Abstract
The leaf is the main photosynthetic organ in plants, such as bamboo. Leaves from bamboo are used as a food additive. However, according to our investigation, to date there have been no reports concerning the leaf development of bamboo. By measuring over 7500 parenchymal cells, we discovered that the linear leaf growth of Pseudosasa japonica could be divided into three growth sections. The first one is a roughly 1-cm long division zone (DZ), containing about 1580 cells, located at the bottom of the leaf; the second one is an about 3-cm long elongation zone (EZ), with ~1905 cells, located above the DZ; and the last is a mature zone (MZ) in which cell elongation is completed. The cell production rate of the DZ was 25.33-35.81 cells per hour, with an average of 29.73; and the cell division rate was ~0.45 cells per cell every 24 h. PacBio and Illumina transcriptome sequencing found 21 933 unigenes expressed in these zones. Further analysis revealed a dynamic transcriptome, with transcripts for cell division in the DZ changing to transcripts for cell elongation, photosynthetic development, secondary metabolism, stress resistance, and nutrition transport toward the leaf distal. Those transcriptome transformations correlated well with the changes of relative water content, biomass accumulation, and cellulose crystal degree and were supported by quantitative polymerase chain reaction data. These results revealed a developmental gradient of the bamboo linear growth leaf, which offers a foundation to elucidate and engineer leaf development in bamboo, an economically valuable plant.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Linear leaf growth had three zones.
- Taxon
- Pseudosasa japonica
Pseudosasa japonicae0 - Outcome
- three growth sectionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the linear leaf growth of Pseudosasa japonica could be divided into three growth sections.”
Claim · model-written summary
The basal division zone was about 1 cm and contained about 1580 cells.
- Organ / stage
- division zone (DZ)e0
- Outcome
- roughly 1-cm longe0
- Value
- roughly 1-cme0
- Qualifier
- about 1580 cellse0
Not reportedTaxon
Verbatim
e0“a roughly 1-cm long division zone (DZ), containing about 1580 cells, located at the bottom of the leaf”
Claim · model-written summary
The elongation zone was about 3 cm and contained about 1905 cells.
Not reportedTaxon
Verbatim
e0“an about 3-cm long elongation zone (EZ), with ~1905 cells, located above the DZ”
Claim · model-written summary
Cell elongation was complete in the mature zone.
Not reportedTaxon
Verbatim
e0“a mature zone (MZ) in which cell elongation is completed.”
Claim · model-written summary
Division zone cell production was 25.33 to 35.81 cells per hour.
Not reportedTaxon
Verbatim
e0“The cell production rate of the DZ was 25.33-35.81 cells per hour, with an average of 29.73”
Claim · model-written summary
The cell division rate was about 0.45 cells per cell per 24 hours.
Not reportedTaxon
Verbatim
e0“the cell division rate was ~0.45 cells per cell every 24 h.”
Claim · model-written summary
Transcriptome sequencing found 21 933 unigenes in leaf growth zones.
- Treatment
- PacBio and Illumina transcriptome sequencinge0
- Outcome
- unigenes expressed in these zonese0
- Value
- 21 933e0
Not reportedTaxon
Verbatim
e0“PacBio and Illumina transcriptome sequencing found 21 933 unigenes expressed in these zones.”
Claim · model-written summary
Transcript functions shifted along the leaf from division to elongation and other distal processes.
- Outcome
- transcripts for cell division in the DZ changing to transcripts for cell elongation, photosynthetic development, secondary metabolism, stress resistance, and nutrition transporte0
- Direction
- changinge0
Not reportedTaxon
Verbatim
e0“transcripts for cell division in the DZ changing to transcripts for cell elongation, photosynthetic development, secondary metabolism, stress resistance, and nutrition transport toward the leaf distal.”
Claim · model-written summary
Transcriptome changes correlated with water content, biomass, and cellulose crystallinity changes.
- Organ / stage
- leafe0
- Outcome
- changes of relative water content, biomass accumulation, and cellulose crystal degreee0
- Qualifier
- transcriptome transformationse0
Not reportedTaxonDirection
Verbatim
e0“leaf distal. Those transcriptome transformations correlated well with the changes of relative water content, biomass accumulation, and cellulose crystal degree”