Abstract
Solanum lycopersicum phenylalanine ammonia-lyase 5 (SlPAL5) gene regulates the metabolism of phenolic compounds. The study of transcription factors that regulate the expression of SlPAL5 gene is of great significance to elucidate the regulatory mechanism underlying the biosynthesis of phenolic compounds in tomato fruit induced by UV-C irradiation. Here, yeast one-hybrid library of tomato fruit was constructed, and the yeast one-hybrid technology was used to screen the transcription factors that regulate the expression of SlPAL5, the key gene related to the synthesis of phenolic compounds in tomato fruit. As a result, a transcription factor, SlERF7, was obtained and sequenced, followed by the blast homology analysis. Further experiments confirmed that SlERF7 interacted with the promoter of SlPAL5 gene. In addition, UV-C irradiation significantly increased the expression level of SlERF7. These results indicate that SlERF7, which is regulated by UV-C irradiation, might be involved in regulating the transcription of SlPAL5, which provided foundations for further studying the regulation mechanism of the biosynthesis of phenolic compounds in tomato fruit induced by UV-C irradiation.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
SlERF7 was identified and sequenced by the yeast one-hybrid screen.
- Taxon
- tomato fruit
Solanum lycopersicumt0 - Gene
- SlERF7e0
- Outcome
- obtained and sequencede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a transcription factor, SlERF7, was obtained and sequenced”
t0“tomato fruit”
Claim · model-written summary
SlERF7 interacted with the SlPAL5 promoter.
- Taxon
- tomato fruitSolanum lycopersicumt0
- Gene
- SlERF7e0
- Outcome
- interacted with the promoter of SlPAL5 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlERF7 interacted with the promoter of SlPAL5 gene”
t0“tomato fruit”
Claim · model-written summary
UV-C irradiation significantly increased SlERF7 expression.
- Taxon
- tomato fruitSolanum lycopersicumt0
- Treatment
- UV-C irradiatione0
- Gene
- SlERF7e0
- Outcome
- expression level of SlERF7e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV-C irradiation significantly increased the expression level of SlERF7”
t0“tomato fruit”
Claim · model-written summary
The authors infer that UV-C-regulated SlERF7 might regulate SlPAL5 transcription.
- Taxon
- tomato fruitSolanum lycopersicumt0
- Gene
- SlERF7e0
- Outcome
- transcription of SlPAL5e0
- Qualifier
- might be involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlERF7, which is regulated by UV-C irradiation, might be involved in regulating the transcription of SlPAL5”
t0“tomato fruit”
The same questions in other papers
uv · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.