Abstract
The connection between olive genetic responses to environmental and agro-climatic conditions and the composition, structure and functioning of host-associated, belowground microbiota has never been studied under the holobiont conceptual framework. Two groups of cultivars growing under the same environmental, pedological and agronomic conditions, and showing highest (AH) and lowest (AL) Actinophytocola relative abundances, were earlier identified. We aimed now to: i) compare the root transcriptome profiles of these two groups harboring significantly different relative abundances in the above-mentioned bacterial genus; ii) examine their rhizosphere and root-endosphere microbiota co-occurrence networks; and iii) connect the root host transcriptome pattern to the composition of the root microbial communities by correlation and co-occurrence network analyses. Significant differences in olive gene expression were found between the two groups. Co-occurrence networks of the root endosphere microbiota were clearly different as well. Pearson’s correlation analysis enabled a first portray of the interaction occurring between the root host transcriptome and the endophytic community. To further identify keystone operational taxonomic units (OTUs) and genes, subsequent co-occurrence network analysis showed significant interactions between 32 differentially expressed genes (DEGs) and 19 OTUs. Overall, negative correlation was detected between all upregulated genes in the AH group and all OTUs except of Actinophytocola. While two groups of olive cultivars grown under the same conditions showed significantly different microbial profiles, the most remarkable finding was to unveil a strong correlation between these profiles and the differential gene expression pattern of each group. In conclusion, this study shows a holistic view of the plant-microbiome communication.
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Claims 7
Claim · model-written summary
Olive gene expression differed significantly between the two cultivar groups.
- Taxon
- olive
Olea europaeae0 - Material
- cultivarse1
- Interactor
- ActinophytocolaActinophytocolae1other
- Outcome
- gene expressione0
- Qualifier
- same environmental, pedological and agronomic conditionse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant differences in olive gene expression were found between the two groups.”
e1“Two groups of cultivars growing under the same environmental, pedological and agronomic conditions, and showing highest (AH) and lowest (AL) Actinophytocola relative abundances”
Claim · model-written summary
Root endosphere microbiota co-occurrence networks differed between groups.
- Taxon
- oliveOlea europaeat0
- Interactor
- root endosphere microbiotae0other
- Outcome
- Co-occurrence networks of the root endosphere microbiotae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“olive roots”
e0“Co-occurrence networks of the root endosphere microbiota were clearly different as well.”
Claim · model-written summary
Pearson correlation portrayed an interaction between root host transcriptome and endophytic community.
- Taxon
- oliveOlea europaeat0
- Interactor
- endophytic communitye0other
- Outcome
- root host transcriptomee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“olive roots”
e0“Pearson’s correlation analysis enabled a first portray of the interaction occurring between the root host transcriptome and the endophytic community.”
Claim · model-written summary
Network analysis showed significant interactions between 32 differentially expressed genes and 19 OTUs.
- Taxon
- oliveOlea europaeat0
- Gene
- differentially expressed genese0
- Outcome
- interactionse0
- Value
- 32e0
- Qualifier
- 19 OTUse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“olive roots”
e0“subsequent co-occurrence network analysis showed significant interactions between 32 differentially expressed genes (DEGs) and 19 OTUs.”
Claim · model-written summary
All upregulated genes in AH were negatively correlated with all OTUs except Actinophytocola.
- Taxon
- oliveOlea europaeat0
- Material
- AH groupe0
- Outcome
- all upregulated genese0
- Direction
- negativee0
- Qualifier
- except of Actinophytocolae0
.jpg?width=60){kind=small}
OtusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“olive roots”
e0“Overall, negative correlation was detected between all upregulated genes in the AH group and all OTUs except of Actinophytocola.”
Claim · model-written summary
Olive cultivar groups under the same conditions had significantly different microbial profiles.
- Taxon
- oliveOlea europaeae0
- Material
- cultivarse0
- Outcome
- microbial profilese0
- Qualifier
- same conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two groups of olive cultivars grown under the same conditions showed significantly different microbial profiles”
Claim · model-written summary
Microbial profiles were strongly correlated with each group's differential gene expression pattern.
- Taxon
- oliveOlea europaeat0
- Outcome
- differential gene expression patterne0
- Qualifier
- strong correlatione0
- Comparator
- these profilese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“olive roots”
e0“the most remarkable finding was to unveil a strong correlation between these profiles and the differential gene expression pattern of each group.”