Abstract
In-ovulo embryo rescue in grape breeding programme assures breeding efficiency by curtailing 6 to 8 years in the development of seedless grape cultivars. Effect of different growth regulators, culture media, ovule age, cultivars and mycorrhizal strains on successful in-ovulo embryo rescue were studied in this experiment. With respect to mean ovule age (days after pollination), maximum ovule-embryo growth (2.13 mm2) were obtained when ovules were cultured at 24 days after pollination (DAP) but maximum germination (12.67 %) was obtained when ovules were cultured at 28 DAP. The concentration of IAA (4 mg) + GA3 (0.5 mg) proved to be most effective for germination (13.84 %). Among various media used for culturing ovules on modified MS medium (1/2 macro + 1/1 micro) required minimum days to germinate (96.67) and registered highest germination (13.75 %). Chilling treatment was proved to be one of the important factors for embryo maturation and 60 d of chilling treatment at 4 °C improved embryo germination. With respect to the effect of different growth regulators on various rooting and shooting parameters IBA (1.0 mg·L-1) and NAA (1.5 mg·L-1) were found superior. While comparing different hardening strategies, a glass jar with polypropylene cap (GPP) was found to be most effective as far as hardening success (66.67 %) was concerned. One of the serious impediments in the success of in vitro rescued plantlets is very high field mortality, field survival of rescued plantlets can be effectively increased by using different mycorrhizal strains (AMF) as bio-hardening agents. Among the various strains used for hardening of rescued plantlets T3 inoculated plantlets registered highest survival percent (88.00).
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Claims 11
Claim · model-written summary
Ovules cultured at 24 days after pollination had maximum ovule-embryo growth of 2.13 mm2.
- Taxon
- grapet0
- Organ / stage
- ovulese0
- Treatment
- 24 days after pollination (DAP)e0
- Outcome
- ovule-embryo growthe0
- Direction
- maximume0
- Value
- 2.13 mm2e0
Not reportedComparator
Verbatim
e0“maximum ovule-embryo growth (2.13 mm2) were obtained when ovules were cultured at 24 days after pollination (DAP)”
t0“grape”
Claim · model-written summary
Ovules cultured at 28 DAP had maximum germination of 12.67 %.
Not reportedComparator
Verbatim
e0“maximum germination (12.67 %) was obtained when ovules were cultured at 28 DAP”
t0“grape”
Claim · model-written summary
IAA at 4 mg plus GA3 at 0.5 mg gave the most effective germination, 13.84 %.
- Taxon
- grapet0
- Treatment
- IAA (4 mg) + GA3 (0.5 mg)e0
- Outcome
- germinatione0
- Direction
- most effectivee0
- Value
- 13.84 %e0
Not reportedComparator
Verbatim
e0“The concentration of IAA (4 mg) + GA3 (0.5 mg) proved to be most effective for germination (13.84 %)”
t0“grape”
Claim · model-written summary
Modified MS medium gave the minimum time to germination, 96.67 days.
- Taxon
- grapet0
- Treatment
- modified MS medium (1/2 macro + 1/1 micro)e0
- Outcome
- days to germinatee0
- Direction
- minimume0
- Value
- 96.67e0
Not reportedComparator
Verbatim
e0“modified MS medium (1/2 macro + 1/1 micro) required minimum days to germinate (96.67)”
t0“grape”
Claim · model-written summary
Modified MS medium gave the highest germination, 13.75 %.
- Taxon
- grapet0
- Treatment
- modified MS medium (1/2 macro + 1/1 micro)e0
- Outcome
- germinatione0
- Direction
- higheste0
- Value
- 13.75 %e0
Not reportedComparator
Verbatim
e0“modified MS medium (1/2 macro + 1/1 micro) required minimum days to germinate (96.67) and registered highest germination (13.75 %)”
t0“grape”
Claim · model-written summary
Chilling for 60 days at 4 °C improved embryo germination.
- Taxon
- grapet0
- Treatment
- 60 d of chilling treatment at 4 °Ce0
- Outcome
- embryo germinatione0
- Direction
- improvede0
Not reportedComparator
Verbatim
e0“60 d of chilling treatment at 4 °C improved embryo germination”
t0“grape”
Claim · model-written summary
IBA at 1.0 mg·L-1 was superior for rooting and shooting parameters.
Not reportedComparator
Verbatim
e0“IBA (1.0 mg·L-1) and NAA (1.5 mg·L-1) were found superior”
e1“rooting and shooting parameters”
t0“grape”
Claim · model-written summary
NAA at 1.5 mg·L-1 was superior for rooting and shooting parameters.
Not reportedComparator
Verbatim
e0“IBA (1.0 mg·L-1) and NAA (1.5 mg·L-1) were found superior”
e1“rooting and shooting parameters”
t0“grape”
Claim · model-written summary
A glass jar with polypropylene cap gave the highest hardening success, 66.67 %.
- Taxon
- grapet0
- Treatment
- a glass jar with polypropylene cap (GPP)e0
- Outcome
- hardening successe0
- Direction
- most effectivee0
- Value
- 66.67 %e0
Not reportedComparator
Verbatim
e0“a glass jar with polypropylene cap (GPP) was found to be most effective as far as hardening success (66.67 %) was concerned”
t0“grape”
Claim · model-written summary
Mycorrhizal strains increased field survival of rescued grape plantlets.
- Taxon
- grapet0
- Treatment
- using different mycorrhizal strains (AMF)e0
- Interactor
- mycorrhizal strains (AMF)e0symbiont
- Outcome
- field survival of rescued plantletse0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“field survival of rescued plantlets can be effectively increased by using different mycorrhizal strains (AMF) as bio-hardening agents”
t0“grape”
Claim · model-written summary
T3 inoculated plantlets had the highest survival, 88.00 percent.
- Taxon
- grapet0
- Treatment
- T3 inoculatede0
- Interactor
- mycorrhizal strains (AMF)e1symbiont
- Outcome
- survival percente0
- Direction
- higheste0
- Value
- 88.00e0
Not reportedComparator
Verbatim
e0“T3 inoculated plantlets registered highest survival percent (88.00)”
e1“mycorrhizal strains (AMF)”
t0“grape”
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