Abstract
This study aimed to screen myxobacteria strains with strong activity against Phytophthora infestans and to study its secondary metabolites. Myxobacteria were isolatedfrom a soil sample using the rabbit feces as bait. A strain inhibitive to P. infestanswas identified by morphological observation and 16S rRNA sequence analysis. The fermentation parameters were optimized through a combination of univariate analysis and orthogonal optimization. The stability and antimicrobialactivities of the secondary metabolites were determined using the filter paper method, and the effects on the development of the potato late blight and its pathogen were determined using the in vitroleaf method. The myxobacteria B25-I-1 isolated from a soil sample showed strong activity against P. infestansand was identified as Myxococcus xanthus. The optimal fermentation conditions of M. xanthusB25-I-1 were as follows: inoculum size of 10%, shaking at 180 rmin-1, incubation temperature of 32°C, and incubation time of 8 days. The secondary metabolites were highly tolerant to high temperature, treatment with protease K, andultraviolet and natural lights. Besides P. infestans, the secondary metabolites also showed variant antagonistic effects againstEscherichia coli,Staphylococcus aureus,Bacillus subtilis,Saccharomyces cerevisiae,Fusarium oxysporum, Rhizoctonia solani, and Sclerotinia sclerotiorum. The results of the potato late blight control on potato leaves indicated that the secondary metabolites could significantly inhibit the infection of detached potato leaves caused by P. infestansand caused no damage to the leaves of different varieties of potatoes by themselves. This study provides basic data for the the development of a biopesticide against potato late blight.© 2021 Friends Science Publishers
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Claims 6
Claim · model-written summary
Myxobacteria strain B25-I-1 isolated from soil showed strong activity against Phytophthora infestans and was identified as Myxococcus xanthus.
- Taxon
- Myxococcus xanthus
Myxococcus xanthuse0 - Interactor
- P. infestans
Phytophthora infestanse0pathogen - Outcome
- strong activity against P. infestanse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The myxobacteria B25-I-1 isolated from a soil sample showed strong activity against P. infestansand was identified as Myxococcus xanthus”
Claim · model-written summary
The optimal fermentation conditions of M. xanthus B25-I-1 were inoculum size of 10%, shaking at 180 r/min, 32 C and 8 days of incubation.
Verbatim
e0“The optimal fermentation conditions of M. xanthusB25-I-1 were as follows: inoculum size of 10%, shaking at 180 rmin-1, incubation temperature of 32°C, and incubation time of 8 days”
Claim · model-written summary
The secondary metabolites were highly tolerant to high temperature, protease K treatment, and ultraviolet and natural light.
- Outcome
- highly tolerant to high temperature, treatment with protease K, andultraviolet and natural lightse0
Not reportedTaxon
Verbatim
e0“The secondary metabolites were highly tolerant to high temperature, treatment with protease K, andultraviolet and natural lights”
Claim · model-written summary
The secondary metabolites also showed variable antagonistic effects against E. coli, S. aureus, B. subtilis, S. cerevisiae, Fusarium oxysporum, Rhizoctonia solani and Sclerotinia sclerotiorum.
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Second interactor
- Rhizoctonia solani
Rhizoctonia solanie0pathogen - Outcome
- variant antagonistic effectse0
- Qualifier
- Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Saccharomyces cerevisiaee0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“the secondary metabolites also showed variant antagonistic effects againstEscherichia coli,Staphylococcus aureus,Bacillus subtilis,Saccharomyces cerevisiae,Fusarium oxysporum, Rhizoctonia solani, and Sclerotinia sclerotiorum”
Claim · model-written summary
The secondary metabolites significantly inhibited infection of detached potato leaves by P. infestans.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- the secondary metabolitese0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- infection of detached potato leavese0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the secondary metabolites could significantly inhibit the infection of detached potato leaves caused by P. infestans”
Claim · model-written summary
The secondary metabolites by themselves caused no damage to the leaves of different potato varieties.
- Taxon
- potatoesSolanum tuberosume0
- Outcome
- damage to the leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“caused no damage to the leaves of different varieties of potatoes by themselves”
The same questions in other papers
metabolite · Phytophthora infestans · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.