Abstract
Micropropagation is a technique that allows to obtain quality plants, making it an important tool in the production of grapevine matrices. Composition of the culture media and plant growth regulators are commonly tested to adapt micropropagation protocols. The objective of this study was to test concentrations of Murashige & Skoog (MS) culture media and BAP (6-benzylaminopurine) and IBA (indolbutyric acid) plant growth regulators in the multiplication and in vitro rooting stages as part of the elaboration of a Chardonnay grapevine micropropagation protocol. In both experiments, multiplication and rooting, the experimental design was completely randomized, with a bifactorial scheme, testing culture media (MS and MS/2) and BAP concentrations (0; 5; 10 μM) in the multiplication and of IBA (0; 0.5; 1.0; 1.5 μM) in rooting. For multiplication, the best results for number of buds, leaves and shoots were obtained with 10 μM BAP, and the concentrations of the culture media had no significant effect for most of the variables. For rooting, the MS media provided better results. In relation to IBA, the number and average root length were influenced by the concentration used, with 0.5 and 1.0 μM providing the best results. Grapevine explants may be multiplied in MS/2 media supplemented with 10 μM BAP and subsequently rooted in MS media with 0.5 μM IBA.Index terms: Vitis vinifera; tissue culture; micropropagation; cytokinin; auxin.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
In multiplication, 10 μM BAP gave the best results for number of buds, leaves and shoots of grapevine.
- Treatment
- 10 μM BAPe1
- Outcome
- number of buds, leaves and shootse1
- Direction
- best resultse1
- Setting
- In vitrot0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro multiplication and rooting of grapevine”
e1“For multiplication, the best results for number of buds, leaves and shoots were obtained with 10 μM BAP”
Claim · model-written summary
Culture media concentrations (MS and MS/2) had no significant effect on most multiplication variables of grapevine.
- Treatment
- culture media (MS and MS/2)e3
- Outcome
- most of the variablese2
- Direction
- no significant effecte2
- Qualifier
- for most of the variablese2
- Setting
- In vitrot0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro multiplication and rooting of grapevine”
e2“the concentrations of the culture media had no significant effect for most of the variables”
e3“testing culture media (MS and MS/2)”
Claim · model-written summary
MS media gave better rooting results than the other medium tested for grapevine.
- Treatment
- MS mediae4
- Outcome
- better resultse4
- Direction
- better resultse4
- Qualifier
- rootinge4
- Setting
- In vitrot0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro multiplication and rooting of grapevine”
e4“For rooting, the MS media provided better results”
Claim · model-written summary
IBA at 0.5 and 1.0 μM gave the best number of roots and average root length of grapevine.
- Treatment
- IBAe6
- Outcome
- number and average root lengthe5
- Direction
- best resultse5
- Qualifier
- 0.5 and 1.0 μMe5
- Setting
- In vitrot0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro multiplication and rooting of grapevine”
e5“the number and average root length were influenced by the concentration used, with 0.5 and 1.0 μM providing the best results”
e6“In relation to IBA”
Claim · model-written summary
Grapevine explants may be multiplied in MS/2 media with 10 μM BAP and then rooted in MS media with 0.5 μM IBA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Grapevine explants may be multiplied in MS/2 media supplemented with 10 μM BAP and subsequently rooted in MS media with 0.5 μM IBA”
The same questions in other papers
indole-3-butyric acid · growth · VitisResults differ
32 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
30 papers more in this group
See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.