Abstract
High seed cost due to poor seed yield severely limits the adoption of hybrid rice by farmers. Increasing the out-crossing rate is one of the key strategies to increase hybrid seed production. Out-crossing rate is highly influenced by the size of female floral traits, which capture pollen grains from male donor plants. In the current study, we identified 14 QTLs derived from the perennial wild rice Oryza longistaminata by composite interval mapping for five key floral traits: stigma length (five), style length (three), stigma breadth (two), stigma area (one), and pistil length (three). QTL analysis and correlation studies revealed that these stigma traits were positively correlated and pleiotropic to the stigma length trait. We selected the major-effect QTL qSTGL8.0 conferring long stigma phenotype for further fine mapping and marker-assisted selection. The qSTGL8.0 (~ 3.9 Mb) was fine mapped using newly developed internal markers and was narrowed down to ~ 2.9 Mb size (RM7356-RM256 markers). Further, the flanking markers were validated in a segregating population and in progenies from different genetic backgrounds. The markers PA08-03 and PA08-18 showed the highest co-segregation with the stigma traits. The qSTGL8.0 was introgressed into two cytoplasmic male sterile (CMS) lines, IR58025A and IR68897A, by foreground, background, and trait selection approaches. The qSTGL8.0 introgression lines in CMS backgrounds showed a significantly higher seed setting rate (2.5-3.0-fold) than the original CMS lines in test crosses with their corresponding maintainer lines. The newly identified QTLs especially qSTGL8.0, will be quite useful for increasing out-crossing rate and this will contribute to increase seed production and decrease seed cost.
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Claims 7
Claim · model-written summary
Fourteen QTLs from Oryza longistaminata were identified for five rice floral traits.
- Taxon
- Rice, O. sativa L.
Oryza sativat0 - Material
- Oryza longistaminatae0
- Outcome
- 14 QTLse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice, O. sativa L.”
e0“identified 14 QTLs derived from the perennial wild rice Oryza longistaminata by composite interval mapping for five key floral traits: stigma length (five), style length (three), stigma breadth (two), stigma area (one), and pistil length (three)”
Claim · model-written summary
The stigma traits were positively correlated and pleiotropic to stigma length.
- Taxon
- Rice, O. sativa L.Oryza sativat0
- Organ / stage
- stigmae0
- Outcome
- stigma traitse0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice, O. sativa L.”
e0“these stigma traits were positively correlated and pleiotropic to the stigma length trait”
Claim · model-written summary
The qSTGL8.0 interval was narrowed from about 3.9 Mb to about 2.9 Mb.
- Taxon
- Rice, O. sativa L.Oryza sativat0
- Gene
- qSTGL8.0e0
- Outcome
- qSTGL8.0e0
- Direction
- narrowed downe0
- Value
- ~ 2.9 Mbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice, O. sativa L.”
e0“The qSTGL8.0 (~ 3.9 Mb) was fine mapped using newly developed internal markers and was narrowed down to ~ 2.9 Mb size (RM7356-RM256 markers)”
Claim · model-written summary
Flanking markers were validated in segregating progeny and different genetic backgrounds.
- Taxon
- Rice, O. sativa L.Oryza sativat0
- Outcome
- flanking markerse0
- Comparator
- different genetic backgroundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice, O. sativa L.”
e0“the flanking markers were validated in a segregating population and in progenies from different genetic backgrounds”
Claim · model-written summary
PA08-03 and PA08-18 had the highest co-segregation with stigma traits.
- Taxon
- Rice, O. sativa L.Oryza sativat0
- Gene
- PA08-03 and PA08-18e0
- Outcome
- co-segregatione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice, O. sativa L.”
e0“The markers PA08-03 and PA08-18 showed the highest co-segregation with the stigma traits”
Claim · model-written summary
qSTGL8.0 introgression increased seed setting in the two CMS backgrounds by 2.5-3.0-fold against original lines.
- Taxon
- Rice, O. sativa L.Oryza sativat0
- Treatment
- qSTGL8.0 introgressione0
- Gene
- qSTGL8.0e0
- Outcome
- seed setting ratee0
- Direction
- highere0
- Value
- 2.5-3.0-folde0
- Comparator
- original CMS linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice, O. sativa L.”
e0“The qSTGL8.0 introgression lines in CMS backgrounds showed a significantly higher seed setting rate (2.5-3.0-fold) than the original CMS lines in test crosses with their corresponding maintainer lines”
Claim · model-written summary
The authors suggest the identified QTLs, especially qSTGL8.0, can increase out-crossing rate and seed production and decrease seed cost.
- Taxon
- Rice, O. sativa L.Oryza sativat0
- Gene
- qSTGL8.0e0
- Outcome
- increasing out-crossing ratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice, O. sativa L.”
e0“The newly identified QTLs especially qSTGL8.0, will be quite useful for increasing out-crossing rate and this will contribute to increase seed production and decrease seed cost”