Paper · 2020 · The Bulletin of Irkutsk State University Series Biology Ecology

Differently Directional Effects of Tebuconazole-Based Disinfectant of Seeds “Bunker” on the Growth of Winter Wheat Shoots and Roots

O. I. Grabelnych; E. A. Polykova; A. V. Korsukova; et al.

6
claims
6
verbatim quotes
3
citations
2
research areas
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Open access · cc-by-nc

Abstract

Derivatives of a triazole are used not only as fungicides to prevent infection of plants, but also for treatment. These drugs are actively used against plant diseases caused by basidiomycetes, ascomycetes, and some deuteromycetes. Due to their chemical stability, they have a long-term protective effect, and their solubility in water ensures their movement from the roots to the aerial part of plants. The aim of this work was to study the effect of seed treatment with tebuconazole-containing preparation “Bunker” on the growth of shoots and roots of winter wheat in the light and physiological and biochemical parameters. We used a fungicide of prophylactic and therapeutic action tebuconazole-containing seed treater “Bunker” (August, Russia), the active ingredient of which is tebuconazole (60 g/L). The shoot length and total root length, wet and dry biomass, as well as the content of photosynthetic pigments, reactive oxygen species – hydrogen peroxide (H2O2) and lipid peroxidation products (LPO) reacting with thiobarbituric acid (TBA-RP) in the tissues of shoots and roots were analyzed. The content of chlorophylls a and b and carotenoids in the extract was determined spectrophotometrically at wavelengths of 665, 649, and 440 nm, respectively. The obtained results indicate that the action of the drug is aimed at inhibiting the growth of shoots and reducing the ratio of shoot length to root length, while on the 7-9th day of growth, the effectiveness of its action is higher, and the most significant effect is exerted by an increased dose of the disinfectant (1.5 μL/g). It was found statistically significant stimulation of root growth on the 9th day of cultivation, regardless of the dose of the dressing agent. Despite the significant growth-inhibiting effect, treatment with Bunker was not accompanied by activation of oxidative processes in shoot tissues, with the exception of an increase in the content of H2O2 and TBA-RP at the initial stage of growth (5 days). In root tissues, the content of H2O2 and TBA did not change under the action of the studied preparation. No differences in the content of chlorophylls a and b and carotenoids in the tissues of wheat shoots after treatment with the studied fungicide were found. Based on the data obtained, it can be concluded that seed dressing agents containing tebuconazole as an active ingredient can be used to ensure plant resistance to moisture deficiency and improve mineral nutrition.

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Claims 6

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Bunker inhibited wheat shoot growth and reduced the shoot-to-root length ratio, especially on days 7–9 and at the higher dose.

Treatment
druge0
Outcome
growth of shoots and reducing the ratio of shoot length to root lengthe0
Direction
inhibitinge0
Qualifier
7-9th day of growthe0

Not reportedTaxonComparator

Verbatim

e0

“the action of the drug is aimed at inhibiting the growth of shoots and reducing the ratio of shoot length to root length, while on the 7-9th day of growth, the effectiveness of its action is higher, and the most significant effect is exerted by an increased dose of the disinfectant (1.5 μL/g)”

✓ located verbatim in the source · abstract, characters 1293–1586 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The seed dressing agent significantly stimulated root growth on day 9 regardless of dose.

Treatment
dressing agente0
Outcome
root growthe0
Direction
stimulatione0
Qualifier
9th day of cultivatione0

Not reportedTaxonComparator

Verbatim

e0

“statistically significant stimulation of root growth on the 9th day of cultivation, regardless of the dose of the dressing agent”

✓ located verbatim in the source · abstract, characters 1601–1729 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bunker treatment increased shoot hydrogen peroxide and TBA-reactive products at the initial growth stage.

Organ / stage
shoot tissuese0
Treatment
treatment with Bunkere0
Outcome
content of H2O2 and TBA-RPe0
Direction
increasee0
Qualifier
initial stage of growth (5 days)e0

Not reportedTaxonComparator

Verbatim

e0

“treatment with Bunker was not accompanied by activation of oxidative processes in shoot tissues, with the exception of an increase in the content of H2O2 and TBA-RP at the initial stage of growth (5 days)”

✓ located verbatim in the source · abstract, characters 1781–1985 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Bunker treatment did not change root H2O2 or TBA content.

Organ / stage
root tissuese0
Treatment
studied preparatione0
Outcome
content of H2O2 and TBAe0
Direction
did not changee0

Not reportedTaxonComparator

Verbatim

e0

“In root tissues, the content of H2O2 and TBA did not change under the action of the studied preparation”

✓ located verbatim in the source · abstract, characters 1987–2090 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Wheat shoot chlorophyll a, chlorophyll b, and carotenoid content did not differ after fungicide treatment.

Organ / stage
shootse0
Treatment
treatment with the studied fungicidee0
Outcome
content of chlorophylls a and b and carotenoidse0
Direction
No differencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“No differences in the content of chlorophylls a and b and carotenoids in the tissues of wheat shoots after treatment with the studied fungicide were found”

✓ located verbatim in the source · abstract, characters 2092–2246 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors conclude that tebuconazole seed dressings can be used to support resistance to moisture deficit and mineral nutrition.

Treatment
seed dressing agents containing tebuconazolee0
Outcome
plant resistance to moisture deficiency and improve mineral nutritione0

Not reportedTaxon

Verbatim

e0

“seed dressing agents containing tebuconazole as an active ingredient can be used to ensure plant resistance to moisture deficiency and improve mineral nutrition”

✓ located verbatim in the source · abstract, characters 2301–2461 · show in text ↑

fungicide · physiology: pigments · Triticum aestivum (wheat)Positive and negative results

5 papers: 2 positive, 3 negative or no effect

2 papers in the same direction as this paper · 2 in the opposite direction

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  2. positive
  3. negative

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.