Abstract
To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/L 2.4-D (2,4-dichlorophenoxy acetic acid). The highest regeneration efficiency was achieved on MS medium (17 plantlets) with 8 mg/L 2,4-D. Endosperm-supported mature embryos of orchardgrass can be successfully used as explants. Therefore, this protocol provides a basis for future studies on genetic improvement and in vitro mutation studies.
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Claims 4
Claim · model-written summary
Chu medium with 12 mg/L dicamba gave the optimum callus induction response.
- Taxon
- orchardgrass
.jpg?width=60)
Dactylis glomeratat0 - Treatment
- Chu medium with 12 mg/L dicambae0
- Outcome
- callus inductione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid)”
t0“orchardgrass (Dactylis glomerata L.)”
Claim · model-written summary
Murashige and Skoog medium with 8 mg/L 2.4-D yielded the highest responding embryogenic callus, 15%.
- Taxon
- orchardgrassDactylis glomeratat0
- Treatment
- Murashige and Skoog medium and 8 mg/L 2.4-De0
- Outcome
- responding embryogenic calluse0
- Direction
- higheste0
- Value
- 15%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/L 2.4-D”
t0“orchardgrass (Dactylis glomerata L.)”
Claim · model-written summary
MS medium with 8 mg/L 2,4-D produced the highest regeneration efficiency, 17 plantlets.
- Taxon
- orchardgrassDactylis glomeratat0
- Treatment
- MS mediume0
- Outcome
- regeneration efficiencye0
- Direction
- higheste0
- Value
- 17 plantletse0
- Qualifier
- 8 mg/L 2,4-De0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest regeneration efficiency was achieved on MS medium (17 plantlets) with 8 mg/L 2,4-D”
t0“orchardgrass (Dactylis glomerata L.)”
Claim · model-written summary
Endosperm-supported mature embryos can be used successfully as orchardgrass explants.
- Taxon
- orchardgrassDactylis glomeratae0
- Organ / stage
- Endosperm-supported mature embryose0
- Outcome
- successfully used as explantse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endosperm-supported mature embryos of orchardgrass can be successfully used as explants.”
The same questions in other papers
dicamba · growth · Dactylis glomerata (cocksfoot)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Dactylis glomerata (cocksfoot)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.