Abstract
Abstract Zygotic embryos isolated from mature berries of Vitis longii Prince and V. vinifera L. cvs. Chardonnay, French Colombard, Grenache, and White Riesling produced somatic embryos when cultured on solid Nitsch and Nitsch medium. Explants of V. vinifera cv. Pinot noir did not produce somatic embryos. The highest incidence of somatic embryogenesis occurred on medium containing (all in mg·liter −1 ) 1.0 NOA plus 0.2 BAP, except in ‘White Riesling’, where 1.0 NOA plus 1.0 BAP was optimal. Somatic embryos developed asynchronously from a mass of white proembryos and passed through globular, heart, and torpedo-shaped stages. Embryos proliferated by budding at the surface of globular embryos. With regular transfers, embryogenic tissues remained morphogenically competent for up to 1 year. Zygotic embryo explants cultured on medium without growth regulators usually germinated within 10 days and produced healthy seedlings. About 40% of somatic embryos germinated after isolation from their parental cultures and transfer to fresh medium. At the concentrations tested, NAA, BAP, and GA 3 did not improve germination frequency over that obtained with basal medium and, in some cases, severely reduced germination. Plants were established in the greenhouse and no morphological abnormalities were observed. Chemical names used: 6-benzylam-inopurine (BAP); gibberellic acid (GA 3 ); 2-(l-naphthyl)acetic acid (NAA); 2-naphthoxyacetic acid (NOA).
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Claims 11
Claim · model-written summary
Embryos from the named Vitis cultivars produced somatic embryos on solid medium.
- Taxon
- Vitis longii Prince
Vitis longiie0 - Organ / stage
- Zygotic embryose0
- Treatment
- solid Nitsch and Nitsch mediume0
- Outcome
- produced somatic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zygotic embryos isolated from mature berries of Vitis longii Prince and V. vinifera L. cvs. Chardonnay, French Colombard, Grenache, and White Riesling produced somatic embryos when cultured on solid Nitsch and Nitsch medium.”
Claim · model-written summary
Pinot noir explants did not produce somatic embryos.
- Taxon
- V. vinifera
Vitis viniferae0 - Material
- Pinot noire0
- Outcome
- did not produce somatic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Explants of V. vinifera cv. Pinot noir did not produce somatic embryos.”
Claim · model-written summary
The highest somatic embryogenesis incidence occurred with 1.0 NOA plus 0.2 BAP.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest incidence of somatic embryogenesis occurred on medium containing (all in mg·liter −1 ) 1.0 NOA plus 0.2 BAP, except in ‘White Riesling’, where 1.0 NOA plus 1.0 BAP was optimal.”
t0“Grapevine”
Claim · model-written summary
White Riesling responded optimally to 1.0 NOA plus 1.0 BAP.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“except in ‘White Riesling’, where 1.0 NOA plus 1.0 BAP was optimal.”
t0“Grapevine”
Claim · model-written summary
Somatic embryos developed asynchronously through globular, heart and torpedo stages.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos developed asynchronously from a mass of white proembryos and passed through globular, heart, and torpedo-shaped stages.”
t0“Grapevine”
Claim · model-written summary
Embryos proliferated by budding on globular embryo surfaces.
- Outcome
- proliferated by budding at the surface of globular embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryos proliferated by budding at the surface of globular embryos.”
t0“Grapevine”
Claim · model-written summary
Regularly transferred embryogenic tissues retained morphogenic competence for up to a year.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With regular transfers, embryogenic tissues remained morphogenically competent for up to 1 year.”
t0“Grapevine”
Claim · model-written summary
Embryo explants without growth regulators usually germinated within 10 days and produced healthy seedlings.
- Treatment
- medium without growth regulatorse0
- Outcome
- germinated within 10 days and produced healthy seedlingse0
- Value
- 10 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zygotic embryo explants cultured on medium without growth regulators usually germinated within 10 days and produced healthy seedlings.”
t0“Grapevine”
Claim · model-written summary
About 40% of isolated somatic embryos germinated after transfer.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“About 40% of somatic embryos germinated after isolation from their parental cultures and transfer to fresh medium.”
t0“Grapevine”
Claim · model-written summary
NAA, BAP and GA 3 did not improve germination and sometimes severely reduced it.
- Treatment
- NAA, BAP, and GA 3e0
- Outcome
- germination frequencye0
- Direction
- did not improvee0
- Qualifier
- in some cases, severely reduced germinatione0
- Comparator
- basal mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the concentrations tested, NAA, BAP, and GA 3 did not improve germination frequency over that obtained with basal medium and, in some cases, severely reduced germination.”
t0“Grapevine”
Claim · model-written summary
Greenhouse plants showed no morphological abnormalities.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants were established in the greenhouse and no morphological abnormalities were observed.”
t0“Grapevine”
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See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.