Abstract
Evaluation of genetic diversity is the key principal for plant breeding, providing an opportunity to discover novel characters and alleles for breeders. In the present study, 69 durum wheat genotypes were investigated for genetic diversity using several CAAT box-derived polymorphism (CBDP) and inter-simple sequence repeat (ISSR) markers. Twelve CBDP and sixteen ISSR primers amplified a total of 115 and 160 polymorphic fragments with a mean of 9.58 and 10 fragments per primer, respectively. CBDP primers showed the higher mean values for informativeness parameters such as polymorphic information content (PIC), resolving power (Rp) and marker index (MI) in comparison with ISSR primers. The results of analysis of molecular variance (AMOVA) indicated that the highest proportion of genetic variance referred within populations. Furthermore, CBDP primers indicated high values for all genetic parameters. Besides, the highest values of genetic parameters including number of observed (Na) and effective alleles (Ne), Shannon?s information index (I) and Nei?s gene diversity (He) were estimated for Iranian durum wheat landraces. Cluster analysis based on each molecular technique classified all durum wheat genotypes into three main groups, so that the results of principal coordinate analysis (PCoA) supported the grouping patterns. As a result, the grouping pattern observed by ISSR primers was clearer than CBDP primers and grouped all samples based on their origins. However, Mantel?s coefficient correlation test illustrated the higher positive correlation (0.54) between both marker techniques. Hence, the use of these markers in combination with each other to evaluate the genetic diversity is recommended.
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Claims 10
Claim · model-written summary
CBDP primers amplified 115 polymorphic fragments, averaging 9.58 per primer.
- Taxon
- durum wheat
Triticum turgidum subsp. durumt0 - Outcome
- polymorphic fragmentse0
- Value
- 115e0
- Qualifier
- 9.58e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“Twelve CBDP and sixteen ISSR primers amplified a total of 115 and 160 polymorphic fragments with a mean of 9.58 and 10 fragments per primer, respectively.”
Claim · model-written summary
ISSR primers amplified 160 polymorphic fragments, averaging 10 per primer.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- polymorphic fragmentse0
- Value
- 160e0
- Qualifier
- 10 fragments per primere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“Twelve CBDP and sixteen ISSR primers amplified a total of 115 and 160 polymorphic fragments with a mean of 9.58 and 10 fragments per primer, respectively.”
Claim · model-written summary
CBDP primers had higher mean informativeness parameters than ISSR primers.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- informativeness parameterse0
- Direction
- highere0
- Comparator
- ISSR primerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“CBDP primers showed the higher mean values for informativeness parameters such as polymorphic information content (PIC), resolving power (Rp) and marker index (MI) in comparison with ISSR primers.”
Claim · model-written summary
The greatest share of genetic variance occurred within populations.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- highest proportion of genetic variance referred within populationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“The results of analysis of molecular variance (AMOVA) indicated that the highest proportion of genetic variance referred within populations.”
Claim · model-written summary
CBDP primers yielded high values across genetic parameters.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- high values for all genetic parameterse0
- Value
- high valuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“Furthermore, CBDP primers indicated high values for all genetic parameters.”
Claim · model-written summary
Iranian durum wheat landraces had the highest observed and effective allele counts, Shannon index and Nei diversity.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Material
- Iranian durum wheat landracese0
- Outcome
- highest values of genetic parameterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“the highest values of genetic parameters including number of observed (Na) and effective alleles (Ne), Shannon?s information index (I) and Nei?s gene diversity (He) were estimated for Iranian durum wheat landraces.”
Claim · model-written summary
Both marker techniques classified durum wheat genotypes into three groups, supported by principal coordinate analysis.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- classified all durum wheat genotypes into three main groupse0
- Value
- three main groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“Cluster analysis based on each molecular technique classified all durum wheat genotypes into three main groups, so that the results of principal coordinate analysis (PCoA) supported the grouping patterns.”
Claim · model-written summary
ISSR primers grouped samples by origin more clearly than CBDP primers.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- grouping patterne0
- Direction
- clearere0
- Comparator
- CBDP primerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“the grouping pattern observed by ISSR primers was clearer than CBDP primers and grouped all samples based on their origins.”
Claim · model-written summary
The marker techniques had a positive Mantel correlation of 0.54.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- positive correlatione0
- Value
- 0.54e0
- Comparator
- both marker techniquese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“Mantel?s coefficient correlation test illustrated the higher positive correlation (0.54) between both marker techniques.”
Claim · model-written summary
The authors recommend using both marker types together to assess genetic diversity.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- use of these markers in combination with each other to evaluate the genetic diversitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“durum wheat”
e0“the use of these markers in combination with each other to evaluate the genetic diversity is recommended.”