Abstract
(1) Aims: Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes. (2) Methods: Bacterial communities were identified through Illumina Miseq sequencing of the 16S rRNA gene amplicons, followed by culture dependent methods to isolate plant growth-promoting bacteria (PGPB). Plant growth-promoting traits of the cultivable bacterial fraction were further analyzed. To screen for the presence of nitrogen-fixing bacteria, the promiscuous tropical legume Vigna unguiculata was used as a trap host. The taxonomy of all purified isolates was genetically verified by 16S rRNA gene Sanger sequencing. (3) Results: Bacterial community results indicated that fire did not drive major changes in bacterial abundance. However, culture-dependent methods allowed the differentiation of bacterial communities between the sampled sites, which were particularly enriched in Proteobacteria with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growth. Bradyrhizobium was the most frequent symbiotic bacteria trapped in cowpea nodules coexisting with other endophytic bacteria. (4) Conclusion: Although the global analysis did not show significant differences between landscapes or sites with different fire regimes, probably due to the fast recovery of bacterial communities, the isolation of PGPB suggests that the rhizosphere bacteria are driven by the plant species, soil type, and fire regime, and are potentially associated with a wide range of agricultural, environmental, and industrial applications. Thus, the rhizosphere of African savannah ecosystems seems to be an untapped source of bacterial species and strains that should be further exploited for bio-based solutions.
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Claims 7
Claim · model-written summary
Fire regime did not drive major changes in rhizosphere bacterial abundance of the savanna trees.
- Taxon
- Colophospermum mopane and Combretum apiculatum
Colophospermum mopane
Combretum apiculatume1 - Treatment
- fire regimese1
- Outcome
- bacterial abundancee2
- Direction
- did not drive major changese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes”
e2“fire did not drive major changes in bacterial abundance”
Claim · model-written summary
Culture-dependent methods differentiated rhizosphere bacterial communities between the sampled sites.
- Taxon
- Colophospermum mopane and Combretum apiculatumColophospermum mopaneCombretum apiculatume1
- Organ / stage
- rhizospheree1
- Outcome
- differentiation of bacterial communitiese3
- Qualifier
- between the sampled sitese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes”
e3“culture-dependent methods allowed the differentiation of bacterial communities between the sampled sites, which were particularly enriched in Proteobacteria with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growth”
Claim · model-written summary
The sampled sites were enriched in Proteobacteria with a wide range of plant-beneficial traits.
- Taxon
- Colophospermum mopane and Combretum apiculatumColophospermum mopaneCombretum apiculatume1
- Organ / stage
- rhizospheree1
- Outcome
- enriched in Proteobacteriae3
- Qualifier
- with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growthe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes”
e3“culture-dependent methods allowed the differentiation of bacterial communities between the sampled sites, which were particularly enriched in Proteobacteria with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growth”
Claim · model-written summary
Bradyrhizobium was the most frequent symbiotic bacterium trapped in cowpea nodules.
- Taxon
- Vigna unguiculata
Vigna unguiculatae5 - Organ / stage
- nodulese4
- Interactor
- Bradyrhizobium
Bradyrhizobiume4symbiont - Outcome
- most frequent symbiotic bacteriae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Bradyrhizobium was the most frequent symbiotic bacteria trapped in cowpea nodules coexisting with other endophytic bacteria”
e5“the promiscuous tropical legume Vigna unguiculata was used as a trap host”
Claim · model-written summary
The global analysis showed no significant difference in rhizosphere bacteria between landscapes or sites with different fire regimes.
- Taxon
- Colophospermum mopane and Combretum apiculatumColophospermum mopaneCombretum apiculatume1
- Treatment
- different fire regimese6
- Outcome
- significant differencese6
- Direction
- did not showe6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes”
e6“the global analysis did not show significant differences between landscapes or sites with different fire regimes”
Claim · model-written summary
Rhizosphere bacteria appear to be associated with plant species, soil type and fire regime.
- Taxon
- Colophospermum mopane and Combretum apiculatumColophospermum mopaneCombretum apiculatume1
- Treatment
- plant species, soil type, and fire regimee0
- Outcome
- rhizosphere bacteriae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes”
e0“the isolation of PGPB suggests that the rhizosphere bacteria are driven by the plant species, soil type, and fire regime”
Claim · model-written summary
The rhizosphere of African savanna trees should be further exploited as a source of bacterial species and strains.
- Taxon
- Colophospermum mopane and Combretum apiculatumColophospermum mopaneCombretum apiculatume1
- Outcome
- bacterial species and strainse7
- Qualifier
- should be further exploitede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes”
e7“the rhizosphere of African savannah ecosystems seems to be an untapped source of bacterial species and strains that should be further exploited”