Abstract
In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92％ when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61％. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69％. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.
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Claims 7
Claim · model-written summary
Embryogenic calli induced on MS medium with 3mg/L 2,4-D and 0.1mg/L BA had significantly better regeneration ability.
- Taxon
- orchardgrass
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Dactylis glomeratat0 - Treatment
- MS medium containing 3mg/L 2,4-D and 0.1mg/L BAe0
- Outcome
- regeneration abilitye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability.”
t0“Orchardgrass”
Claim · model-written summary
Regeneration reached 92％ on N6 medium with 1mg/L 2,4-D and 3mg/L BA.
- Taxon
- orchardgrassDactylis glomeratat0
- Treatment
- N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BAe0
- Outcome
- Plant regeneration ratee0
- Value
- 92％e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plant regeneration rate was 92％ when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA.”
t0“Orchardgrass”
Claim · model-written summary
MS medium was optimal for embryogenic callus induction.
- Taxon
- orchardgrassDactylis glomeratat0
- Treatment
- MSe0
- Outcome
- embryogenic callus inductione0
- Direction
- optimale0
- Comparator
- N6 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively.”
t0“Orchardgrass”
Claim · model-written summary
N6 medium was optimal for plant regeneration.
- Taxon
- orchardgrassDactylis glomeratat0
- Treatment
- N6 mediume0
- Outcome
- plant regeneratione0
- Direction
- optimale0
- Comparator
- MSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively.”
t0“Orchardgrass”
Claim · model-written summary
Callus induction frequency did not differ among four orchardgrass cultivars.
- Taxon
- orchardgrassDactylis glomeratae0
- Material
- four cultivarse0
- Outcome
- callus induction frequencye0
- Direction
- Ho differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ho difference in callus induction frequency was observed among four cultivars of orchardgrass”
Claim · model-written summary
Roughrider had higher regenerability at 61％.
- Taxon
- orchardgrassDactylis glomeratat0
- Material
- Roughridere0
- Outcome
- regenerabilitye0
- Direction
- highere0
- Value
- 61％e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“"Roughrider" cultivar showed higher regenerability with the frequency of 61％.”
t0“Orchardgrass”
Claim · model-written summary
Maltose increased regeneration frequency to 69％.
- Taxon
- orchardgrassDactylis glomeratat0
- Treatment
- Addition of maltosee0
- Outcome
- regeneration frequencye0
- Direction
- increasede0
- Value
- 69％e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69％.”
t0“Orchardgrass”
The same questions in other papers
N-benzyladenine · growth · Dactylis glomerata (cocksfoot)Positive and negative results
1 in the opposite direction
- no effect
2,4-D · growth · Dactylis glomerata (cocksfoot)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Dactylis glomerata (cocksfoot)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.