Abstract
Impatiens walleriana Hook.f. is a popular bedding plant with a variety of flower colours excepting yellow. Transfer of this characteristic could be achieved through somatic hybridization, thus this study was an attempt to establish the basic parameters of tissue and protoplast culture. The condition of the stock plants and surface sterilisation techniques are important for this species. Most tissues responded to trials for callus induction with rhizogenesis. Evaluation of media and plant growth regulator combinations revealed that axillary shoot proliferation was most efficient on MS medium with 3.0 mg/L BAP and 0.1 mg/L IBA. Protoplast isolation was most effective in 0.5% w/v cellulase and 0.25% w/v pectinase with 0.25 M mannitol and 0.25 M glucose yielding 2.70 $ times$ 10$ sp5$ protoplasts per gram of leaf tissue. The presence of mucous, raphids and excessive debris inhibited successful culture of leaf mesophyll protoplasts. Cotyledon protoplasts show promise for such investigations.
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Claims 5
Claim · model-written summary
Most tested tissues formed roots during callus induction trials.
- Taxon
- Impatiens walleriana
Impatiens wallerianat0 - Outcome
- rhizogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most tissues responded to trials for callus induction with rhizogenesis”
t0“Impatiens walleriana”
Claim · model-written summary
Axillary shoot proliferation was most efficient on MS medium containing 3.0 mg/L BAP and 0.1 mg/L IBA.
- Taxon
- Impatiens wallerianaImpatiens wallerianat0
- Treatment
- MS medium with 3.0 mg/L BAP and 0.1 mg/L IBAe0
- Outcome
- axillary shoot proliferatione0
- Direction
- most efficiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“axillary shoot proliferation was most efficient on MS medium with 3.0 mg/L BAP and 0.1 mg/L IBA”
t0“Impatiens walleriana”
Claim · model-written summary
The stated enzyme and osmoticum mixture gave the most effective leaf protoplast isolation, yielding 2.70 $ times$ 10$ sp5$ protoplasts per gram of leaf tissue.
- Taxon
- Impatiens wallerianaImpatiens wallerianat0
- Treatment
- 0.5% w/v cellulase and 0.25% w/v pectinase with 0.25 M mannitol and 0.25 M glucosee0
- Outcome
- Protoplast isolatione0
- Direction
- most effectivee0
- Value
- 2.70 $ times$ 10$ sp5$ protoplasts per gram of leaf tissuee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protoplast isolation was most effective in 0.5% w/v cellulase and 0.25% w/v pectinase with 0.25 M mannitol and 0.25 M glucose yielding 2.70 $ times$ 10$ sp5$ protoplasts per gram of leaf tissue”
t0“Impatiens walleriana”
Claim · model-written summary
Mucous, raphids and debris inhibited culture of leaf mesophyll protoplasts.
- Taxon
- Impatiens wallerianaImpatiens wallerianat0
- Outcome
- successful culture of leaf mesophyll protoplastse0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of mucous, raphids and excessive debris inhibited successful culture of leaf mesophyll protoplasts”
t0“Impatiens walleriana”
Claim · model-written summary
Cotyledon protoplasts were considered promising for culture investigations.
- Taxon
- Impatiens wallerianaImpatiens wallerianat0
- Outcome
- Cotyledon protoplasts show promisee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledon protoplasts show promise for such investigations”
t0“Impatiens walleriana”
The same questions in other papers
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- positive
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in vitro · growth · Impatiens wallerianaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.