGlycine maxt0
- Material
- homozygous mips/mrp-l/mrp-ne3
- Outcome
- sucrosee3
- Direction
- low-sucrosee3
- Value
- 7.70%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean”
e3“homozygous mips/mrp-l/mrp-n seed displayed low-sucrose (7.70%), high-stachyose (4.18%), and the highest observed raffinose (0.94%) contents per gram of dry seed”
Claim · model-written summary
Seed of homozygous mips/mrp-l/mrp-n soybean had high stachyose (4.18%).
- Taxon
- soybeanGlycine maxt0
- Material
- homozygous mips/mrp-l/mrp-ne3
- Outcome
- stachyosee3
- Direction
- high-stachyosee3
- Value
- 4.18%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean”
e3“homozygous mips/mrp-l/mrp-n seed displayed low-sucrose (7.70%), high-stachyose (4.18%), and the highest observed raffinose (0.94%) contents per gram of dry seed”
Claim · model-written summary
Seed of homozygous mips/mrp-l/mrp-n soybean had the highest observed raffinose content (0.94%).
- Taxon
- soybeanGlycine maxt0
- Material
- homozygous mips/mrp-l/mrp-ne3
- Outcome
- raffinosee3
- Direction
- higheste3
- Value
- 0.94%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean”
e3“homozygous mips/mrp-l/mrp-n seed displayed low-sucrose (7.70%), high-stachyose (4.18%), and the highest observed raffinose (0.94%) contents per gram of dry seed”