Abstract
The worldwide increase in grapevine trunk diseases, mainly esca, represents a major threat for vineyard sustainability. Biocontrol of a pioneer fungus of esca, Phaeomoniella chlamydospora, was investigated here by deciphering the tripartite interaction between this trunk-esca pathogen, grapevine and the biocontrol-oomycete, Pythium oligandrum. When P. oligandrum colonizes grapevine roots, it was observed that the wood necroses caused by P. chlamydospora were significantly reduced. Transcriptomic analyses of plant and fungus responses were performed to determine the molecular events occurring, with the aim to relate P.chlamydospora degradation of wood to gene expression modulation. Following P. oligandrum-root colonization, major transcriptomic changes occurred both, in the grapevine-defense system and in the P. chlamydospore-virulence factors. Grapevine-defense was enhanced in response to P. chlamydospora attacks, with P. oligandrum acting as a plant-systemic resistance inducer, promoting jasmonic/ethylene signaling pathways and grapevine priming. P. chlamydospora pathogenicity genes, such as those related to secondary metabolite biosynthesis, carbohydrate-active enzymes and transcription regulators, were also affected in their expression. Shifts in grapevine responses and key-fungal functions were associated with the reduction of P. chlamydospora wood necroses. This study provides evidence of wood fungal pathogen transcriptional changes induced by a root biocontrol agent, P. oligandrum, in which there is no contact between the two microorganisms.
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Claims 7
Claim · model-written summary
Pythium oligandrum colonization of grapevine roots significantly reduced wood necroses caused by Phaeomoniella chlamydospora.
- Treatment
- P. oligandrum colonizes grapevine rootse1
- Interactor
- P. chlamydosporaPhaeomoniella chlamydosporae1pathogen
- Second interactor
- P. oligandrum
Pythium oligandrume1biocontrol_agent - Outcome
- wood necrosese1
- Direction
- reducede1
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“When P. oligandrum colonizes grapevine roots, it was observed that the wood necroses caused by P. chlamydospora were significantly reduced”
Claim · model-written summary
Following P. oligandrum root colonization, major transcriptomic changes occurred in the grapevine-defense system.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Following P. oligandrum-root colonization, major transcriptomic changes occurred both, in the grapevine-defense system and in the P. chlamydospore-virulence factors”
Claim · model-written summary
Following P. oligandrum root colonization, major transcriptomic changes occurred in the virulence factors of Phaeomoniella chlamydospora, which had no contact with P. oligandrum.
- Treatment
- P. oligandrum-root colonizatione1
- Interactor
- P. chlamydosporee1pathogen
- Second interactor
- P. oligandrumPythium oligandrume1biocontrol_agent
- Outcome
- virulence factorse1
- Qualifier
- there is no contact between the two microorganismse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Following P. oligandrum-root colonization, major transcriptomic changes occurred both, in the grapevine-defense system and in the P. chlamydospore-virulence factors”
e2“in which there is no contact between the two microorganisms”
Claim · model-written summary
Grapevine defence was enhanced in response to Phaeomoniella chlamydospora attacks.
- Interactor
- P. chlamydosporaPhaeomoniella chlamydosporae1pathogen
- Outcome
- Grapevine-defensee1
- Direction
- enhancede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Grapevine-defense was enhanced in response to P. chlamydospora attacks”
Claim · model-written summary
Pythium oligandrum acts as a plant-systemic resistance inducer in grapevine, promoting jasmonic/ethylene signalling pathways and grapevine priming.
- Interactor
- P. oligandrumPythium oligandrume1biocontrol_agent
- Outcome
- jasmonic/ethylene signaling pathwayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“P. oligandrum acting as a plant-systemic resistance inducer, promoting jasmonic/ethylene signaling pathways and grapevine priming”
Claim · model-written summary
Following P. oligandrum root colonization, pathogenicity genes of Phaeomoniella chlamydospora (secondary metabolite biosynthesis, carbohydrate-active enzymes, transcription regulators) were affected in expression.
- Treatment
- P. oligandrum-root colonizatione1
- Interactor
- P. chlamydosporaPhaeomoniella chlamydosporae2pathogen
- Outcome
- pathogenicity genese2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Following P. oligandrum-root colonization, major transcriptomic changes occurred both, in the grapevine-defense system and in the P. chlamydospore-virulence factors”
e2“P. chlamydospora pathogenicity genes, such as those related to secondary metabolite biosynthesis, carbohydrate-active enzymes and transcription regulators, were also affected in their expression”
Claim · model-written summary
Shifts in grapevine responses and key fungal functions were associated with reduced wood necroses caused by Phaeomoniella chlamydospora.
- Interactor
- P. chlamydosporaPhaeomoniella chlamydosporae1pathogen
- Outcome
- wood necrosese1
- Direction
- reductione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Shifts in grapevine responses and key-fungal functions were associated with the reduction of P. chlamydospora wood necroses”
The same questions in other papers
Pythium oligandrum · Phaeomoniella chlamydospora · VitisResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.