Abstract
Summary Nucleotide‐binding site (NBS)–leucine‐rich repeat (LRR) domain receptor (NLR) proteins play important roles in plant innate immunity by recognizing pathogen effectors. The Toll/interleukin‐1 receptor (TIR)‐NBS (TN) proteins belong to a subtype of the atypical NLRs, but their function in plant immunity is poorly understood. The well‐characterized Arabidopsis thaliana typical coiled‐coil (CC)‐NBS‐LRR (CNL) protein Resistance to Pseudomonas syringae 5 (RPS5) is activated after recognizing the Pseudomonas syringae type III effector AvrPphB. To explore whether the truncated TN proteins function in CNL‐mediated immune signaling, we examined the interactions between the Arabidopsis TN proteins and RPS5, and found that TN13 and TN21 interacted with RPS5. However, only TN13, but not TN21, was involved in the resistance to P . syringae pv. tomato ( Pto ) strain DC3000 carrying avrPphB , encoding the cognate effector recognized by RPS5. Moreover, the regulation of Pto DC3000 avrPphB resistance by TN13 appeared to be specific, as loss of function of TN13 did not compromise resistance to Pto DC3000 hrcC − or Pto DC3000 avrRpt2 . In addition, we demonstrated that the CC and NBS domains of RPS5 play essential roles in the interaction between TN13 and RPS5. Taken together, our results uncover a direct functional link between TN13 and RPS5, suggesting that TN13 acts as a partner in modulating RPS5‐activated immune signaling, which constitutes a previously unknown mechanism for TN‐mediated regulation of plant immunity.
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Claims 8
Claim · model-written summary
TN13 interacted with RPS5.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- TN13e0
- Outcome
- interacted with RPS5e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TN13 and TN21 interacted with RPS5.”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
Claim · model-written summary
TN21 interacted with RPS5.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- TN21e0
- Outcome
- interacted with RPS5e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TN13 and TN21 interacted with RPS5.”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
Claim · model-written summary
TN13 contributed to resistance against Pto DC3000 carrying avrPphB.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- P . syringae pv. tomato ( Pto ) strain DC3000
Pseudomonas syringae pv. tomatoe0pathogen - Gene
- TN13e0
- Outcome
- resistance to P . syringae pv. tomato ( Pto ) strain DC3000 carrying avrPphBe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only TN13, but not TN21, was involved in the resistance to P . syringae pv. tomato ( Pto ) strain DC3000 carrying avrPphB”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
Claim · model-written summary
TN21 was not involved in resistance against Pto DC3000 carrying avrPphB.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- P . syringae pv. tomato ( Pto ) strain DC3000Pseudomonas syringae pv. tomatoe0pathogen
- Gene
- TN21e0
- Outcome
- resistance to P . syringae pv. tomato ( Pto ) strain DC3000 carrying avrPphBe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only TN13, but not TN21, was involved in the resistance to P . syringae pv. tomato ( Pto ) strain DC3000 carrying avrPphB”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
Claim · model-written summary
TN13 loss of function did not compromise resistance to Pto DC3000 hrcC −.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- loss of function of TN13e0
- Interactor
- Pto DC3000 hrcC −e0pathogen
- Outcome
- resistance to Pto DC3000 hrcC −e0
- Direction
- did not compromisee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of function of TN13 did not compromise resistance to Pto DC3000 hrcC −”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
Claim · model-written summary
TN13 loss of function did not compromise resistance to Pto DC3000 avrRpt2.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- loss of function of TN13e0
- Interactor
- Pto DC3000 avrRpt2e0pathogen
- Outcome
- Pto DC3000 avrRpt2e0
- Direction
- did not compromisee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of function of TN13 did not compromise resistance to Pto DC3000 hrcC − or Pto DC3000 avrRpt2”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
Claim · model-written summary
The CC domain of RPS5 was essential for TN13-RPS5 interaction.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RPS5e0
- Outcome
- interaction between TN13 and RPS5e0
- Qualifier
- CCe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CC and NBS domains of RPS5 play essential roles in the interaction between TN13 and RPS5”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
Claim · model-written summary
The NBS domain of RPS5 was essential for TN13-RPS5 interaction.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RPS5e0
- Outcome
- interaction between TN13 and RPS5e0
- Qualifier
- NBSe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CC and NBS domains of RPS5 play essential roles in the interaction between TN13 and RPS5”
t0“The TIR‐NBS protein TN13 associates with the CC‐NBS‐LRR resistance protein RPS5 and contributes to RPS5‐triggered immunity in Arabidopsis”
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loss of function · disease · ArabidopsisPositive and negative results
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