Abstract
Abstract Salinity stress has a significant impact on the gain of plant biomass. Our study provides the first root transcriptome of Cenostigma pyramidale , a tolerant woody legume from a tropical dry forest, under three different salt stress times (30 min, 2 h, and 11 days). The transcriptome was assembled using the RNA sequencing (RNA‐Seq) de novo pipeline from GenPipes. We observed 932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively. For DEGs annotated with the Viridiplantae clade in the early stress periods, the response to salt stress was mainly achieved by stabilizing homeostasis of such ions like Na + and K + , signaling by Ca 2+ , transcription factor modulation, water transport, and oxidative stress. For salt stress at 11 days, we observed a higher modulation of transcription factors including the WRKY , MYB , bHLH , NAC , HSF , and AP2‐EREBP families, as well as DEGs involved in hormonal responses, water transport, sugar metabolism, proline, and reactive oxygen scavenging mechanisms. Five selected DEGs (K + transporter, aquaporin, glutathione S‐transferase, cyclic nucleotide‐gated channel, and superoxide dismutase) were validated by qPCR. Our results indicated that C. pyramidale had an early perception of salt stress modulating ionic channels and transporters, and as the stress progressed, the focus turned to the antioxidant system, aquaporins, and complex hormone responses. The results of this first root transcriptome provide clues on how this native species modulate gene expression to achieve salt stress tolerance.
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Claims 11
Claim · model-written summary
The study provides the first root transcriptome of Cenostigma pyramidale under three salt stress times.
- Taxon
- Cenostigma pyramidale
Cenostigma pyramidalee0 - Outcome
- first root transcriptomee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our study provides the first root transcriptome of Cenostigma pyramidale”
Claim · model-written summary
932 upregulated DEGs were observed in C. pyramidale roots under salt stress for 30 min.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salte0
- Outcome
- upregulated differentially expressed genese0
- Value
- 932e0
- Qualifier
- 30 mine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively”
t0“Cenostigma pyramidale”
Claim · model-written summary
804 upregulated DEGs were observed in C. pyramidale roots under salt stress for 2 h.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salte0
- Outcome
- upregulated differentially expressed genese0
- Value
- 804e0
- Qualifier
- 2 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively”
t0“Cenostigma pyramidale”
Claim · model-written summary
3157 upregulated DEGs were observed in C. pyramidale roots under salt stress for 11 days.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salte0
- Outcome
- upregulated differentially expressed genese0
- Value
- 3157e0
- Qualifier
- 11 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively”
t0“Cenostigma pyramidale”
Claim · model-written summary
164 downregulated DEGs were observed in C. pyramidale roots under salt stress for 30 min.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salte0
- Outcome
- downregulated DEGse0
- Value
- 164e0
- Qualifier
- 30 mine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively”
t0“Cenostigma pyramidale”
Claim · model-written summary
273 downregulated DEGs were observed in C. pyramidale roots under salt stress for 2 h.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salte0
- Outcome
- downregulated DEGse0
- Value
- 273e0
- Qualifier
- 2 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively”
t0“Cenostigma pyramidale”
Claim · model-written summary
1332 downregulated DEGs were observed in C. pyramidale roots under salt stress for 11 days.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salte0
- Outcome
- downregulated DEGse0
- Value
- 1332e0
- Qualifier
- 11 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively”
t0“Cenostigma pyramidale”
Claim · model-written summary
In early salt stress periods, the response of C. pyramidale was mainly achieved by stabilizing ion homeostasis (Na+, K+), Ca2+ signaling, transcription factor modulation, water transport and oxidative stress.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salt stresse0
- Outcome
- response to salt stresse0
- Qualifier
- stabilizing homeostasis of such ions like Na + and K +e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the response to salt stress was mainly achieved by stabilizing homeostasis of such ions like Na + and K + , signaling by Ca 2+ , transcription factor modulation, water transport, and oxidative stress”
t0“Cenostigma pyramidale”
Claim · model-written summary
At 11 days of salt stress, C. pyramidale showed higher modulation of transcription factors including WRKY, MYB, bHLH, NAC and HSF families.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Treatment
- salt stress at 11 dayse0
- Outcome
- higher modulation of transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For salt stress at 11 days, we observed a higher modulation of transcription factors including the WRKY , MYB , bHLH , NAC , HSF”
t0“Cenostigma pyramidale”
Claim · model-written summary
At 11 days of salt stress, DEGs involved in hormonal responses, water transport, sugar metabolism, proline and reactive oxygen scavenging were also modulated.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Outcome
- DEGs involved in hormonal responses, water transport, sugar metabolism, proline, and reactive oxygen scavenging mechanismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as well as DEGs involved in hormonal responses, water transport, sugar metabolism, proline, and reactive oxygen scavenging mechanisms”
t0“Cenostigma pyramidale”
Claim · model-written summary
Five selected DEGs were validated by qPCR.
- Taxon
- Cenostigma pyramidaleCenostigma pyramidalet0
- Outcome
- validated by qPCRe1
- Qualifier
- Five selected DEGse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five selected DEGs (K + transporter, aquaporin”
e1“were validated by qPCR”
t0“Cenostigma pyramidale”