Abstract
Toll/interleukin receptor (TIR)-nucleotide binding site (NB)-type (TN) proteins are encoded by a family of 21 genes in the Arabidopsis genome. Previous studies have shown that a mutation in the TN gene CHS1 activates the activation of defense responses at low temperatures. However, the underlying molecular mechanism remains unknown. To genetically dissect chs1-mediated signaling, we isolated genetic suppressors of chs1-2 (soc). Several independent soc mutants carried mutations in the same TIR-NB-leucine-rich repeat (LRR) (TNL)-encoding gene SOC3, which is adjacent to CHS1 on chromosome 1. Expression of SOC3 was upregulated in the chs1-2 mutant. Mutations in six soc3 alleles and downregulation of SOC3 by an artificial microRNA construct fully rescued the chilling sensitivity and defense defects of chs1-2. Biochemical studies showed that CHS1 interacted with the NB and LRR domains of SOC3; however, mutated chs1 interacted with the TIR, NB and LRR domains of SOC3 in vitro and in vivo. This study reveals that the TN protein CHS1 interacts with the TNL protein SOC3 to modulate temperature-dependent autoimmunity.
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Claims 7
Claim · model-written summary
Independent chs1 suppressor mutants carried mutations in SOC3 adjacent to CHS1.
Not reportedTaxon
Verbatim
e0“Several independent soc mutants carried mutations in the same TIR-NB-leucine-rich repeat (LRR) (TNL)-encoding gene SOC3, which is adjacent to CHS1 on chromosome 1.”
t0“chs1”
Claim · model-written summary
SOC3 expression was upregulated in the chs1-2 mutant.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- SOC3e0
- Outcome
- Expression of SOC3e0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of SOC3 was upregulated in the chs1-2 mutant.”
t0“Arabidopsis genome”
Claim · model-written summary
Mutations in six soc3 alleles rescued chs1-2 chilling sensitivity and defense defects.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Mutations in six soc3 allelese0
- Gene
- soc3e0
- Outcome
- chilling sensitivity and defense defectse0
- Direction
- fully rescuede0
- Comparator
- chs1-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in six soc3 alleles and downregulation of SOC3 by an artificial microRNA construct fully rescued the chilling sensitivity and defense defects of chs1-2.”
t0“Arabidopsis genome”
Claim · model-written summary
Artificial microRNA downregulation of SOC3 rescued chs1-2 chilling sensitivity and defense defects.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- downregulation of SOC3 by an artificial microRNA constructe0
- Gene
- SOC3e0
- Outcome
- chilling sensitivity and defense defectse0
- Direction
- fully rescuede0
- Comparator
- chs1-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulation of SOC3 by an artificial microRNA construct fully rescued the chilling sensitivity and defense defects of chs1-2.”
t0“Arabidopsis genome”
Claim · model-written summary
CHS1 interacted with the NB and LRR domains of SOC3.
Not reportedTaxon
Verbatim
e0“CHS1 interacted with the NB and LRR domains of SOC3”
Claim · model-written summary
Mutated chs1 interacted with TIR, NB and LRR domains of SOC3 in vitro and in vivo.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- chs1e0
- Outcome
- interacted with the TIR, NB and LRR domains of SOC3e0
- Setting
- in vitro and in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutated chs1 interacted with the TIR, NB and LRR domains of SOC3 in vitro and in vivo”
t0“Arabidopsis genome”
Claim · model-written summary
The authors conclude that CHS1-SOC3 interaction modulates temperature-dependent autoimmunity.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CHS1e0
- Outcome
- temperature-dependent autoimmunitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the TN protein CHS1 interacts with the TNL protein SOC3 to modulate temperature-dependent autoimmunity”
t0“Arabidopsis genome”
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loss of function · disease · ArabidopsisPositive and negative results
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