Abstract
The productivity of the Australian dairy industry is underpinned by pasture grasses, and importantly perennial ryegrass. The performance of these pasture grasses is supported by the fungal endophyte Epichloë spp. that has bioprotection activities, however the broader microbiome is not well characterised. In this study, we characterised a novel bioprotectant Xanthomonas species isolated from perennial ryegrass (Lolium perenne L. cv. Alto). In vitro and in planta bioassays against key fungal pathogens of grasses (Sclerotium rolfsii, Drechslera brizae and Microdochium nivale) indicated strong bioprotection activities. A complete circular chromosome of ~5.2 Mb was generated for three strains of the novel Xanthomonas sp. Based on the 16S ribosomal RNA gene, the strains were closely related to the plant pathogen Xanthomonas translucens, however comparative genomics of 22 closely related xanthomonad strains indicated that these strains were a novel species. The comparative genomics analysis also identified two unique gene clusters associated with the production of bioprotectant secondary metabolites including one associated with a novel nonribosomal peptide synthetase and another with a siderophore. The analysis also identified genes associated with an endophytic lifestyle (e.g. Type VI secretion system), while no genes associated with pathogenicity were identified (e.g. Type III secretion system and effectors). Overall, these results indicate that these strains represent a novel, bioactive, non-pathogenic species of the genus Xanthomonas. Strain GW was the designated type strain of this novel Xanthomonas sp.
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Claims 7
Claim · model-written summary
A novel Xanthomonas species isolated from perennial ryegrass showed strong bioprotection activity in vitro and in planta against the grass fungal pathogens Sclerotium rolfsii, Drechslera brizae and Microdochium nivale.
- Taxon
- perennial ryegrass
Lolium perennee1 - Treatment
- novel bioprotectant Xanthomonas speciese1
- Interactor
- Sclerotium rolfsii
Agroathelia rolfsiie0pathogen - Second interactor
- Xanthomonas
_-_Howard_F._Schwartz%2C_Colorado_State_University.jpg?width=60)
Xanthomonase1biocontrol_agent - Outcome
- bioprotection activitiese0
- Qualifier
- Drechslera brizae and Microdochium nivalee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Howard_F._Schwartz%2C_Colorado_State_University.jpg?width=500)
Verbatim
e0“In vitro and in planta bioassays against key fungal pathogens of grasses (Sclerotium rolfsii, Drechslera brizae and Microdochium nivale) indicated strong bioprotection activities”
e1“we characterised a novel bioprotectant Xanthomonas species isolated from perennial ryegrass (Lolium perenne L. cv. Alto)”
Claim · model-written summary
Comparative genomics of 22 closely related xanthomonad strains indicated that the strains were a novel species.
Not reportedDirection
Verbatim
e0“comparative genomics of 22 closely related xanthomonad strains indicated that these strains were a novel species”
Claim · model-written summary
Comparative genomics identified two unique gene clusters associated with production of bioprotectant secondary metabolites.
- Taxon
- XanthomonasXanthomonast0
- Outcome
- two unique gene clusters associated with the production of bioprotectant secondary metabolitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Novel Xanthomonas Species”
e0“The comparative genomics analysis also identified two unique gene clusters associated with the production of bioprotectant secondary metabolites”
Claim · model-written summary
Genes associated with an endophytic lifestyle, such as the Type VI secretion system, were identified in the novel Xanthomonas strains.
- Taxon
- XanthomonasXanthomonast0
- Outcome
- genes associated with an endophytic lifestylee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Novel Xanthomonas Species”
e0“The analysis also identified genes associated with an endophytic lifestyle (e.g. Type VI secretion system)”
Claim · model-written summary
No genes associated with pathogenicity, such as the Type III secretion system and effectors, were identified in the novel Xanthomonas strains.
- Taxon
- XanthomonasXanthomonast0
- Outcome
- no genes associated with pathogenicity were identifiede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Novel Xanthomonas Species”
e0“no genes associated with pathogenicity were identified (e.g. Type III secretion system and effectors)”
Claim · model-written summary
A complete circular chromosome of about 5.2 Mb was generated for three strains of the novel Xanthomonas species.
- Taxon
- XanthomonasXanthomonase0
- Outcome
- complete circular chromosomee0
- Value
- ~5.2 Mbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A complete circular chromosome of ~5.2 Mb was generated for three strains of the novel Xanthomonas sp”
Claim · model-written summary
Strain GW was designated the type strain of the novel Xanthomonas species.
- Taxon
- XanthomonasXanthomonase0
- Outcome
- designated type straine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strain GW was the designated type strain of this novel Xanthomonas sp”