Abstract
ABSTRACT Hybridisation was carried out between stenospermocarpic seedless grape cultivars (female parents) and Chinese wild Vitis amurensis or its hybrids with cold‐resistance alleles (male parents). The aim of this paper was: (1) to evaluate the effects of the addition of the plant growth regulator indole‐3‐acetic acid (IAA) to the embryo development medium MM3 + 500 mg L −1 casein hydrolysate + 1 mmol L −1 serine; (2) to obtain a batch of seedless hybrid grape offspring by embryo rescue; and (3) to screen suitable genotypes for embryo rescue. The results indicated that adding 1.75 mg L −1 IAA promotes embryo development, germination and plantlet formation in the combinations of ‘Ruby Seedless’ × ‘00‐1‐5’ and ‘Beauty Seedless’ × ‘Beichun’. However, it inhibits the combinations ‘Perlette’ × ‘00‐1‐5’ and ‘Neptune’ × ‘00‐1‐5’. ‘Crimson Seedless’, ‘Ruby Seedless’ and ‘Flame Seedless’ were more suitable as female parents than ‘Neptune’, ‘Perlette’, ‘Beauty Seedless’, ‘Heshen Seedless’, ‘Jupiter’ and ‘Su‐67’. The Hybrid ‘00‐1‐5’ performed best as the male parent for cold‐resistant seedless grape embryo rescue. A total of 267 new hybrid progenies derived from 11 cross combinations were obtained by embryo rescue. GLSP1 and SCF27, two special primers of the molecular marker linked to seedless gene in grape, were employed to screen the progenies of five cross combinations for possible seedlessness. Five of 22 strains obtained from two cross combinations had the marker GLSP1‐569; 43 of 89 strains obtained from three cross combinations had the marker SCF27‐2000. These marked strains were preliminarily identified as being seedless.
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Claims 10
Claim · model-written summary
IAA promoted embryo development, germination and plantlet formation in Ruby Seedless by 00-1-5 crosses.
- Taxon
- grapest0
- Material
- ‘Ruby Seedless’ × ‘00‐1‐5’e0
- Treatment
- 1.75 mg L −1 IAAe0
- Outcome
- embryo development, germination and plantlet formatione0
- Direction
- promotese0
Not reportedComparator
Verbatim
t0“grapes”
e0“adding 1.75 mg L −1 IAA promotes embryo development, germination and plantlet formation in the combinations of ‘Ruby Seedless’ × ‘00‐1‐5’ and ‘Beauty Seedless’ × ‘Beichun’.”
Claim · model-written summary
IAA promoted embryo development, germination and plantlet formation in Beauty Seedless by Beichun crosses.
- Taxon
- grapest0
- Material
- ‘Beauty Seedless’ × ‘Beichun’e0
- Treatment
- 1.75 mg L −1 IAAe0
- Outcome
- embryo development, germination and plantlet formatione0
- Direction
- promotese0
Not reportedComparator
Verbatim
t0“grapes”
e0“adding 1.75 mg L −1 IAA promotes embryo development, germination and plantlet formation in the combinations of ‘Ruby Seedless’ × ‘00‐1‐5’ and ‘Beauty Seedless’ × ‘Beichun’.”
Claim · model-written summary
IAA inhibited Perlette by 00-1-5 embryo rescue combination.
- Taxon
- grapest0
- Material
- ‘Perlette’ × ‘00‐1‐5’e0
- Outcome
- combinations ‘Perlette’ × ‘00‐1‐5’e0
- Direction
- inhibitse0
Not reportedTreatmentComparator
Verbatim
t0“grapes”
e0“However, it inhibits the combinations ‘Perlette’ × ‘00‐1‐5’ and ‘Neptune’ × ‘00‐1‐5’.”
Claim · model-written summary
IAA inhibited Neptune by 00-1-5 embryo rescue combination.
Not reportedTreatmentComparator
Verbatim
t0“grapes”
e0“However, it inhibits the combinations ‘Perlette’ × ‘00‐1‐5’ and ‘Neptune’ × ‘00‐1‐5’.”
Claim · model-written summary
Crimson Seedless, Ruby Seedless and Flame Seedless were more suitable female parents than the listed alternatives.
Verbatim
t0“grapes”
e0“‘Crimson Seedless’, ‘Ruby Seedless’ and ‘Flame Seedless’ were more suitable as female parents than ‘Neptune’, ‘Perlette’, ‘Beauty Seedless’, ‘Heshen Seedless’, ‘Jupiter’ and ‘Su‐67’.”
Claim · model-written summary
Hybrid 00-1-5 performed best as male parent.
Verbatim
t0“grapes”
e0“The Hybrid ‘00‐1‐5’ performed best as the male parent for cold‐resistant seedless grape embryo rescue.”
Claim · model-written summary
Embryo rescue produced 267 hybrid progenies from 11 crosses.
Verbatim
t0“grapes”
e0“A total of 267 new hybrid progenies derived from 11 cross combinations were obtained by embryo rescue.”
Claim · model-written summary
GLSP1-569 marked five of 22 strains from two crosses as preliminarily seedless.
Verbatim
t0“grapes”
e0“Five of 22 strains obtained from two cross combinations had the marker GLSP1‐569”
Claim · model-written summary
SCF27-2000 marked 43 of 89 strains from three crosses as preliminarily seedless.
Verbatim
t0“grapes”
e0“43 of 89 strains obtained from three cross combinations had the marker SCF27‐2000”
Claim · model-written summary
The marked strains were preliminarily identified as seedless.
Verbatim
t0“grapes”
e0“These marked strains were preliminarily identified as being seedless.”
The same questions in other papers
indole-3-acetic acid · growth · VitisResults agree
12 papers in the same direction as this paper
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.