Abstract
Leaf sheath explants of sugarcane variety CoS 99259 were inoculated on agar gelled (8.0 g/l) Murashige and Skoog’s (MS) medium containing 3.0 mg/l of 2, 4 dichlorophenoxyacetic acid (2, 4-D). Different sources of carbon viz., sucrose, glucose, mill sugar, sugar cubes, and fructose were separately added into the medium each at a concentration of 20 g/l. The result showed that maximum 64.4% of leaf sheath explants initiated callus within 3 weeks of inoculation on medium containing sucrose followed by sugar cubes (59.9%). The best growth of callus was also observed in presence of sucrose followed by sugar cubes. The frequency of callus formation in presence of mill sugar, glucose, and fructose was significantly low. The actively growing calli were transferred on MS shoot proliferation medium supplemented with BAP and Kinetin (0.5mg/leach) along with respective sources of carbon. The result revealed the highest induction of shoot primordia in 64.4% callus culture in presence of sucrose followed by 59.6% in the presence of sugar cubes, within 4-6 weeks of transfer. The highest number of shoot per culture (21.3) was recorded on the medium containing sucrose followed by sugar cubes (16.8) which was statistically similar. The explants showed poor responses regarding callus formation, shoot regeneration /and shoot growth on the medium containing mill sugar, glucose or fructose as carbon source.
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Claims 13
Claim · model-written summary
Sucrose gave the maximum callus induction frequency of 64.4% within three weeks.
Verbatim
e0“maximum 64.4% of leaf sheath explants initiated callus within 3 weeks of inoculation on medium containing sucrose followed by sugar cubes (59.9%)”
t0“sugarcane”
Claim · model-written summary
Sugar cubes gave a callus induction frequency of 59.9%, second to sucrose.
- Taxon
- sugarcanet0
- Organ / stage
- leaf sheath explantse0
- Treatment
- sugar cubese0
- Outcome
- leaf sheath explants initiated calluse0
- Value
- 59.9%e0
- Comparator
- sucrosee0
Not reportedDirection
Verbatim
e0“maximum 64.4% of leaf sheath explants initiated callus within 3 weeks of inoculation on medium containing sucrose followed by sugar cubes (59.9%)”
t0“sugarcane”
Claim · model-written summary
Sucrose produced the best callus growth.
Verbatim
e0“The best growth of callus was also observed in presence of sucrose followed by sugar cubes.”
t0“sugarcane”
Claim · model-written summary
Callus formation frequency with mill sugar was significantly low.
Not reportedComparator
Verbatim
e0“The frequency of callus formation in presence of mill sugar, glucose, and fructose was significantly low.”
t0“sugarcane”
Claim · model-written summary
Callus formation frequency with glucose was significantly low.
Not reportedComparator
Verbatim
e0“The frequency of callus formation in presence of mill sugar, glucose, and fructose was significantly low.”
t0“sugarcane”
Claim · model-written summary
Callus formation frequency with fructose was significantly low.
Not reportedComparator
Verbatim
e0“The frequency of callus formation in presence of mill sugar, glucose, and fructose was significantly low.”
t0“sugarcane”
Claim · model-written summary
Sucrose induced shoot primordia in 64.4% of callus cultures within four to six weeks.
Verbatim
e0“highest induction of shoot primordia in 64.4% callus culture in presence of sucrose followed by 59.6% in the presence of sugar cubes, within 4-6 weeks of transfer”
t0“sugarcane”
Claim · model-written summary
Sugar cubes induced shoot primordia in 59.6% of callus cultures.
- Taxon
- sugarcanet0
- Treatment
- sugar cubese0
- Outcome
- induction of shoot primordiae0
- Value
- 59.6%e0
- Qualifier
- within 4-6 weeks of transfere0
- Comparator
- sucrosee0
Not reportedDirection
Verbatim
e0“highest induction of shoot primordia in 64.4% callus culture in presence of sucrose followed by 59.6% in the presence of sugar cubes, within 4-6 weeks of transfer”
t0“sugarcane”
Claim · model-written summary
Sucrose produced the highest shoot number, 21.3 per culture.
Verbatim
e0“The highest number of shoot per culture (21.3) was recorded on the medium containing sucrose followed by sugar cubes (16.8) which was statistically similar.”
t0“sugarcane”
Claim · model-written summary
Sugar cubes produced 16.8 shoots per culture, statistically similar to sucrose.
Verbatim
e0“The highest number of shoot per culture (21.3) was recorded on the medium containing sucrose followed by sugar cubes (16.8) which was statistically similar.”
t0“sugarcane”
Claim · model-written summary
Mill sugar gave poor callus formation, shoot regeneration and shoot growth.
- Taxon
- sugarcanet0
- Treatment
- mill sugare0
- Outcome
- callus formation, shoot regeneration /and shoot growthe0
- Direction
- poore0
Not reportedComparator
Verbatim
e0“The explants showed poor responses regarding callus formation, shoot regeneration /and shoot growth on the medium containing mill sugar, glucose or fructose as carbon source.”
t0“sugarcane”
Claim · model-written summary
Glucose gave poor callus formation, shoot regeneration and shoot growth.
- Taxon
- sugarcanet0
- Treatment
- glucosee0
- Outcome
- callus formation, shoot regeneration /and shoot growthe0
- Direction
- poore0
Not reportedComparator
Verbatim
e0“The explants showed poor responses regarding callus formation, shoot regeneration /and shoot growth on the medium containing mill sugar, glucose or fructose as carbon source.”
t0“sugarcane”
Claim · model-written summary
Fructose gave poor callus formation, shoot regeneration and shoot growth.
- Taxon
- sugarcanet0
- Treatment
- fructosee0
- Outcome
- callus formation, shoot regeneration /and shoot growthe0
- Direction
- poore0
Not reportedComparator
Verbatim
e0“The explants showed poor responses regarding callus formation, shoot regeneration /and shoot growth on the medium containing mill sugar, glucose or fructose as carbon source.”
t0“sugarcane”
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