Abstract
Banana is a key staple food and fruit in countries all over the world. However, the development of the global banana industry is seriously threatened by Fusarium wilt disease, which is caused by Fusarium oxysporum f. sp. cubense (Foc). In particular, Foc tropical race 4 (Foc TR4) could infect more than 80% of global banana and plantain crops. Until now, there were no commercial chemicals or resistant cultivars available to control the disease. Biological control using actinomycetes is considered a promising strategy. In this study, 88 actinomycetes were isolated from a banana orchard without symptoms of Fusarium wilt disease for more than 10 years. An actinobacterial strain labeled as JBS5-6 has exhibited strong antifungal activities against Foc TR4 and other selected 10 phytopathogenic fungi. Based on phenotypic and biochemical traits as well as complete genome analysis, strain JBS5-6 was assigned to Streptomyces violaceusniger. Extracts of the strain inhibited the mycelial growth and spore germination of Foc TR4 by destroying membrane integrity and the ultrastructure of cells. The complete genome of strain JBS5-6 was sequenced and revealed a number of key function gene clusters that contribute to the biosynthesis of active secondary metabolites. Sixteen chemical compounds were further identified by gas chromatography-mass spectrometry (GC-MS). 5-hydroxymethyl-2-furancarboxaldehyde was one of the dominant components in strain JBS5-6 extracts. Moreover, fermentation broth of strain JBS5-6 significantly reduced the disease index of banana seedlings by inhibiting the infection of Foc TR4 in a pot experiment. Hence, strain JBS5-6 is a potential biocontrol agent for the management of disease and the exploitation of biofertilizer.
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Claims 7
Claim · model-written summary
Strain JBS5-6 showed strong antifungal activity against Foc TR4 and other phytopathogenic fungi.
- Taxon
- Bananat0
- Interactor
- Foc TR4
_(38100977215).jpg?width=60)
Fusarium odoratissimume0pathogen - Second interactor
- JBS5-6e0biocontrol_agent
- Outcome
- antifungal activitiese0
- Qualifier
- stronge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(38100977215).jpg?width=500)
Verbatim
t0“Control Banana Fusarium Wilt”
e0“An actinobacterial strain labeled as JBS5-6 has exhibited strong antifungal activities against Foc TR4 and other selected 10 phytopathogenic fungi”
Claim · model-written summary
Extracts of strain JBS5-6 inhibited mycelial growth and spore germination of Foc TR4 by destroying membrane integrity and cell ultrastructure.
- Taxon
- Bananat0
- Treatment
- Extracts of the straine1
- Interactor
- Foc TR4Fusarium odoratissimume1pathogen
- Outcome
- mycelial growth and spore germinatione1
- Direction
- inhibitede1
- Qualifier
- by destroying membrane integrity and the ultrastructure of cellse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Control Banana Fusarium Wilt”
e1“Extracts of the strain inhibited the mycelial growth and spore germination of Foc TR4 by destroying membrane integrity and the ultrastructure of cells”
Claim · model-written summary
The complete genome of strain JBS5-6 contains key function gene clusters for active secondary metabolite biosynthesis.
Verbatim
t0“Control Banana Fusarium Wilt”
e2“The complete genome of strain JBS5-6 was sequenced and revealed a number of key function gene clusters”
Claim · model-written summary
Sixteen chemical compounds were identified in the strain extracts by GC-MS.
Verbatim
t0“Control Banana Fusarium Wilt”
e3“Sixteen chemical compounds were further identified by gas chromatography-mass spectrometry (GC-MS)”
Claim · model-written summary
5-hydroxymethyl-2-furancarboxaldehyde was one of the dominant components of strain JBS5-6 extracts.
Verbatim
t0“Control Banana Fusarium Wilt”
e4“5-hydroxymethyl-2-furancarboxaldehyde was one of the dominant components in strain JBS5-6 extracts”
Claim · model-written summary
Fermentation broth of strain JBS5-6 significantly reduced the disease index of banana seedlings in a pot experiment.
- Taxon
- bananae5
- Treatment
- fermentation broth of strain JBS5-6e5
- Interactor
- Foc TR4Fusarium odoratissimume5pathogen
- Second interactor
- strain JBS5-6e5biocontrol_agent
- Outcome
- disease indexe5
- Direction
- reducede5
- Qualifier
- by inhibiting the infectione5
- Setting
- pot experimente5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Control Banana Fusarium Wilt”
e5“fermentation broth of strain JBS5-6 significantly reduced the disease index of banana seedlings by inhibiting the infection of Foc TR4 in a pot experiment”
Claim · model-written summary
Strain JBS5-6 was assigned to Streptomyces violaceusniger on phenotypic, biochemical and genomic traits.
Verbatim
t0“Control Banana Fusarium Wilt”
e6“strain JBS5-6 was assigned to Streptomyces violaceusniger”
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