Abstract
BACKGROUND AND OBJECTIVE: Heat shock proteins are induced by high temperature and other environmental stimuli to protect cellular proteins. Despite extensive research on the molecular response to heat stress, the effect of high temperatures on genes and pathways remains unclear. This study investigated the expression of the HSP17 gene in nine Egyptian wheat varieties and the role of HSP17 promoter CpG methylation in the regulation of HSP17 under high temperature. MATERIALS AND METHODS: The HSP17 expression was investigated by using semi-quantitative PCR analysis. Methylation at the HSP17 promoter proximal region was analyzed using bisulphite sequencing and CpG viewer software. RESULTS: Under normal conditions, HSP17 and methyltransferase 3 (MET3) exhibited similar expression levels in the 9 studied varieties. After exposure to high temperature, the expression level of HSP17 in Giza155 was barely detected. Among the nine varieties, the expression level of HSP17 was highest in Giza168 (11.3 folds of Giza155). Analysis of methylation of 14 CpG islands at the HSP17 proximal promoter sequence showed that methylation of 10 CpG islands differed only by 10-20%, whereas methylation at the other 4 CpGs differed by 56.7-60%. The high expression of HSP17 in Giza168 in response to high temperature was associated with low methylation of four CpGs and low MET3 expression, whereas low expression of HSP17 in Giza155 was associated with high methylation and high MET3 expression. CONCLUSION: The results can aid the development of next-generation approaches to the evaluation of commercial wheat varieties and the development of next-generation approaches to plant breeding employing epiallele integration.
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Claims 9
Claim · model-written summary
HSP17 and MET3 had similar expression levels across nine wheat varieties in normal conditions.
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- HSP17 and methyltransferase 3 (MET3)e0
- Outcome
- similar expression levelse0
- Qualifier
- Under normal conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under normal conditions, HSP17 and methyltransferase 3 (MET3) exhibited similar expression levels in the 9 studied varieties.”
t0“Wheat Varieties”
Claim · model-written summary
HSP17 expression was barely detected in Giza155 after high temperature exposure.
- Taxon
- WheatTriticum aestivumt0
- Material
- Giza155e0
- Gene
- HSP17e0
- Outcome
- expression level of HSP17e0
- Direction
- barely detectede0
- Qualifier
- high temperaturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After exposure to high temperature, the expression level of HSP17 in Giza155 was barely detected.”
t0“Wheat Varieties”
Claim · model-written summary
HSP17 expression in Giza168 was highest among the nine varieties, at 11.3 folds of Giza155.
- Taxon
- WheatTriticum aestivumt0
- Material
- Giza168e0
- Gene
- HSP17e0
- Outcome
- expression level of HSP17e0
- Direction
- higheste0
- Value
- 11.3 foldse0
- Comparator
- Giza155e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the nine varieties, the expression level of HSP17 was highest in Giza168 (11.3 folds of Giza155).”
t0“Wheat Varieties”
Claim · model-written summary
Methylation of 10 HSP17 promoter CpG islands differed by 10-20% among varieties.
- Taxon
- WheatTriticum aestivumt0
- Gene
- HSP17e1
- Outcome
- methylation of 10 CpG islandse0
- Direction
- differede0
- Value
- 10-20%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“methylation of 10 CpG islands differed only by 10-20%”
e1“HSP17 proximal promoter sequence”
t0“Wheat Varieties”
Claim · model-written summary
Methylation of the other four HSP17 promoter CpGs differed by 56.7-60% among varieties.
- Taxon
- WheatTriticum aestivumt0
- Gene
- HSP17e1
- Outcome
- methylation at the other 4 CpGse0
- Direction
- differede0
- Value
- 56.7-60%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“methylation at the other 4 CpGs differed by 56.7-60%”
e1“HSP17 proximal promoter sequence”
t0“Wheat Varieties”
Claim · model-written summary
High heat-responsive HSP17 expression in Giza168 was associated with low methylation of four CpGs.
- Taxon
- WheatTriticum aestivumt0
- Material
- Giza168e0
- Gene
- HSP17e0
- Outcome
- high expression of HSP17e0
- Direction
- highe0
- Qualifier
- low methylation of four CpGse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high expression of HSP17 in Giza168 in response to high temperature was associated with low methylation of four CpGs”
t0“Wheat Varieties”
Claim · model-written summary
High heat-responsive HSP17 expression in Giza168 was associated with low MET3 expression.
- Taxon
- WheatTriticum aestivumt0
- Material
- Giza168e0
- Gene
- HSP17e0
- Outcome
- high expression of HSP17e0
- Direction
- highe0
- Qualifier
- low MET3 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high expression of HSP17 in Giza168 in response to high temperature was associated with low methylation of four CpGs and low MET3 expression”
t0“Wheat Varieties”
Claim · model-written summary
Low HSP17 expression in Giza155 was associated with high methylation.
- Taxon
- WheatTriticum aestivumt0
- Material
- Giza155e0
- Gene
- HSP17e0
- Outcome
- low expression of HSP17e0
- Direction
- lowe0
- Qualifier
- high methylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low expression of HSP17 in Giza155 was associated with high methylation and high MET3 expression”
t0“Wheat Varieties”
Claim · model-written summary
Low HSP17 expression in Giza155 was associated with high MET3 expression.
- Taxon
- WheatTriticum aestivumt0
- Material
- Giza155e0
- Gene
- HSP17e0
- Outcome
- low expression of HSP17e0
- Direction
- lowe0
- Qualifier
- high MET3 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low expression of HSP17 in Giza155 was associated with high methylation and high MET3 expression”
t0“Wheat Varieties”