Abstract
Verticillium dahliae is a hemibiotrophic pathogen responsible for great losses in dicot crop production. An ExoPG gene (VDAG_03463,) identified using subtractive hybridization/cDNA-AFLP, showed higher expression levels in highly aggressive than in weakly aggressive V. dahliae isolates. We used a vector-free split-marker recombination method with PEG-mediated protoplast to delete the ExoPG gene in V. dahliae. This is the first instance of using this method for V. dahliae transformation. Only two PCR steps and one transformation step were required, markedly reducing the necessary time for gene deletion. Six mutants were identified. ExoPG expressed more in the highly aggressive than in the weakly aggressive isolate in response to potato leaf and stem extracts. Its expression increased in both isolates during infection, with higher levels in the highly aggressive isolate at early infection stages. The disruption of ExoPG did not influence virulence, nor did it affect total exopolygalacturonase activity in V. dahliae. Full genome analysis showed 8 more genes related to polygalacturonase/pectinase activity in V. dahliae. Transcripts of PGA increased in the △exopg mutant in response to potato leaf extracts, compared to the wild type. The expression pattern of those eight genes showed similar trends in the △exopg mutant and in the weakly aggressive isolate in response to potato extracts, but without the increase of PGA in the weakly aggressive isolate to leaf extracts. This indicated that the △exopg mutant of V. dahliae compensated by the suppression of ExoPG by activating other related gene.
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Claims 9
Claim · model-written summary
ExoPG expression was higher in highly aggressive isolates than weakly aggressive isolates in response to potato leaf and stem extracts.
- Taxon
- Potato
Solanum tuberosumt0 - Organ / stage
- leaf and steme0
- Treatment
- potato leaf and stem extractse0
- Interactor
- Verticillium dahliae
Verticillium dahliaet0pathogen - Gene
- ExoPGe0
- Outcome
- ExoPG expressede0
- Direction
- moree0
- Comparator
- weakly aggressive isolatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ExoPG expressed more in the highly aggressive than in the weakly aggressive isolate in response to potato leaf and stem extracts.”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
ExoPG expression increased during infection in both isolates and was higher in the aggressive isolate early in infection.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Verticillium dahliaeVerticillium dahliaet0pathogen
- Gene
- ExoPGe1
- Outcome
- expressione0
- Direction
- increasede0
- Qualifier
- early infection stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its expression increased in both isolates during infection, with higher levels in the highly aggressive isolate at early infection stages.”
e1“ExoPG expressed more”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
ExoPG disruption did not influence virulence.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- disruption of ExoPGe0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- ExoPGe0
- Outcome
- virulencee0
- Direction
- did not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disruption of ExoPG did not influence virulence, nor did it affect total exopolygalacturonase activity in V. dahliae.”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
ExoPG disruption did not affect total fungal exopolygalacturonase activity.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- disruption of ExoPGe0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- ExoPGe0
- Outcome
- total exopolygalacturonase activity in V. dahliaee0
- Direction
- did it affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disruption of ExoPG did not influence virulence, nor did it affect total exopolygalacturonase activity in V. dahliae.”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
Genome analysis identified eight more fungal genes related to polygalacturonase or pectinase activity.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Outcome
- genes related to polygalacturonase/pectinase activity in V. dahliaee0
- Value
- 8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Full genome analysis showed 8 more genes related to polygalacturonase/pectinase activity in V. dahliae.”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
PGA transcripts increased in the ExoPG deletion mutant after potato leaf extract exposure relative to wild type.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- △exopg mutante0
- Treatment
- potato leaf extractse0
- Interactor
- Verticillium dahliaeVerticillium dahliaet0pathogen
- Gene
- PGAe0
- Outcome
- Transcripts of PGAe0
- Direction
- increasede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcripts of PGA increased in the △exopg mutant in response to potato leaf extracts, compared to the wild type.”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
Eight related genes showed similar expression trends in the ExoPG mutant and weakly aggressive isolate after potato extracts.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- △exopg mutante0
- Treatment
- potato extractse0
- Interactor
- Verticillium dahliaeVerticillium dahliaet0pathogen
- Outcome
- expression pattern of those eight genese0
- Direction
- similar trendse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression pattern of those eight genes showed similar trends in the △exopg mutant and in the weakly aggressive isolate in response to potato extracts”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
The weakly aggressive isolate lacked the PGA increase to potato leaf extract seen in the mutant.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- leaf extractse0
- Interactor
- Verticillium dahliaeVerticillium dahliaet0pathogen
- Gene
- PGAe0
- Outcome
- increase of PGA in the weakly aggressive isolatee0
- Direction
- without the increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“without the increase of PGA in the weakly aggressive isolate to leaf extracts.”
t0“Potato-Verticillium dahliae”
Claim · model-written summary
Authors infer that the deletion mutant compensates for ExoPG suppression by activating related genes.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- △exopg mutante0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- ExoPGe0
- Outcome
- compensated by the suppression of ExoPGe0
- Qualifier
- activating other related genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This indicated that the △exopg mutant of V. dahliae compensated by the suppression of ExoPG by activating other related gene.”
t0“Potato-Verticillium dahliae”
The same questions in other papers
loss of function · Verticillium dahliae · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.