Abstract
Bacterial panicle blight (BPB), caused by Burkholderia glumae, is one of the most severe seed-borne bacterial diseases of rice in the world, which can decrease rice production by ≤75%. Nevertheless, there are few effective measures to manage this disease. In an attempt to develop an alternative management tool for BPB, we isolated and characterized phages from soil and water that are effective to lyse several strains of B. glumae. After tests of host ranges, the phages NBP1-1, NBP4-7, and NBP4-8 were selected for further comprehensive characterization, all of which could lyse B. glumae BGLa14-8 (phage sensitive) but not B. glumae 336gr-1 (phage insensitive). This result indicates that the phages killing B. glumae cells have specific host ranges at the strain level within the bacterial species. In the greenhouse condition of this study, foliar application of the phage NBP4-7 reduced the severity of BPB caused by B. glumae BGLa14-8 ≤62% but did not cause any significant effect on the infection by B. glumae 336gr-1. Electron microscopy and whole-genome sequencing were also performed to characterize the three selected phages. Transmission electron microscopy revealed that the selected phages belong to the family Myoviridae. Furthermore, whole-genome sequence analysis indicated that the three phages belong to a same species and are closely related to the Burkholderia phage KL3, a member of the Myoviridae family.
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Claims 5
Claim · model-written summary
Phages NBP1-1, NBP4-7 and NBP4-8 lysed B. glumae BGLa14-8 but not B. glumae 336gr-1
- Taxon
- rice
Oryza sativae4 - Interactor
- B. glumae
Burkholderia glumaee0pathogen - Second interactor
- phages NBP1-1, NBP4-7, and NBP4-8e0biocontrol_agent
- Outcome
- host rangese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“seed-borne bacterial diseases of rice”
e0“After tests of host ranges, the phages NBP1-1, NBP4-7, and NBP4-8 were selected for further comprehensive characterization, all of which could lyse B. glumae BGLa14-8 (phage sensitive) but not B. glumae 336gr-1 (phage insensitive)”
Claim · model-written summary
Foliar application of phage NBP4-7 reduced BPB severity caused by B. glumae BGLa14-8 by up to 62% in the greenhouse
- Taxon
- riceOryza sativae4
- Treatment
- foliar application of the phage NBP4-7e1
- Interactor
- B. glumae BGLa14-8Burkholderia glumaee1pathogen
- Second interactor
- NBP4-7e1biocontrol_agent
- Outcome
- severity of BPBe1
- Direction
- reducede1
- Value
- ≤62%e1
- Setting
- greenhouse conditione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“seed-borne bacterial diseases of rice”
e1“In the greenhouse condition of this study, foliar application of the phage NBP4-7 reduced the severity of BPB caused by B. glumae BGLa14-8 ≤62% but did not cause any significant effect on the infection by B. glumae 336gr-1”
Claim · model-written summary
Foliar application of phage NBP4-7 did not significantly affect infection by B. glumae 336gr-1 in the greenhouse
- Taxon
- riceOryza sativae4
- Treatment
- foliar application of the phage NBP4-7e1
- Interactor
- B. glumae 336gr-1Burkholderia glumaee1pathogen
- Second interactor
- NBP4-7e1biocontrol_agent
- Outcome
- infection by B. glumae 336gr-1e1
- Direction
- no_changeinferrede1
- Setting
- greenhouse conditione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“seed-borne bacterial diseases of rice”
e1“In the greenhouse condition of this study, foliar application of the phage NBP4-7 reduced the severity of BPB caused by B. glumae BGLa14-8 ≤62% but did not cause any significant effect on the infection by B. glumae 336gr-1”
Claim · model-written summary
The selected phages belong to the family Myoviridae
- Taxon
- riceOryza sativae4
- Outcome
- the selected phages belong to the family Myoviridaee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“seed-borne bacterial diseases of rice”
e2“Transmission electron microscopy revealed that the selected phages belong to the family Myoviridae”
Claim · model-written summary
The three phages belong to a same species and are closely related to Burkholderia phage KL3
- Taxon
- riceOryza sativae4
- Outcome
- the three phages belong to a same speciese3
- Qualifier
- closely related to the Burkholderia phage KL3e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“seed-borne bacterial diseases of rice”
e3“the three phages belong to a same species and are closely related to the Burkholderia phage KL3”
The same questions in other papers
application · Burkholderia glumae · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.