Paper · 2021 · BIOTROPIC The Journal of Tropical Biology

Pengaruh Komposisi 2,4-D dan BAP Terhadap Pembentukan Kalus Eksplan Pucuk Nilam (Pogostemon Cablin Benth.) secara In Vitro dengan Pemotongan Horizontal dan Vertikal

Khairan Khairan; Betty Mauliya Bustam; Yunita; et al.

8
claims
16
verbatim quotes
2
citations
2
research areas
✓
Open access · cc-by-nc-sa

Abstract

This study aims to determine the effect of 2,4-Diclorophenoxy Acetic Acid (2,4-D) and Benzyl Amino Purin (BAP) on the formation of callus of patchouli (Pogostemon cablin Benth.) shoot explants by horizontal and vertical cutting methods. The parameters that observed in this study were the percentage growth of callus, time appearance of callus, weight of callus and the morphology of callus. The results showed that horizontal cutting method was able to induce callus growth with the percentages growth of callus were 18,75%, with the time appearance of callus was at 16 days at P1; P10; P12; P13 dan P14. The highest weight of callus obtained was 0.19 grams at P8. The results also showed that the callus yielded had a yellow and cream color, with a compact and crumb textures. Meanwhile, the vertical cutting method was able to induce callus formation with the percentage growth of callus were 12,5%. The fastest time of callus appearance was obtained in P6 and P8, which was 12 day after planting with the highest weight of callus obtained was 0.05 grams at P12. The results also showed that vertical cutting method had brown and dark-brown of callus with a compact and crumb textures.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Horizontal cutting induced patchouli callus growth in 18,75% of explants.

Taxon
patchouliPogostemon cabline1
Treatment
horizontal cutting methode0
Outcome
percentages growth of calluse0
Direction
inducee0
Value
18,75%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“horizontal cutting method was able to induce callus growth with the percentages growth of callus were 18,75%”

✓ located verbatim in the source · abstract, characters 416–524 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Horizontal cutting yielded callus at 16 days for P1, P10, P12, P13 and P14.

Taxon
patchouliPogostemon cabline1
Material
P1; P10; P12; P13 dan P14e0
Outcome
time appearance of calluse0
Value
16 dayse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“time appearance of callus was at 16 days at P1; P10; P12; P13 dan P14”

✓ located verbatim in the source · abstract, characters 535–604 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Horizontal cutting yielded the highest callus weight of 0.19 grams at P8.

Taxon
patchouliPogostemon cabline1
Material
P8e0
Outcome
weight of calluse0
Direction
higheste0
Value
0.19 gramse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest weight of callus obtained was 0.19 grams at P8”

✓ located verbatim in the source · abstract, characters 606–664 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Horizontal cutting yielded yellow and cream callus with compact and crumb textures.

Taxon
patchouliPogostemon cabline1
Outcome
yellow and cream color, with a compact and crumb texturese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the callus yielded had a yellow and cream color, with a compact and crumb textures”

✓ located verbatim in the source · abstract, characters 695–777 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Vertical cutting induced patchouli callus formation in 12,5% of explants.

Taxon
patchouliPogostemon cabline1
Treatment
vertical cutting methode0
Outcome
percentage growth of calluse0
Direction
inducee0
Value
12,5%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“vertical cutting method was able to induce callus formation with the percentage growth of callus were 12,5%”

✓ located verbatim in the source · abstract, characters 794–901 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Under vertical cutting, P6 and P8 had fastest callus appearance at 12 day after planting.

Taxon
patchouliPogostemon cabline1
Material
P6 and P8e0
Outcome
time of callus appearancee0
Direction
fasteste0
Value
12 daye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The fastest time of callus appearance was obtained in P6 and P8, which was 12 day after planting”

✓ located verbatim in the source · abstract, characters 903–999 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Under vertical cutting, the highest callus weight was 0.05 grams at P12.

Taxon
patchouliPogostemon cabline1
Material
P12e0
Outcome
weight of calluse0
Direction
higheste0
Value
0.05 gramse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“highest weight of callus obtained was 0.05 grams at P12”

✓ located verbatim in the source · abstract, characters 1009–1064 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vertical cutting yielded brown and dark-brown callus with compact and crumb textures.

Taxon
patchouliPogostemon cabline1
Outcome
brown and dark-brown of callus with a compact and crumb texturese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“vertical cutting method had brown and dark-brown of callus with a compact and crumb textures”

✓ located verbatim in the source · abstract, characters 1095–1187 · show in text ↑
e1

“patchouli (Pogostemon cablin Benth.) shoot explants”

✓ located verbatim in the source · abstract, characters 141–192 · show in text ↑

pruning · growth · Pogostemon cablin (patchouli)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.