Paper · 2014 · Anbar Journal of Agricultural Sciences

Effect of Sterilization methods and Replication in the Microbial community and the Growth of the Soybean plant Glycine max L. in the Gypsum soil

Abedul Kareem Erabi; Shaimaa abd mohhamed ali

6
claims
13
verbatim quotes
3
citations
2
research areas
✓
Open access · cc-by

Abstract

Pots experiment was conducted to study the effect of sterilization methods and resterilization on the microbial community and numbers and the growth of soybean plant characteristics, The results indicated that the effect of sterilization methods and replicated were significant on the number of bacteria and fungi , and on growth characteristics of soybean plant , the number of bacteria and fungi were decrease significantly before and after the sowing by the effect of Autoclave and formalin sterilization in compare with non sterilized treatment , the two time of sterilization cause additional significant decreas , the lowest number recorded at replicated formalin treatment ,The percentage of mycorrhizal infection was decrease significantly by sterilization , the replicated cause additional decrease , the mycorrhizal infection decrease significantly at formalin sterilization in compare with autoclave , the replicated formaline treatment gave the lowest value , this treatment dry soil compare with 1-CFU gm 51 x 10 whichthe number of bacteria and fungi gavefor fungi 510 x 10d for bacteria an 5sterilization treatment which gave 44 x 10 nonrespectively , as well as the mycorrhizal infection percentage of replicated formalin treatment was 20% in compared with 71.60 , 51.66 ,35 and 31.66% for control , Autoclave for one time , Autoclave replicated and formalin fore one time respectively .Also the results showed that sterilization with Autoclave and formalin cause significant decrease in germination percentage, height of soybean plant and the dry weight of shoot and root part in compare with non sterilized treatment , the results indicated that the growth characteristics value of Autoclave treatment was higher than formaline treatment , the formaline replicated treatment gave the lowest value of germination percentage hight plant , shoot and root dry weight .

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Claims 6

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Autoclave and formalin sterilization significantly decreased soybean germination percentage, plant height and shoot and root dry weight relative to non-sterilized soil.

Taxon
soybeanGlycine maxe0
Treatment
sterilization with Autoclave and formaline1
Outcome
germination percentage, height of soybean plant and the dry weight of shoot and root parte1
Direction
decreasee1
Comparator
non sterilized treatmente1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“growth characteristics of soybean plant”

✓ located verbatim in the source · abstract, characters 323–362 · show in text ↑
e1

“sterilization with Autoclave and formalin cause significant decrease in germination percentage, height of soybean plant and the dry weight of shoot and root part in compare with non sterilized treatment”

✓ located verbatim in the source · abstract, characters 1431–1633 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Autoclave and formalin sterilization significantly decreased the number of bacteria and fungi relative to non-sterilized soil.

Taxon
soybeanGlycine maxe0
Treatment
Autoclave and formalin sterilizatione2
Outcome
number of bacteria and fungie2
Direction
decreasee2
Comparator
non sterilized treatmente2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“growth characteristics of soybean plant”

✓ located verbatim in the source · abstract, characters 323–362 · show in text ↑
e2

“the number of bacteria and fungi were decrease significantly before and after the sowing by the effect of Autoclave and formalin sterilization in compare with non sterilized treatment”

✓ located verbatim in the source · abstract, characters 365–548 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Sterilization significantly decreased the percentage of mycorrhizal infection in soybean.

Taxon
soybeanGlycine maxe0
Treatment
sterilizatione3
Interactor
mycorrhizale3symbiont
Outcome
percentage of mycorrhizal infectione3
Direction
decreasee3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“growth characteristics of soybean plant”

✓ located verbatim in the source · abstract, characters 323–362 · show in text ↑
e3

“The percentage of mycorrhizal infection was decrease significantly by sterilization”

✓ located verbatim in the source · abstract, characters 681–764 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Replicated formalin treatment gave the lowest mycorrhizal infection percentage, 20%, compared with control.

Taxon
soybeanGlycine maxe0
Treatment
replicated formalin treatmente4
Interactor
mycorrhizale4symbiont
Outcome
mycorrhizal infection percentagee4
Direction
loweste5
Value
20%e4
Comparator
controle4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“growth characteristics of soybean plant”

✓ located verbatim in the source · abstract, characters 323–362 · show in text ↑
e4

“the mycorrhizal infection percentage of replicated formalin treatment was 20% in compared with 71.60 , 51.66 ,35 and 31.66% for control”

✓ located verbatim in the source · abstract, characters 1177–1312 · show in text ↑
e5

“the replicated formaline treatment gave the lowest value”

✓ located verbatim in the source · abstract, characters 913–969 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Autoclave treatment gave higher growth characteristic values than formalin treatment.

Taxon
soybeanGlycine maxe0
Treatment
Autoclave treatmente6
Outcome
growth characteristics valuee6
Direction
highere6
Comparator
formaline treatmente6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“growth characteristics of soybean plant”

✓ located verbatim in the source · abstract, characters 323–362 · show in text ↑
e6

“the growth characteristics value of Autoclave treatment was higher than formaline treatment”

✓ located verbatim in the source · abstract, characters 1663–1754 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Replicated formalin treatment gave the lowest soybean germination percentage.

Taxon
soybeanGlycine maxe0
Treatment
formaline replicated treatmente7
Outcome
germination percentagee7
Direction
loweste7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“growth characteristics of soybean plant”

✓ located verbatim in the source · abstract, characters 323–362 · show in text ↑
e7

“the formaline replicated treatment gave the lowest value of germination percentage”

✓ located verbatim in the source · abstract, characters 1757–1839 · show in text ↑