Abstract
Tan spot of wheat, caused by Pyrenophora tritici-repentis (Ptr), results in a yield loss through chlorosis and necrosis of healthy leaf tissue. The major objective of this study was to compare gene expression in resistant and susceptible wheat cultivars after infection with Ptr ToxA-producing race 2 and direct infiltration with Ptr ToxA proteins. Greenhouse experiments included exposure of the wheat cultivars to pathogen inoculum or direct infiltration of leaf tissue with Ptr-ToxA protein isolate. Samples from the experiments were subjected to RNA sequencing. Results showed that ToxA RNA sequences were first detected in samples collected eight hours after treatments indicating that upon Ptr contact with wheat tissue, Ptr started expressing ToxA. The resistant wheat cultivar, in response to Ptr inoculum, expressed genes associated with plant resistance responses that were not expressed in the susceptible cultivar; genes of interest included five chitinases, eight transporters, five pathogen-detecting receptors, and multiple classes of signaling factors. Resistant and susceptible wheat cultivars therefore differed in their response in the expression of genes that encode chitinases, transporters, wall-associated kinases, permeases, and wound-induced proteins, among others. Plants exposed to Ptr inoculum expressed transcription factors, kinases, receptors, and peroxidases, which are not expressed as highly in the control samples or samples infiltrated with ToxA. Several of the differentially expressed genes between cultivars were found in the Ptr resistance QTLs on chromosomes 1A, 2D, 3B, and 5A. Future studies should elucidate the specific roles these genes play in the wheat response to Ptr.
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Claims 6
Claim · model-written summary
ToxA RNA was first detected eight hours after treatment.
- Taxon
- wheat
Triticum aestivumt0 - Interactor
- Pyrenophora tritici-repentis
Pyrenophora tritici-repentist0pathogen - Gene
- ToxAe0
- Outcome
- ToxA RNA sequencese0
- Value
- eight hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ToxA RNA sequences were first detected in samples collected eight hours after treatments”
t0“Pyrenophora tritici-repentis Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars”
Claim · model-written summary
Resistant wheat expressed resistance-response genes after inoculation that susceptible wheat did not express.
- Taxon
- wheatTriticum aestivume0
- Material
- resistant wheat cultivare0
- Treatment
- Ptr inoculume0
- Interactor
- Ptre0pathogen
- Outcome
- genes associated with plant resistance responsese0
- Direction
- not expressede0
- Comparator
- susceptible cultivare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resistant wheat cultivar, in response to Ptr inoculum, expressed genes associated with plant resistance responses that were not expressed in the susceptible cultivar”
Claim · model-written summary
Differential resistance-response genes included chitinases, transporters, receptors and signaling factors.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Pyrenophora tritici-repentisPyrenophora tritici-repentist0pathogen
- Outcome
- five chitinases, eight transporters, five pathogen-detecting receptors, and multiple classes of signaling factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes of interest included five chitinases, eight transporters, five pathogen-detecting receptors, and multiple classes of signaling factors”
t0“Pyrenophora tritici-repentis Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars”
Claim · model-written summary
Resistant and susceptible cultivars differed in expression of chitinases, transporters, wall-associated kinases, permeases and wound-induced proteins.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pyrenophora tritici-repentisPyrenophora tritici-repentist0pathogen
- Outcome
- expression of genes that encode chitinases, transporters, wall-associated kinases, permeases, and wound-induced proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Resistant and susceptible wheat cultivars therefore differed in their response in the expression of genes that encode chitinases, transporters, wall-associated kinases, permeases, and wound-induced proteins”
t0“Pyrenophora tritici-repentis Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars”
Claim · model-written summary
Ptr inoculum induced higher expression of transcription factors, kinases, receptors and peroxidases than controls or ToxA infiltration.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Ptr inoculume0
- Interactor
- Ptre0pathogen
- Outcome
- transcription factors, kinases, receptors, and peroxidasese0
- Direction
- not expressed as highlye0
- Comparator
- control samples or samples infiltrated with ToxAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants exposed to Ptr inoculum expressed transcription factors, kinases, receptors, and peroxidases, which are not expressed as highly in the control samples or samples infiltrated with ToxA”
t0“Pyrenophora tritici-repentis Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars”
Claim · model-written summary
Some genes differentially expressed between cultivars lay in Ptr-resistance QTLs on chromosomes 1A, 2D, 3B and 5A.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Ptre0pathogen
- Gene
- Ptr resistance QTLse0
- Outcome
- differentially expressed genes between cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several of the differentially expressed genes between cultivars were found in the Ptr resistance QTLs on chromosomes 1A, 2D, 3B, and 5A”
t0“Pyrenophora tritici-repentis Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
37 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- no effect
- no effect
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See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.