Paper · 2021 · BIOEDUSCIENCE

The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions

Turhadi; Miftahuddin; Hamim; et al.

7
claims
12
verbatim quotes
2
citations
3
research areas
✓
Open access · cc-by

Abstract

Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice under Fe toxicity stress condition. Methods: This experiment was carried out by comparing the effectiveness of three kinds of nutrient culture media, namely Yoshida’s Half-Strength solution (HSY), Yoshida’s Half-Strength + 0.2% agar solution (HSYA), and Yoshida’s Full-Strength + 0.2% agar solution (FSYA) using two rice genotypes, Inpara 5 (sensitive to Fe toxicity) and Mahsuri (tolerant to Fe toxicity). Leaf bronzing level, plant dry weight, and pH of nutrient culture media were observed in this experiment. Results: The results showed that the stress response as represented by the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media. The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri. In addition, the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA). Conclusion: The HSYA and FSYA media exhibited a similar pattern of pH declining but causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Inpara 5 had a higher leaf bronzing score than Mahsuri in all three media.

Material
Inpara 5e0
Outcome
bronzing scoree0
Direction
highere0
Qualifier
three nutrient culture mediae0
Comparator
Mahsurie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media”

✓ located verbatim in the source · abstract, characters 884–1001 · show in text ↑
t0

“Rice”

✓ located verbatim in the source · title, characters 93–97 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The root dry-weight decrease was greater in Inpara 5 than Mahsuri.

Material
Inpara 5e0
Organ / stage
roote0
Outcome
root and shoot dry weighte0
Direction
decreasee0
Comparator
Mahsurie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri”

✓ located verbatim in the source · abstract, characters 1003–1088 · show in text ↑
t0

“Rice”

✓ located verbatim in the source · title, characters 93–97 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The shoot dry-weight decrease was greater in Inpara 5 than Mahsuri.

Material
Inpara 5e0
Organ / stage
shoote0
Outcome
root and shoot dry weighte0
Direction
decreasee0
Comparator
Mahsurie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri”

✓ located verbatim in the source · abstract, characters 1003–1088 · show in text ↑
t0

“Rice”

✓ located verbatim in the source · title, characters 93–97 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Medium pH fell faster without agar than with agar.

Treatment
without an agar addition (HSY)e0
Outcome
pH of nutrient culture solution mediae0
Direction
decreasee0
Comparator
media with the agar addition (HSYA and FSYA)e0

Not reportedTaxon

Verbatim

e0

“the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA)”

✓ located verbatim in the source · abstract, characters 1103–1261 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

HSYA and FSYA showed similar pH decline patterns.

Outcome
pH declininge0
Direction
similare0

Not reportedTaxonMaterial

Verbatim

e0

“The HSYA and FSYA media exhibited a similar pattern of pH declining”

✓ located verbatim in the source · abstract, characters 1275–1342 · show in text ↑
Method / resourceobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

HSYA and FSYA caused significant differences in growth responses between Inpara 5 and Mashuri.

Material
Inpara 5 and Mashurie0
Treatment
HSYA mediume0
Outcome
growth responsese0
Comparator
FSYA mediume0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar”

✓ located verbatim in the source · abstract, characters 1347–1561 · show in text ↑
t0

“Rice”

✓ located verbatim in the source · title, characters 93–97 · show in text ↑
Method / resourceauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

HSYA was judged more efficient than FSYA because it required less agar.

Treatment
HSYA mediume0
Outcome
more efficiente0
Direction
more efficiente0
Qualifier
smaller amount of agare0
Comparator
FSYA mediume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar”

✓ located verbatim in the source · abstract, characters 1439–1561 · show in text ↑
t0

“Rice”

✓ located verbatim in the source · title, characters 93–97 · show in text ↑