Abstract
Long-term agricultural practices are assumed to shape the rhizosphere microbiome of crops with implications for plant health. In a long-term field experiment, we investigated the effect of different tillage and fertilization practices on soil and barley rhizosphere microbial communities by means of amplicon sequencing of 16S rRNA gene fragments from total community DNA. Differences in the microbial community composition depending on the tillage practice, but not the fertilization intensity were revealed. To examine whether these soil and rhizosphere microbiome differences influence the plant defense response, barley (cultivar Golden Promise) was grown in field or standard potting soil under greenhouse conditions and challenged with Blumeria graminis f. sp. hordei (Bgh). Amplicon sequence analysis showed that preceding tillage practice, but also aboveground Bgh challenge significantly influenced the microbial community composition. Expression of plant defense-related genes PR1b and PR17b was higher in challenged compared to unchallenged plants. The Bgh infection rates were strikingly lower for barley grown in field soil compared to potting soil. Although previous agricultural management shaped the rhizosphere microbiome, no differences in plant health were observed. We propose therefore that the management-independent higher microbial diversity of field soils compared to potting soils contributed to the low infection rates of barley.
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Claims 6
Claim · model-written summary
Barley rhizosphere microbial community composition differed with tillage practice but not with fertilization intensity.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- tillage practicee1
- Outcome
- microbial community compositione1
- Direction
- Differencese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“soil and barley rhizosphere microbial communities”
e1“Differences in the microbial community composition depending on the tillage practice, but not the fertilization intensity were revealed”
Claim · model-written summary
Barley defense-related gene expression (PR1b and PR17b) was higher in plants challenged with Blumeria graminis f. sp. hordei than in unchallenged plants.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- challenged with Blumeria graminis f. sp. hordeie7
- Interactor
- Blumeria graminis f. sp. hordeiBlumeria hordeie7pathogen
- Gene
- PR1b and PR17be2
- Outcome
- Expression of plant defense-related genese2
- Direction
- highere2
- Comparator
- unchallenged plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soil and barley rhizosphere microbial communities”
e2“Expression of plant defense-related genes PR1b and PR17b was higher in challenged compared to unchallenged plants”
e7“barley (cultivar Golden Promise) was grown in field or standard potting soil under greenhouse conditions and challenged with Blumeria graminis f. sp. hordei (Bgh)”
Claim · model-written summary
Barley grown in field soil had strikingly lower Bgh infection rates than barley grown in potting soil.
- Taxon
- barleyHordeum vulgaree3
- Material
- Golden Promisee7
- Treatment
- field soile3
- Interactor
- Bghe3pathogen
- Outcome
- Bgh infection ratese3
- Direction
- lowere3
- Comparator
- potting soile3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soil and barley rhizosphere microbial communities”
e3“The Bgh infection rates were strikingly lower for barley grown in field soil compared to potting soil”
e7“barley (cultivar Golden Promise) was grown in field or standard potting soil under greenhouse conditions and challenged with Blumeria graminis f. sp. hordei (Bgh)”
Claim · model-written summary
Aboveground Bgh challenge significantly influenced barley rhizosphere microbial community composition.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- aboveground Bgh challengee4
- Outcome
- microbial community compositione4
- Direction
- significantly influencede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soil and barley rhizosphere microbial communities”
e4“Amplicon sequence analysis showed that preceding tillage practice, but also aboveground Bgh challenge significantly influenced the microbial community composition”
Claim · model-written summary
Previous agricultural management did not change barley plant health despite shaping the rhizosphere microbiome.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- previous agricultural managemente5
- Outcome
- plant healthe5
- Direction
- no differencese5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soil and barley rhizosphere microbial communities”
e5“Although previous agricultural management shaped the rhizosphere microbiome, no differences in plant health were observed”
Claim · model-written summary
Preceding tillage practice significantly influenced barley rhizosphere microbial community composition.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- preceding tillage practicee4
- Outcome
- microbial community compositione4
- Direction
- significantly influencede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soil and barley rhizosphere microbial communities”
e4“Amplicon sequence analysis showed that preceding tillage practice, but also aboveground Bgh challenge significantly influenced the microbial community composition”
The same questions in other papers
Blumeria hordei · molecular: expression · Hordeum vulgare (barley)Results agree
2 papers in the same direction as this paper
- positive
- positive
infection · molecular: expression · Hordeum vulgare (barley)Positive and negative results
4 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- negative
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.